| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We examined the therapeutic effects of bacteriophage eye drop on methicillin-resistant Staphylococcus aureus keratitis in rabbit. The clinical corneal score was significantly lower in rabbits treated by bacteriophage eye drop than in those treated by anti-biotic eye drop or control. The bacterial load in cornea among the vehicle-treated rabbit, anti-biotic-treated rabbit, and bacteriophage-treated rabbit were not significantly changed.
We then examined the bacterial endophthalmitis in rabbit and mouse. The injection Pseudomonas aeruginosa or Enterococcus faecalis into vitreous cavity in mice stably induced severe endophthalmitis including massive infiltration of neutrophils and destruction of tissue. Injection of phage into vitreous cavity in mice with endophthalmitis significantly suppressed clinical score and viable bacteria in the eyeball.
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