Capability of vitreous fluid to enhance TGF-beta-induced Foxp3+ regulatory T cell conversion
| Project/Area Number |
26462696
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Kyorin University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 眼免疫 / 免疫学 |
|---|
| Outline of Final Research Achievements |
In the present study, we determined whether vitreous fluid can promote the generation of Foxp3+ CD4+ T regulatory (Treg) cells in vitro. Vitreous fluid was collected from fresh porcine eyes, sterilized by filtration. Vitreous was used for culture at a final concentration of 25%. CD4+ T cells purified from pooled splenocytes from naive C57Bl/6J were stimulated with plate-bound anti-CD3 and anti-CD28 antibodies for 96h in the presence or absence of vitreous fluid. CD4+ T cells were analyzed for Foxp3 expression using flow cytometry. In some experiments, CD4+ T cells were cultured in the presence of vitreous fluid with or without TGF-beta1 or 2. Vitreous fluid alone did not increase the frequency of Foxp3+ Tregs. Although TGF-beta 1 or 2 induced Foxp3+ Tregs in vitro, the frequency of TGF-beta1/2 induced Foxp3+ Tregs increased significantly in the presence of vitreous fluid. These findings suggest that vitreous fluid may enhance TGF-beta-induced Foxp3+ Treg conversion.
|
Report
(4 results)
Research Products
(42 results)
-
-
-
-
[Journal Article] Dehydroxymethylepoxyquinomicin, a novel nuclear factor-κB inhibitor, reduces chemokines and adhesion molecule expression induced by IL-1β in human corneal fibroblasts.2015
Author(s)
Inokawa S, Watanabe T, Keino H, Sato Y, Hirakata A, Okada AA, Fukuda K, Fukushima A, Umezawa K.
-
Journal Title
Graefes Arch Clin Exp Ophthalmol.
Volume: 253
Issue: 4
Pages: 557-563
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
[Journal Article] Development and validation of new diagnostic criteria for acute retinal necrosis.2015
Author(s)
Takase H, Okada AA, Goto H, Mizuki N, Namba K, Ohguro N, Sonoda KH, Tomita M, Keino H, Kezuka T, Kubono R, Mizuuchi K, Shibuya E, Takahashi H, Yanai R, Mochizuki M
-
Journal Title
Jpn J Ophthalmol
Volume: 59(1)
Issue: 1
Pages: 14-20
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Evaluation of the long-term efficacy and safety of infliximab treatment for uveitis in Behçet's disease: a multicenter study.2014
Author(s)
Takeuchi M, Kezuka T, Sugita S, Keino H, Namba K, Kaburaki T, Maruyama K, Nakai K, Hijioka K, Shibuya E, Komae K, Hori J, Ohguro N, Sonoda KH, Mizuki N, Okada AA, Ishibashi T, Goto H, Mochizuki M.
-
Journal Title
Ophthalmology.
Volume: 121
Pages: 1877-1884
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
[Presentation] EDI-OCT of chronic and recurrent phases of VKH2015
Author(s)
Hiroshi Keino, Yasuhiko Sato,Annabelle A Okada.
Organizer
2015 International Workshops on Granulomatous Uveitis and Ocular Behcet's Disease and the (APIISG) Meeting
Place of Presentation
セブ、フィリピン
Year and Date
2015-05-15
Related Report
Int'l Joint Research / Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] 眼科2014
Author(s)
慶野博、岡田アナベルあやめ
Total Pages
4
Publisher
金原出版
Related Report
