| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In the present study, we determined whether vitreous fluid can promote the generation of Foxp3+ CD4+ T regulatory (Treg) cells in vitro. Vitreous fluid was collected from fresh porcine eyes, sterilized by filtration. Vitreous was used for culture at a final concentration of 25%. CD4+ T cells purified from pooled splenocytes from naive C57Bl/6J were stimulated with plate-bound anti-CD3 and anti-CD28 antibodies for 96h in the presence or absence of vitreous fluid. CD4+ T cells were analyzed for Foxp3 expression using flow cytometry. In some experiments, CD4+ T cells were cultured in the presence of vitreous fluid with or without TGF-beta1 or 2. Vitreous fluid alone did not increase the frequency of Foxp3+ Tregs. Although TGF-beta 1 or 2 induced Foxp3+ Tregs in vitro, the frequency of TGF-beta1/2 induced Foxp3+ Tregs increased significantly in the presence of vitreous fluid. These findings suggest that vitreous fluid may enhance TGF-beta-induced Foxp3+ Treg conversion.
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