Regulation of tissue fibrosis and natural helper cells in ocular atopy

• Project/Area Number: 26462698
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Juntendo University
• Principal Investigator: Murakami Akira 順天堂大学, 医学部, 教授 (90157743)
• Co-Investigator(Kenkyū-buntansha): 松田 彰 順天堂大学, 医学(系)研究科(研究院), 准教授 (00312348) ; 堀 寛爾 順天堂大学, 医学部, 助教 (30529227)
• Co-Investigator(Renkei-kenkyūsha): NAKAE Susumu 東京大学, 医科学研究所, 准教授 (60450409)
• Research Collaborator: ASADA Yosuke 順天堂大学, 医学部, 助教 ; IWAMOTO Satoshi 順天堂大学, 医学部, 助教 ; SUGITA Jobu 順天堂大学, 医学部, 助手
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ナチュラルヘルパー細胞 / 涙腺 / アレルギー性結膜炎 / 組織線維化 / 自然リンパ球 / アトピー性炎症 / アトピー性皮膚炎 / 緑内障 / 白内障 / 網膜剥離

Outline of Final Research Achievements

In previous study we found abundant distribution of natural helper cell (NH cell) in lacrimal gland. We investigated the roles of NH cells for the ocular surface pathology by removing extra orbital lacrimal gland tissue in mouse, and then made ragweed induced experimental allergic conjunctivitis. We found that lacrimal gland excision did not affect the number of infiltrated eosinophils. On the other hand lacrimal gland excision did induced strong corneal inflammation especially in IL-33 knockout mice. Our results suggested that NH cells and IL-33 may contribute to the homeostasis of ocular surface.

Research Products

• [Journal Article] Subcutaneous Allergic Sensitization to Protease Allergen Is Dependent on Mast Cells but Not IL-33: Distinct Mechanisms between Subcutaneous and Intranasal Routes (2016)
  • Author(s): Kamijo S, Suzuki M, Hara M, Shimura S, Ochi H, Maruyama N, Matsuda A, Saito H, Nakae S, Suto H, Ichikawa S, Ikeda S, Ogawa H, Okumura K, Takai T.
  • Journal Title: J Immunol Volume: 196 Issue: 9 Pages: 3559-3569
  • DOI: 10.4049/jimmunol.1500717
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epicutaneous allergic sensitization by cooperation between allergn protease activity and mechanical skin barrier damage in mice (2016)
  • Author(s): Shimura S, Takai T, Iida H, Maruyama N, Ochi H, Kamijo S, Nishioka I, Hara M, Matsuda A, Saito H, Nakae S, Ogawa H, Okumura K, Ikeda S.
  • Journal Title: J Invest Dermatol Volume: 136 Issue: 7 Pages: 1408-1417
  • DOI: 10.1016/j.jid.2016.02.810
  • Peer Reviewed
• [Journal Article] The roles of epithelial cell-derived type 2 initiating cytokines in experimental allergic conjunctivitis. (2015)
  • Author(s): Asada Y, Nakae S, Ishida W, Hori K, Sugita J et al.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 56 Issue: 9 Pages: 5194-5202
  • DOI: 10.1167/iovs.15-16563
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] アトピー性疾患の組織線維化 (2017)
  • Author(s): 松田 彰
  • Organizer: 第１２１回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2017-04-06
  • Invited
• [Presentation] Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis. (2014)
  • Author(s): Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae
  • Organizer: Annual meeting for the Association of Research in Vision and Ophthalmology
  • Place of Presentation: Orlando,FL,USA
  • Year and Date: 2014-05-04 – 2014-05-08