Elucidation of molecular mechanism of corneal epithelial stem cells using lineage tracing analysis
| Project/Area Number |
26462702
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine (2015-2016)
Doshisha University (2014) |
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Principal Investigator |
NAKAMURA TAKAHIRO 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30411078)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 角膜上皮 / 上皮幹細胞 / 細胞系譜解析 / Lrig1 / 創傷治癒 |
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| Outline of Final Research Achievements |
In order to investigate the mechanism of the homeostatic maintenance of corneal epithelial stem cells, we successfully generated leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) knock-in mice. Using those mice, we found that LRIG1(+) corneal epithelial stem cells are not only located in the limbal region, but also in the peripheral and central regions. Using long-term lineage tracing, we verified that quiescent corneal epithelial stem cells do exist in the limbal region. While our novel findings require further detailed examination, they may lead to a groundbreaking reconsideration of the corneal-epithelial stem-cell concept.
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Report
(4 results)
Research Products
(6 results)
