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Evaluation of the in vivo re-cellularization of the decellularized trachea in a rat model of orthotopic tracheal transplantation

Research Project

Project/Area Number 26462707
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatric surgery
Research InstitutionResearch Institute, Osaka Medical Center for Maternal and Child Health

Principal Investigator

Usui Noriaki  地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, 小児外科主任部長 (30273626)

Co-Investigator(Kenkyū-buntansha) 梅田 聡  大阪大学, 医学部附属病院, 医員 (60715176)
奥山 宏臣  大阪大学, 医学系研究科, 教授 (30252670)
奈良 啓悟  地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, 小児外科・副部長 (00432477)
上原 秀一郎  地方独立行政法人大阪市民病院機構大阪市立総合医療センター(臨床研究センター), 臨床研究センター, 医長 (00448060)
上野 豪久  大阪大学, 医学系研究科, 講師 (10456957)
田附 裕子  大阪大学, 医学系研究科, 准教授 (10397698)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords気管軟骨 / 再生医療 / 脱細胞化
Outline of Final Research Achievements

Aim: We investigated the in vivo re-cellularization of the decellularized trachea.
Materials & Methods: Nine tracheas of adult Sprague-Dawley rats were harvested and decellularized. After histological and mechanical examination of these scaffolds, the decellularized tracheas were implanted orthotopically (n=9). After 4 weeks of implantation, endoscopic evaluation of the tracheas was performed, followed by resection and histological evaluation.
Results: Complete removal of cell components after 9 cycles of decellularization was verified. The elastic intensity of the decellularized trachea corresponded to one sixth of the native trachea. Of the nine rats which received decellularized tracheal transplantation, two rats died due to graft obstruction. Consequently, survival rate after 4 weeks of implantation was 78%. Histological examination revealed that ciliated columnar epithelium was regenerated into the luminal surface of the scaffold, although a cartilage regeneration was not observed.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Enhanced Pulmonary Vascular and Alveolar Development via Prenatal Administration of a Slow-release Synthetic Prostacyclin Agonist in Rat Fetal Lung Hypoplasia.2016

    • Author(s)
      Satoshi Umeda, Shigeru Miyagawa, Satsuki Fukushima, Noriko Oda, Atsuhiro Saito, Yoshiki Sakai, Yoshiki Sawa, Hiroomi Okuyama.
    • Journal Title

      PLoS One

      Volume: 11(8) Issue: 8 Pages: e0161334-e0161334

    • DOI

      10.1371/journal.pone.0161334

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ラット同所性気管移植モデルを用いた脱細胞化気管の生体内における再細胞化の検討2016

    • Author(s)
      梅田 聡他
    • Organizer
      第53回日本小児外科学会学術集会
    • Place of Presentation
      ヒルトン福岡シーホーク
    • Year and Date
      2016-05-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット同所性気管移植モデルを用いた脱細胞化気管の生体内における再細胞化の検討2016

    • Author(s)
      梅田 聡
    • Organizer
      第53回日本小児外科学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-05-24
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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