Regeneration of diaphragm using biosheet

• Project/Area Number: 26462712
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Saitama Medical University
• Principal Investigator: Keisuke Suzuki 埼玉医科大学, 医学部, 客員講師 (50724887)
• Co-Investigator(Kenkyū-buntansha): 寺脇 幹 埼玉医科大学, 医学部, 講師 (00372384) ; 古村 眞 埼玉医科大学, 医学部, 教授 (10422289) ; 中山 泰秀 国立研究開発法人国立循環器病研究センター, その他, 研究員 (50250262) ; 佐竹 亮介 埼玉医科大学, 医学部, 客員講師 (70597525) ; 小高 哲郎 埼玉医科大学, 医学部, 講師 (80442961)
• Research Collaborator: KOMURA Hiroko 東京大学, 医学部, 登録研究員
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 横隔膜ヘルニア / 横隔膜 / 再生 / 筋肉 / biosheet / 先天性横隔膜ヘルニア / 横隔膜再生

Outline of Final Research Achievements

We evaluated the efficacy of biosheet for the regeneration and repair of diaphragm in a rabbit model.　Biosheets were prepared by embedding silicon plates in dorsal subcutaneous pouches of rabbits. Defects in the left diaphragm were created in rabbits. Autologous biosheets were transplanted into the diaphragmatic defects. Three months after transplantation of biosheets, herniation of liver was observed in 21% of rabbits, which was less often than prosthetic patches. Biosheets before transplantation had equivalent burst strength as native diaphragm. Their strength was significantly increased after transplantation. Regeneration of muscular tissue was confirmed by immunohistochemical staining with desmin and Sr-1. We confirmed that biosheets can be useful material for diaphragmatic repair and engineering diaphragmatic muscular tissue.

Research Products

• [Journal Article] Engineering and repair of diaphragm using biosheet (a collagenous connective tissue membrane) in rabbits (2018)
  • Author(s): Keisuke Suzuki, Makoto Komura, Kan Terawaki, Tetsuro Kodaka, Takumi Gohara, Hiroko Komura, Yasuhide Nakayama
  • Journal Title: Journal of Pediatric Surgery Volume: 53 Pages: 330-334
  • Peer Reviewed
• [Presentation] Evaluation of diaphragmatic engineering using biosheet, a collagenous connective tissue membrane to repair congenital diaphragmatic hernia in rabbit (2017)
  • Author(s): Keisuke Suzuki, Makoto Komura, Kan Terawaki, Tetsuro Kodaka, Takumi Gohara, Hiroko Komura, Yasuhide Nakayama
  • Organizer: BAPS Congress 2017
  • Int'l Joint Research
• [Presentation] バイオシートを用いた先天性横隔膜ヘルニアに対する横隔膜の修復と再生の研究 (2016)
  • Author(s): 鈴木 啓介
  • Organizer: 第53回日本小児外科学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2016-05-24
• [Presentation] バイオシートを用いた先天性横隔膜ヘルニアに対する横隔膜の修復と再生の研究 (2016)
  • Author(s): 鈴木啓介
  • Organizer: 第53回日本小児外科学会学術集会
  • Place of Presentation: 福岡市、ヒルトンシーホーク
  • Year and Date: 2016-05-24
• [Presentation] Repair and regeneration of diaphragm using biosheets (2016)
  • Author(s): 鈴木啓介
  • Organizer: The 49th annual meeting of the Pacific Association of Pediatric Surgeons
  • Place of Presentation: Hawii, USA
  • Year and Date: 2016-04-25
  • Int'l Joint Research
• [Presentation] Repair and regeneration of diaphragm using biosheets for congenital diaphragmatic hernia in a rabbit model (2016)
  • Author(s): Keisuke Suzuki
  • Organizer: PAPS 2016
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2016-04-24
  • Int'l Joint Research