Elucidation of the pathogenesis of dyfecation/dysuria and development of novel treatment for spina bifida using animal model.

• Project/Area Number: 26462715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Juntendo University
• Principal Investigator: YAMATAKA ATSUYUKI 順天堂大学, 医学部, 教授 (40200703)
• Co-Investigator(Kenkyū-buntansha): 宮原 克 順天堂大学, 医学(系)研究科(研究院), 研究技師 (00420844)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 二分脊椎症 / 鎖肛 / 腸管神経系 / セマフォリン３A / 虚血再灌流障害 / セマフォリン3A / 排便機能障害 / モデルマウス / 神経栄養因子 / 神経堤細胞

Outline of Final Research Achievements

This study demonstrated that the abnormality of distribution of enteric nerve system (ENS) and the expression change of GDNF was observed in the intestine of the mouse model of spina bifida. In addition, in the intestinal ischemic reperfusion experiment to detect the related factors to the restoration of ENS, we obtained the correlation between semaphorin 3A and intestinal glial cells after reperfusion.

Research Products

• [Journal Article] Pulmonary lobectomy techniques in infants and children. (2017)
  • Author(s): Yamataka A, Koga H, Ochi T, Imashimizu K, Suzuki K, Kuwatsuru R, Lane G, Nishimura K, Inada E, Suzuki K
  • Journal Title: Pediatr Surg Int. Volume: 33(4) Issue: 4 Pages: 483-495
  • DOI: 10.1007/s00383-016-4052-5
  • Peer Reviewed
• [Journal Article] Semaphorin 3A expression following intestinal ischemia/reperfusion injury in Sox10-Venus mice. (2017)
  • Author(s): Takeda M, Miyahara K, Okawada M, Akazawa C, Lane GJ, Yamataka A.
  • Journal Title: Pediatr Surg Int. Volume: 33(3) Issue: 3 Pages: 383-388
  • DOI: 10.1007/s00383-016-4039-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Surgical management of recto-prostatic and recto-bulbar anorectal malformations (2016)
  • Author(s): Yazaki Y, Koga H, Ochi T, Okawada M, Doi T, Lane GJ, Yamataka A
  • Journal Title: Pediatr Surg Int. Volume: 32(10) Issue: 10 Pages: 939-44
  • DOI: 10.1007/s00383-016-3948-4
  • Peer Reviewed
• [Journal Article] Altered differentiation of enteric neural crest-derived cells from endothelin receptor-B null mouse model of Hirschsprung's disease. (2016)
  • Author(s): Fujiwara N, Miyahara K, Nakazawa-Tanaka N, Akazawa C, Yamataka A.
  • Journal Title: Pediatr Surg Int. Volume: 32(12) Pages: 1095-1101
  • Peer Reviewed
• [Journal Article] Three- and four-dimensional analysis of altered behavior of enteric neural crest derived cells in the Hirschsprung's disease mouse model (2016)
  • Author(s): Nakazawa-Tanaka N, Miyahara K, Fujiwara N, Urao M, Akazawa C, Yamataka A
  • Journal Title: Pediatr Surg Int Volume: 32 Issue: 1 Pages: 390-407
  • DOI: 10.1007/s00383-015-3806-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pediatric Surgery (2015)
  • Author(s): wanaka T, Yamataka A, Uemura S, Okuyama H, Segawa O, Nio M, Yoshizawa J, Yagi M, Ieiri S, Uchida H, Koga H, Sato M, Soh H, Take H, Hirose R, Fukuzawa H, Mizuno M, Watanabe T
  • Journal Title: Asian J Endosc Surg Volume: 8 Issue: 4 Pages: 390-407
  • DOI: 10.1111/ases.12263
  • Peer Reviewed
• [Journal Article] Vaginal anomalies and atresia associated with imperforate anus: diagnosis and surgical management. (2015)
  • Author(s): Pandya KA, Koga H, Okawada M, Coran AG, Yamataka A, Teitelbaum DH
  • Journal Title: Journal of Pediatric Surgery Volume: 50 Pages: 431-7
  • Peer Reviewed
• [Journal Article] Comparison of outcomes between laparoscopy-assisted and posterior sagittal anorectoplasties for male imperforate anus with recto-bulbar fistula. (2014)
  • Author(s): Koga H, Ochi T, Okawada M, Doi T, Lane GJ, Yamataka A.
  • Journal Title: Journal of Pediatric Surgery Volume: 49 Pages: 1815-7
  • Peer Reviewed
• [Presentation] Semaphorin 3A expression following intestinal ischemia/reperfusion injury in Sox10-Venus mice. (2016)
  • Author(s): Takeda M, Miyahara K, Okawada M, Akazawa C, Lane GJ, Yamataka A.
  • Organizer: The 29th International Symposium on Paediatric Surgical Research
  • Place of Presentation: Frankfurt, Germany
  • Year and Date: 2016-09-08
  • Int'l Joint Research
• [Presentation] 外科道に魅せられて： 順天堂の経験 (2016)
  • Author(s): 山高篤行
  • Organizer: 第34回うつくしま泌尿器科研究会/第8回福島泌尿器手術手技研究会
  • Place of Presentation: 福島県郡山市
  • Year and Date: 2016-06-25
• [Presentation] Semaphorin 3A expression upregulated in the EDNRB(-/-) mouse model of Hirschsprung's disease. (2016)
  • Author(s): Fujiwara N, Miyahara K, Nakasawa-Tanaka N, Akazawa C,Yamatama A
  • Organizer: 17th European Paediatric Surgeons Association's Annual Congress
  • Place of Presentation: Milan, Italy
  • Year and Date: 2016-06-15
  • Int'l Joint Research
• [Presentation] Increased expression of Semaphorin3A in the Endothelin-B null mouse model of Hirschsprung's disease. (2016)
  • Author(s): Fujiwara N, Miyahara K, Nakasawa-Tanaka N, Akazawa C,Yamatama A
  • Organizer: 23rd International Meeting of the Pediatric Colorectal Club
  • Place of Presentation: Prague, Chez republic
  • Year and Date: 2016-06-14
  • Int'l Joint Research
• [Presentation] Overexpression of aquaporin 4 and the effeciveness of goreisan on the cystic remnants in a case of cervical ｌymphangioma (2015)
  • Author(s): Doi T, Fujiwara N, Miyahara K, Okawada M, Miyano G, Koga H, Yamataka A
  • Organizer: The 28th International Symposium on Paediatric Surgical Research
  • Place of Presentation: アイルランド共和国ダブリン市
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Three- and four-dimensional analysis of altered behavior of enteric neural crest derived cells in the Hirschsprung's disease mouse model (2015)
  • Author(s): Nakazawa-Tanaka N, Miyahara K, Fujiwara N, Urao M, Akazawa C, Yamataka A
  • Organizer: The 28th International Symposium on Paediatric Surgical Research
  • Place of Presentation: アイルランド共和国ダブリン市
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] Thracoscopic fissureless lobectomy in a child with CPAM, a first report (2015)
  • Author(s): Yamataka A
  • Organizer: The 48th Pacific Association of Pediatric Surgeons
  • Place of Presentation: 大韓民国済州島
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] Laparoscopic portoenterostomy for a patient with prenatally detected cystic biliary atresia (2015)
  • Author(s): Koga H, Yamataka A
  • Organizer: The 48th Pacific Association of Pediatric Surgeons
  • Place of Presentation: 大韓民国済州島
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] 神経堤細胞イメージングマウスを用いたレチノイン酸誘導直腸肛門奇形マウスの病態解析 (2014)
  • Author(s): 宮原 克, 鈴木 亮太, 村上 寛, 土井 崇, 赤澤 智宏, 山高 篤行
  • Organizer: 第51回日本小児外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-08 – 2014-05-10