| Project/Area Number |
26462717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤原 恭子 日本大学, 医学部, 助教 (40595708)
越永 從道 日本大学, 医学部, 教授 (70205376)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 小児腫瘍学 / 肝芽腫 / 腎芽腫 / 機能解析 / メチル化 / DNAメチル化 |
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| Outline of Final Research Achievements |
To newly identify genes involved in the development of hepatblastoma and neproblastoma, we analyzed the methylation status of the candidate genes in liver and kidney of mice at different developmental stages. In this analysis, we found that methylation levels of ZSCAN10、SlC16A5 were significantly higher in liver of elder mice. However, there was no significant difference in the expression levels of these genes between hepatoblastoma cells and liver tissues, and between neproblastoma and normal kidney tissues. In addition, no aberrant expression of both genes was observed in the surgical specimens of hepatoblastoma and neproblastoma. We then conducted the functional analysis of TFAP2E in heaptoblastoma. It this analysis TFAP2E silenced hepatoblastoma cells showed higher viability compared to that of control cells. On the other hand, forced expression of TFAP2E resulted in reduced cell viability. These results indicate that TFAP2E has a tumor suppressive function in hepatoblastoma.
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