| Project/Area Number |
26462718
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
杉藤 公信 日本大学, 医学部, 助教 (10328750)
藤原 恭子 日本大学, 医学部, 助教 (40595708)
古屋 武史 日本大学, 医学部, 助手 (20568539)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 小児腫瘍学 / 肝芽腫 / 腎芽腫 / 細胞増殖能 / 機能解析 / メチル化 / DNAメチル化 |
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| Outline of Final Research Achievements |
Current study was performed to newly identify tumor related genes and investigate their possibility as noble therapeutic targets in nephroblastoma and hepatoblastoma. First, we screened the candidate genes aberrantly methylated and expressed in mice skin cancer, which were induced by two step chemical carcinogenesis. Among those candidate genes, we found that the expression level of Zygote Arrest 1 (ZAR-1) was significantly suppressed in nephroblastoma and hepatoblastoma cell lines compared to normal tissues. To elucidate the function of ZAR1, we silenced this gene in these cell lines by using siRNA, however no obvious difference was observed between ZAR1 silenced cells and controls. We then conducted the functional analysis of Neuropilin 1 (NRP1) in hepatoblastoma. It was clearly shown that NRP1 silenced hepatoblastoma cells showed lower growth rate and smaller colony size compared to those in control cells. This result indicate that NRP1 has a oncogenic function in hepatoblastoma.
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