The effect of stretch stimuli on human skin equivalents

Research Project

Project/Area Number	26462732
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plastic surgery
Research Institution	Kawasaki Medical School
Principal Investigator	Hasegawa Kenjiro 川崎医科大学, 医学部, 教授 (90197674)
Co-Investigator(Kenkyū-buntansha)	成瀬恵治岡山大学, 医歯薬学総合研究科, 教授 (40252233) 徳山英二郎岡山大学, 大学病院, 助教 (90379785)
Research Collaborator	TAKAHASHI Ken 岡山大学, 医歯薬学総合研究科システム生理学, 助教 (50432258)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	メカノメディスン / human skin equivalent / 伸展培養 / 基底膜 / 細胞間interaction / 3次元全層培養皮膚
Outline of Final Research Achievements	In this study, we developed systems that enable application of stretch stimuli to HSEs during formation. Consequently, we found that in the ST group, the epidermal layer is thicker than NST group. Furthermore, synthesis of basement membrane proteins and deposition in the basal layer are increased; therefore, a more developed basement membrane is formed. Further research using this system may elucidate effects of stretching on skin properties and wound healing. In addition, application to an in vitro model of a hypertrophic scar is also expected.