Elucidation of inflammatory mechanisms by platelet-derived microRNA after cardiac surgery with cardiopulmonary bypass
| Project/Area Number |
26462761
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Emergency medicine
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
佐和 貞治 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10206013)
石井 祥代 京都府立医科大学, 医学部附属病院, 研究員 (40457958)
溝部 俊樹 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50239266)
小川 覚 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50636131)
中山 力恒 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90568198)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 遺伝子治療 |
|---|
| Outline of Final Research Achievements |
The mechanism of platelet dysfunction after cardiopulmonary bypass (CPB) is largely unknown. We therefore evaluated time-dependent changes in blood intra-platelet Bax (a pro-apoptotic molecule) concentrations and platelet dysfunction. Platelets were found to contain abundant RNAs, including miRNAs, and mRNA splicing machinery, and exhibit a protein synthesis function. We hypothesized that the expression levels of several miRNAs significantly change during CPB, and the mRNA and protein expression level alterations triggered by these miRNA changes lead to platelet dysfunction after CPB.
CPB increases platelet Bax expression, which contributes to reduced platelet-surface GPIb expression and thrombin-induced platelet calcium changes. Next generation sequencing technology revealed that the expression levels of several miRNAs in circulating platelets significantly changed during CPB. These changes in platelet signalling might contribute to platelet dysfunction after CPB.
|
Report
(5 results)
Research Products
(29 results)
-
-
[Journal Article] Potential contribution of erythrocyte microRNA to secondary erythrocytosis and thrombocytopenia in congenital heart disease2018
Author(s)
Nobuhiro Mukai, Yoshinobu Nakayama, Satoshi, Murakami, Toshihito Tanahashi, Daniel Sessler, Sachiyo Ishii, Satoru Ogawa, Natsuko Tokuhira, Toshiki Mizobe, Teiji Sawa, , Yasufumi Nakajima
-
Journal Title
Pediatric Research (e-pub ahead of print)
Volume: -
Issue: 4
Pages: 866-873
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
[Journal Article] Flow-dynamics assessment of mitral-valve surgery by intraoperative vector flow mapping2017
Author(s)
Akiyama K, Nakamura N, Itatani K, Naito Y, Kinoshita M, Shimizu M, Hamaoka S, Kato H, Yasumoto H, Nakajima Y, Mizobe T, Numata S, Yaku H, Sawa T.
-
Journal Title
Int Cardiovasc Thorac Surg
Volume: in press
Issue: 6
Pages: 869-875
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Vector flow mapping analysis of left ventricular energetic performance in healthy adult volunteers2017
Author(s)
Akiyama K, Maeda S, Matsuyama T, Kainuma A, Ishii M, Naito Y, Kinoshita M, Hamaoka S, Kato H, Nakajima Y, Nakamura N, Itatani K, Sawa T
-
Journal Title
BMC Cardiovascular Disorders
Volume: 17
Issue: 1
Pages: 21-21
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 周術期止血凝固療法2016
Author(s)
中嶋康文
Organizer
城南周術期循環管理フォーラム
Place of Presentation
東京 昭和大学
Year and Date
2016-04-23
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
