| Project/Area Number |
26462762
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
Ozawa Toshiyuki 大阪市立大学, 大学院医学研究科, 講師 (50570602)
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| Co-Investigator(Kenkyū-buntansha) |
粟津 邦男 大阪大学, 工学研究科, 教授 (30324817)
鶴田 大輔 大阪市立大学, 大学院医学研究科, 教授 (90382043)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光線力学療法 / 細菌 / 皮膚潰瘍 / 多剤耐性菌 / MRSA / 緑膿菌 |
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| Outline of Final Research Achievements |
Photodynamic therapy (PDT) is believed to have the potential to be a non-antibiotic treatment for infections. 5-Aminolevulinic acid (ALA), which works as a photosensitizer after it is metabolized protoporphyrin IX (PpIX) in heme synthetic pathway, is used for PDT. Thus far, the in vivo effectiveness of PDT using ALA against PA is still unknown. In this study, we investigated it both in vitro and in vivo. The results revealed that although PDT with ALA alone does not show a bactericidal effect against PA, PDT with both ALA and EDTA-2Na does in in vitro experiments, because PpIX has increased in PA in the latter. In in vivo experiments, a wound was healed faster in PA-infected mice using PDT with both EDTA-2Na and ALA than non-PDT. These results suggest that PDT with EDTA-2Na and ALA has a potential novel treatment option for PA-infected wound.
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