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Development of bone regeneration strategies by controlling the epigenetic state of bone lining cells.

Research Project

Project/Area Number 26462785
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionHiroshima University

Principal Investigator

YOSHIOKA HIROTAKA  広島大学, 医歯薬保健学研究院(歯), 助教 (50523411)

Co-Investigator(Kenkyū-buntansha) 吉子 裕二  広島大学, 医歯薬保健学研究院(歯), 教授 (20263709)
Co-Investigator(Renkei-kenkyūsha) NIIDA Shunpei  国立長寿医療研究センター, メディカルゲノムセンター, センター長 (10137630)
Research Collaborator MINAMIZAKI Tomoko  
TAKEI Yuichiro  
HIYAMA Shinji  
KAGAWA Kazuko  
OKITA Saki  
NAKANO Masashi  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨代謝 / エピジェネティクス / Bone lining cells / 骨形成 / 骨芽細胞
Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular basis of cell fate decisions in the bone and to identify new bone formation-promoting factors that control reactivation of bone lining cells. Venus-positive cells were isolated from transgenic mice that express yellow fluorescence protein Venus under the control of bone specific promoter and subjected single-cell RNA-sequencing to comprehensively profile the mRNA populations. All cells expressed osteoblast markers, and some cells showed a characteristic gene expression patterns that observed in bone lining cells. In addition, some cells expressed the genes that crucial for adipocyte differentiation. Thus, Venus-positive cells appear to comprise a heterogeneous cell population that may underlie the diversity in transcriptional profiles.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Comparative proteome analysis of wild-type and klotho-knockout mouse kidneys using a combination of MALDI-IMS and LC-MS/MS.2017

    • Author(s)
      Fujino Y, Minamizaki T, Hayashi I, Kawakami A, Miyaji T, Sakurai K, Yoshioka H, Kozai K, Okada M, Yoshiko Y.
    • Journal Title

      Proteomics Clin. Appl.

      Volume: 8 Issue: 7-8 Pages: 7-8

    • DOI

      10.1002/prca.201600095

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry.2016

    • Author(s)
      Fujino Y, Minamizaki T, Yoshioka H, Okada M, Yoshiko Y
    • Journal Title

      Bone Reports

      Volume: 5 Pages: 280-285

    • DOI

      10.1016/j.bonr.2016.09.004

    • NAID

      120006824051

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The dynamics of DNA methylation and hydroxymethylation during amelogenesis.2015

    • Author(s)
      Yoshioka H, Minamizaki T, Yoshiko Y.
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 144 Issue: 5 Pages: 471-478

    • DOI

      10.1007/s00418-015-1353-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 骨芽細胞の多様性と脂肪細胞への分化転換2016

    • Author(s)
      沖田紗季,吉岡広陽,南崎朋子,谷本幸太郎,吉子裕二
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Histomorphometric analysis of Klotho-deficient mice by nano-CT.2016

    • Author(s)
      Ahmed F,Minamizaki T,Yoshioka H,Yoshiko Y
    • Organizer
      日本解剖学会第71回中国・四国支部学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] シングルセルRNA-seqによる骨芽細胞の多様性の解析2016

    • Author(s)
      沖田紗季,吉岡広陽,南崎朋子,谷本幸太郎,吉子裕二
    • Organizer
      日本解剖学会第71回中国・四国支部学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] Osteoblasts inhibit osteoclast formation by targeting Prdm1 via the mechanism underlying matrix vesicle-mediated transfer of miR-125b.2016

    • Author(s)
      Irie Y, Minamizaki T, Ahmed F, Nakao Y, Yoshioka H, Tanimoto K, Kozai K, Yoshiko Y
    • Organizer
      The American Society of Bone and Mineral Research 2016 Annual Meeting
    • Place of Presentation
      Atlanta, GA, USA
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Matrix vesicle miR-125b suppress osteoclast formation by targeting Prdm1.2016

    • Author(s)
      Irie Y, Minamizaki T, Nakao Y, Ahmed F, Yoshioka H, Kozai K, Tanimoto K, Yoshiko Y
    • Organizer
      Annual Meetings of the Endocrine Society of Australia and Society for Reproductive Biology and Australia and New Zealand Bone and Mineral Society 2016
    • Place of Presentation
      Queensland, Australia
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 骨芽細胞は基質小胞を介してmiR-125bを輸送し,Prdm1を標的として破骨細胞の形成を抑制する2016

    • Author(s)
      入江泰正,南崎朋子,中尾裕子,Faisal Ahmed,吉岡広陽,香西克之,竹井悠一郎,吉子裕二
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] MicroRNA-125b in bone matrix plays a crucial role in osteoblast-osteoclast communication.2016

    • Author(s)
      Minamizaki T, Takei Y, Nakao Y, Irie Y, Yoshioka H, Tanimoto K, Kozai K, Yoshiko Y
    • Organizer
      43rd Annual European Calcified Tissue Society Congress
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2016-05-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Novel isoform of FGFR2 Modulates FGF10-FGFR Signaling in Osteochondrogenesis.2015

    • Author(s)
      Yoshioka H, Kagawa K, Okita S, Kuremoto K, Takei Y, Minamizaki T, Tanimoto K, Tsuga K, Yoshiko Y
    • Organizer
      6th Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bone-derived miRNA as a mediator of cell-cell communication.2015

    • Author(s)
      Takei Y, Minamizaki T, Yoshioka H, Yoshiko Y
    • Organizer
      6th Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Newly Identified FGFR2 Isoform Modulates FGF10-FGFR Signaling During Osteochondrogenesis.2015

    • Author(s)
      Kagawa K, Yoshioka H, Okita S, Kuremoto K, Takei Y, Minamizaki T, Tanimoto K, Tsuga K, Yoshiko Y
    • Organizer
      The American Society for Bone and Mineral Research 2015 Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Matrix Vesicles Mediate the Cell-to-Cell Transmission of MicroRNA-125b as an Inhibitor of Osteoclastic Bone Resorption.2015

    • Author(s)
      Takei Y, Nakao Y, Minamizaki T, Irie Y, Ahmed F, Yoshioka H, Niida S, Tanimoto K, Yoshiko Y
    • Organizer
      The American Society for Bone and Mineral Research 2015 Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] MicroRNAs Involved in Bone Metabolism Are Transported into Matrix Vesicles during Bone Formation.2015

    • Author(s)
      Nakao Y, Takei Y, Minamizaki T, Yoshioka H, Ahmed F, Tanimoto K, Niida S, Yoshiko Y
    • Organizer
      The American Society for Bone and Mineral Research 2015 Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2018-03-22  

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