Development of bone regeneration strategies by controlling the epigenetic state of bone lining cells.
| Project/Area Number |
26462785
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉子 裕二 広島大学, 医歯薬保健学研究院(歯), 教授 (20263709)
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| Co-Investigator(Renkei-kenkyūsha) |
NIIDA Shunpei 国立長寿医療研究センター, メディカルゲノムセンター, センター長 (10137630)
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| Research Collaborator |
MINAMIZAKI Tomoko
TAKEI Yuichiro
HIYAMA Shinji
KAGAWA Kazuko
OKITA Saki
NAKANO Masashi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨代謝 / エピジェネティクス / Bone lining cells / 骨形成 / 骨芽細胞 |
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the molecular basis of cell fate decisions in the bone and to identify new bone formation-promoting factors that control reactivation of bone lining cells. Venus-positive cells were isolated from transgenic mice that express yellow fluorescence protein Venus under the control of bone specific promoter and subjected single-cell RNA-sequencing to comprehensively profile the mRNA populations. All cells expressed osteoblast markers, and some cells showed a characteristic gene expression patterns that observed in bone lining cells. In addition, some cells expressed the genes that crucial for adipocyte differentiation. Thus, Venus-positive cells appear to comprise a heterogeneous cell population that may underlie the diversity in transcriptional profiles.
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Report
(4 results)
Research Products
(15 results)
