• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel anticancer therapy targeting specific genes for squamous cell carcinoma

Research Project

Project/Area Number 26462788
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

Wada Hiroko  九州大学, 歯学研究院, 助教 (70380706)

Co-Investigator(Kenkyū-buntansha) 清島 保  九州大学, 歯学研究院, 教授 (20264054)
永田 健吾  九州大学, 歯学研究院, 助教 (90189134)
藤原 弘明  九州大学, 歯学研究院, 助教 (50634200)
藤井 慎介  九州大学, 歯学研究院, 助教 (60452786)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords扁平上皮癌 / アポトーシス
Outline of Final Research Achievements

Deleted in colorectal cancer (DCC) is a netrin-1 receptor that is involved in axon guidance and cell survival. DCC induces apoptosis when its ligand, Netrin-1, is absent.
In this study, we investigated to analyze the inducible apoptosis mechanism by the overexpression of ΔDCC lacking Netrin-1 binding site in the carcinoma cells, and in the carcinoma tissue implanted into nude mice. The proliferation rates of ΔDCC-overexpressing cells were significantly lower than those of controls in vitro. The size of the carcinoma tissue was decreased and the number of TUNEL-positive cells was increased in ΔDCC vector injection group in vivo. These results suggested that ΔDCC overexpression may induce apoptosis in vitro and in vivo. We supposed the possibility to develop a novel method of therapy against oral squamous cell carcinoma by using a new ΔDCC vector containing the up-regulatory elements for the transcription of genes that specifically express in squamous cell carcinoma.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (13 results)

  • [Journal Article] GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas2017

    • Author(s)
      Mikami Y, Fujii S, Nagata K, Wada H, Hasegawa K, Abe M, Yoshimoto RU, Kawano S, Nakamura S, Kiyoshima T
    • Journal Title

      J Cancer Res Clin Oncol

      Volume: 印刷中 Issue: 8 Pages: 1381-1393

    • DOI

      10.1007/s00432-017-2398-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thymosin beta 4 is associated with RUNX2 expression via the Smad and Akt signaling pathways in mouse dental epithelial cells2015

    • Author(s)
      Someya H., Fujiwara H.†, Nagata K., Wada H., Hasegawa K., Mikami Y., Jinno A., Sakai H., Koyano K. and Kiyoshima T. (†: Corresponding author)
    • Journal Title

      Int. J. Mol. Med.

      Volume: 35 Issue: 5 Pages: 1169-1178

    • DOI

      10.3892/ijmm.2015.2118

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased expression of interleukin-6 predicts poor response to chemoradiotherapy and unfavorable prognosis in oral squamous cell carcinoma.2015

    • Author(s)
      Jinno T, Kawano S, Maruse Y, Matsubara R, Goto Y, Sakamoto T, Hashiguchi Y, Kaneko N, Tanaka H, Kitamura R, Toyoshima T, Jinno A, Moriyama M, Oobu K, Kiyoshima T, Nakamura S.
    • Journal Title

      Oncol Rep

      Volume: 33 Issue: 5 Pages: 2161-2168

    • DOI

      10.3892/or.2015.3838

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Itm2a expression in the developing mouse first lower molar, and the subcellular localization of itm2a in mouse dental epithelial cells.2014

    • Author(s)
      Kihara M., Kiyoshima T., Nagata K., Wada H., Fujiwara H., Hasegawa K., Someya H., Takahashi I. and Sakai H.
    • Journal Title

      PLoS One

      Volume: 9 (7)

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 口腔扁平上皮癌においてGli阻害(GANT61)は細胞死を誘導する2016

    • Author(s)
      三上友里恵、永田健吾、和田裕子、藤井慎介、安部みさき、吉本怜子、清島保、中村誠司
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      北海道札幌コンベンションセンター
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mandibular tumor2016

    • Author(s)
      Hiroko Wada, Aki Kinjo, Yurie, Mikami, Tsuyoshi Sugiura, Tamotsu Kiyoshima
    • Organizer
      第27回日本臨床口腔病理学会総・学術大会
    • Place of Presentation
      広島大学応仁会館
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] A case of adenosquamous carcinoma observed in the metastatic lymph node, but not in the primary lesion.2016

    • Author(s)
      Yurie Mikami, Hiroko Wada, Kazunari Oobu, Seiji Nakamura, Tamotsu Kiyoshima
    • Organizer
      第27回日本臨床口腔病理学会総・学術大会
    • Place of Presentation
      広島大学応仁会館
    • Year and Date
      2016-08-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 特異なCT像を呈した下顎骨線維-骨性病変の一例2015

    • Author(s)
      吉住潤子、和田裕子、清水真弓、碇竜也、新田秀一、清島保、堀之内康文
    • Organizer
      第48回NPO法人日本口腔科学会九州地方部会
    • Place of Presentation
      アクロス福岡(福岡)
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 硬組織形成期歯胚におけるGlucose regulated protein-78タンパクの発現局在について2015

    • Author(s)
      永田健吾、和田裕子、藤原弘明、三上友理恵、神野彰子、安倍みさき、清島 保
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 埋伏歯を含み上顎洞内に発生した限局性骨性異形成症の一例2015

    • Author(s)
      吉住潤子、上里 梓、碇 竜也、新田秀一、清水真弓、和田裕子、清島 保、堀之内康文
    • Organizer
      日本口腔外科学会・第83回九州支部学術集会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2015-06-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 右下顎骨腫瘍2015

    • Author(s)
      和田裕子、金城亜紀、山田裕一、孝橋賢一、杉浦 剛、小田義直、清島 保
    • Organizer
      第54回日本骨軟部腫瘍研究会
    • Place of Presentation
      九州大学コラボステーション(福岡)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
  • [Presentation] The expression of Netrin-1 and its receptor DCC in oral squamous cell carcinoma2015

    • Author(s)
      Furusho K., Wada H., Mikami Y. and Kiyoshima T.
    • Organizer
      Kyudai Oral Bioscience 2015 -9th International Symposium-
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-02-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Immunocytochemical detection of glucose-related protein 78 in oral squamous cell carcinoma cell lines.2015

    • Author(s)
      Abe M., Nagata K., Jinno A. and Kiyoshima T.
    • Organizer
      Kyudai Oral Bioscience 2015 -9th International Symposium-
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-02-28
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス歯胚形成におけるFacioscapulohumeral Muscular Dystrophy Region Gene 1(FRG1)の発現様式.と機能解析2014

    • Author(s)
      長谷川佳那、和田裕子、清島保
    • Organizer
      第91回九大病理研究会
    • Place of Presentation
      九州大学百年講堂(福岡)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯胚の発生・発育におけるFacioscapulohumeral Muscular Dystrophy Region Gene 1(FRG1)の発現様式.2014

    • Author(s)
      長谷川佳那、和田裕子、永田健吾、藤原弘明、染矢祐孝、神野彰子、三上友理恵、赤峰昭文、清島保
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] V-ATPase 阻害剤 Concanamycin A 抵抗性口腔扁平上皮癌細胞株に対する SAHA 併用による細胞増殖抑制効果.2014

    • Author(s)
      清島保、永田健吾、和田裕子、藤原弘明、坂井英隆
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      ANAクラウンプラザホテル広島(広島)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 非歯原性上皮細胞へのThymosin beta 4 遺伝子導入による歯原性上皮細胞への誘導.2014

    • Author(s)
      藤原弘明、清島保、永田健吾、和田裕子、坂井英隆
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      ANAクラウンプラザホテル広島(広島)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi