• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The mechanism for haem acquisition in periodontal pathogen, Prevotella intermedia.

Research Project

Project/Area Number 26462789
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionNagasaki University

Principal Investigator

NAITO Mariko  長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (20244072)

Co-Investigator(Renkei-kenkyūsha) SHOJI Mikio  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175)
SATO Keiko  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords口腔細菌学 / 歯周病原菌 / ゲノム / 遺伝子操作 / 病原因子 / 鉄獲得
Outline of Final Research Achievements

Heam acquisition is the essential for survival of periodontal pathogen, Prevotella intermedia. Our previous study suggested that the Type IX secretion system (T9SS) might translocate the essential component for heam acquisition. Here we determined the whole genome sequence and all genes of P. intermedia. Using the conserved amino-acid sequence motifs for the T9SS-translocated protein, six candidate genes were defined. By our establish site-directed mutagenesis, we succeed to generate 5 mutants. One of those mutants showed the defects of pigmentation on blood agar plates. It indicated that this gene has the essential role for heam acquisition in P. intermedia.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 歯周病原微生物のゲノム解析に基づく病原因子の解析2017

    • Author(s)
      内藤真理子
    • Journal Title

      生体の科学

      Volume: 68 Pages: 114-117

    • Related Report
      2016 Annual Research Report
  • [Journal Article] The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent finescale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat.2016

    • Author(s)
      Mariko Naito, Yoshitoshi Ogura, Takehiko Itoh, Mikio Shoji, Masaaki Okamoto, Tetsuya Hayashi, and Koji Nakayama
    • Journal Title

      DNA Research

      Volume: 23 Pages: 11-16

    • DOI

      10.1093/dnares/dsv032

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Complete genome sequencing of Prevotella intermedia and fine-scale genomic analysis.2016

    • Author(s)
      内藤真理子(代表)、小椋義俊、庄子幹郎、林哲也、中山 浩次
    • Organizer
      94th General Session and Exhibition of the IADR
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The fine-scale intra-species genomic comparison in Prevotella intermedia strains.2016

    • Author(s)
      内藤真理子(代表) 小椋義俊、林哲也、中山浩次
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府・大阪市)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Prevotella intermediaにおける菌株間のゲノム比較2015

    • Author(s)
      内藤真理子(代表) 中山 浩次
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Prevotella intermediaにおける病原遺伝子特異的変異株作製2014

    • Author(s)
      内藤真理子(代表) 庄子 幹郎、成田 由香、中山 浩次
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi