The mechanism for haem acquisition in periodontal pathogen, Prevotella intermedia.

• Project/Area Number: 26462789
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: NAITO Mariko 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (20244072)
• Co-Investigator(Renkei-kenkyūsha): SHOJI Mikio 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175) ; SATO Keiko 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 口腔細菌学 / 歯周病原菌 / ゲノム / 遺伝子操作 / 病原因子 / 鉄獲得

Outline of Final Research Achievements

Heam acquisition is the essential for survival of periodontal pathogen, Prevotella intermedia. Our previous study suggested that the Type IX secretion system (T9SS) might translocate the essential component for heam acquisition. Here we determined the whole genome sequence and all genes of P. intermedia. Using the conserved amino-acid sequence motifs for the T9SS-translocated protein, six candidate genes were defined. By our establish site-directed mutagenesis, we succeed to generate 5 mutants. One of those mutants showed the defects of pigmentation on blood agar plates. It indicated that this gene has the essential role for heam acquisition in P. intermedia.

Research Products

• [Journal Article] 歯周病原微生物のゲノム解析に基づく病原因子の解析 (2017)
  • Author(s): 内藤真理子
  • Journal Title: 生体の科学 Volume: 68 Pages: 114-117
• [Journal Article] The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent finescale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat. (2016)
  • Author(s): Mariko Naito, Yoshitoshi Ogura, Takehiko Itoh, Mikio Shoji, Masaaki Okamoto, Tetsuya Hayashi, and Koji Nakayama
  • Journal Title: DNA Research Volume: 23 Pages: 11-16
  • DOI: 10.1093/dnares/dsv032
  • Peer Reviewed / Open Access
• [Presentation] Complete genome sequencing of Prevotella intermedia and fine-scale genomic analysis. (2016)
  • Author(s): 内藤真理子(代表)、小椋義俊、庄子幹郎、林哲也、中山 浩次
  • Organizer: 94th General Session and Exhibition of the IADR
  • Place of Presentation: ソウル（韓国）
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] The fine-scale intra-species genomic comparison in Prevotella intermedia strains. (2016)
  • Author(s): 内藤真理子(代表) 小椋義俊、林哲也、中山浩次
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府・大阪市）
  • Year and Date: 2016-03-23
• [Presentation] Prevotella intermediaにおける菌株間のゲノム比較 (2015)
  • Author(s): 内藤真理子(代表) 中山 浩次
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] Prevotella intermediaにおける病原遺伝子特異的変異株作製 (2014)
  • Author(s): 内藤真理子(代表) 庄子 幹郎、成田 由香、中山 浩次
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27