Elucidation of angio and lymphangiogenesis mechanism by novel oral cancer-related gene

• Project/Area Number: 26462790
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Nara Medical University
• Principal Investigator: Sasahira Tomonori 奈良県立医科大学, 医学部, 講師 (90405374)
• Co-Investigator(Kenkyū-buntansha): 國安 弘基 奈良県立医科大学, 医学部, 教授 (00253055)
• Co-Investigator(Renkei-kenkyūsha): KIRITA Tadaaki 奈良県立医科大学, 医学部, 教授 (70201465)
• Research Collaborator: YAMAMOTO Kazuhiko ; Bhawal Ujjal
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 口腔癌 / 新規マーカー / 浸潤・転移

Outline of Final Research Achievements

We found that N4BP2L1 and STOX2 are novel angiogenesis and lymmphangiogenesis d\factor in oral cancer. Expression of N4BP2L1 was regulated by the activation of miR-448. Overexpression of N4BP2L1 was significantly associated with nodal metastasis and poor prognosis in oral cancer specimens. We also revealed that MIA increased expression of STOX2 by paracrine manner in oral cancer cells. Furthermore, STOX2 expression was associated with acquisition of multidrug resistance and nodal metastasis in oral cancer. Our findings indicate that N4BP2L1 and STOX2 may be a useful diagnostic and therapeutic target in oral cancer.

Research Products

• [Journal Article] Storkhead box 2 and melanoma inhibitory activity promote oral squamous cell carcinoma progression. (2016)
  • Author(s): Sasahira T, Nishiguchi Y, Fujiwara R, Kurihara M, Kirita T, Katrin BA, Kuniyasu H.
  • Journal Title: Oncotarget Volume: vol.7 Issue: 18 Pages: 26751-64
  • DOI: 10.18632/oncotarget.8495
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A comprehensive expression analysis of the MIA gene family in malignancies: MIA gene family members are novel, useful markers of esophageal, lung, and cervical squamous cell carcinoma. (2016)
  • Author(s): Sasahira T, Kirita T, Nishiguchi Y, Kurihara M, Nakashima C, Bosserhoff AK, Kuniyasu H.
  • Journal Title: Oncotarget Volume: May 16 Issue: 21 Pages: 1-16
  • DOI: 10.18632/oncotarget.9082
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] NEDD 4 binding protein 2-like 1 promotes cancer cell invasion in oral squamous cell carcinoma. (2016)
  • Author(s): Sasahira T, Kurihara M, Nishiguchi Y, Fujiwara R, Kirita T; Kuniyasu H
  • Journal Title: Virchow Arch Volume: 469 Issue: 2 Pages: 163-172
  • DOI: 10.1007/s00428-016-1955-4
  • Peer Reviewed / Open Access
• [Journal Article] LEM domain containing 1 promotes oral squamous cell carcinoma invasion and endothelial transmigration. (2016)
  • Author(s): Sasahira T, Kurihara M, Nakashima C, Kirita T, Kuniyasu H
  • Journal Title: Br J Cancer Volume: 115 Issue: 1 Pages: 52-58
  • DOI: 10.1038/bjc.2016.167
  • Peer Reviewed / Open Access
• [Journal Article] 18F-fluoro-2-deoxyglucose-positron emission tomography for the assessment of histopathological response after preoperative chemoradiotherapy in oral squamous cell carcinoma. (2015)
  • Author(s): Shimomura H, Sasahira T, Yamanaka Y, Kurihara M, Imai Y, Tamaki S, Yamakawa N, Shirone N, Hasegawa M, Kuniyasu H, Kirita T
  • Journal Title: International Journal of Clinical Oncology Volume: 20(2) Issue: 2 Pages: 308-316
  • DOI: 10.1007/s10147-014-0711-5
  • Peer Reviewed / Open Access
• [Journal Article] Update of molecular pathobiology in oral cancer: a review. (2014)
  • Author(s): Sasahira T, Kirita T, Kuniyasu H.
  • Journal Title: International Journal of Clinical Oncology Volume: 19 Issue: 3 Pages: 431-436
  • DOI: 10.1007/s10147-014-0684-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transport and Golgi organisation protein 1 is a novel tumour progressive factor in oral squamous cell carcinoma (2014)
  • Author(s): Sasahira T, Kirita T, Yamamoto K, Ueda N, Kurihara M, Matsushima S, Bhawal UK, Bosserhoff AK, Kuniyasu H
  • Journal Title: Eur J Cancer Volume: 50 Issue: 12 Pages: 2142-2151
  • DOI: 10.1016/j.ejca.2014.05.006
  • Peer Reviewed / Open Access
• [Journal Article] HuD promotes progression of oral squamous cell carcinoma (2014)
  • Author(s): Sasahira T, Kurihara M, Yamamoto K, Ueda N, Nakashima C, Matsushima S, Bhawal UK, Kirita T, Kuniyasu H
  • Journal Title: Pathobiology Volume: 81 Issue: 4 Pages: 206-214
  • DOI: 10.1159/000366022
  • Peer Reviewed / Open Access
• [Presentation] STOX2とMIAは口腔癌の進展を促進する (2016)
  • Author(s): 笹平智則、西口由希子、中嶋千恵、栗原 都、桐田忠昭、國安弘基
  • Organizer: 第75回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] 口腔扁平上皮癌におけるmalic enzyme発現の意義 (2016)
  • Author(s): 中嶋千恵、桐田忠昭、栗原 都、松島紗弥子、バワール ウジャール、山本一彦、笹平智則、國安弘基
  • Organizer: 第75回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] 口腔扁平上皮癌におけるmalic enzymeの発現 (2015)
  • Author(s): 中嶋智恵、桐田忠昭、栗原 都、山本一彦、岩田直也、桂川広幸、笹平智則、國安弘基
  • Organizer: 第74回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 口腔扁平上皮癌におけるN4BP2L1の役割 (2015)
  • Author(s): 笹平智則、栗原 都、バワール・ウジャール、桐田忠昭、國安弘基
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30
• [Presentation] 大腸癌・口腔癌におけるアンギオテンシンⅡ2型受容体の意義 (2014)
  • Author(s): 松島紗弥子、羅奕、周林艶、佐藤清哉、千原良友、笹平智則、佐々木隆光、國安弘基
  • Organizer: 第73回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 口腔癌におけるN4BP2L1の発現 (2014)
  • Author(s): 笹平智則、栗原 都、バワール・ウジャール、桐田忠昭、國安弘基
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 口腔扁平上皮癌におけるMIA2の役割 (2014)
  • Author(s): 栗原 都、笹平智則、山本一彦、バワール・ウジャール、桐田忠昭、國安弘基
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 口腔扁平上皮癌におけるピルビン酸キナーゼ・アイソザイムの意義 (2014)
  • Author(s): 栗原 都、笹平智則、山本一彦、松島紗弥子、桐田忠昭、國安弘基
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26