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Resolution of Oral Biofilme Formation at Early Stage

Research Project

Project/Area Number 26462793
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

NAKAZAWA Futoshi  北海道医療大学, 歯学部, 教授 (60115053)

Co-Investigator(Kenkyū-buntansha) 宮川 博史  北海道医療大学, 歯学部, 講師 (30219729)
藤田 真理  北海道医療大学, 歯学部, 助教 (50405669)
Co-Investigator(Renkei-kenkyūsha) MASHIMA Izumi  日本学術振興会, 特別研究員 (60770782)
Research Collaborator CITRA F. Theodorea  北海道医療大学, 大学院歯学研究科, 大学院生
THAWEBOON Boonyanit  Mahidol University, Department of Oral Microbiology, Faculty of Dentistry, 講師
THAWEBOON Sroisiri  Mahidol University, Department of Oral Microbiology, Faculty of Dentistry, 教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords口腔バイオフィルム / Veillonella / Streptococcus / メタゲノム解析 / 口腔清掃 / 唾液の細菌叢 / rpo B / 新菌種 / Oral biofilm / Oral Veillonella / V. tobetsuensis, / periodontitis / ドラフトゲノムシークエンス / 口腔細菌学 / 口腔バイオフィルム / Oral Streptococcus / 歯周ポケット / Initial Colonizer / Early Colonizer
Outline of Final Research Achievements

Ora biofilm cause dental caries and periodotal disease. Streptcoccus and Veillonella are known to have important roles in the biofilm formation at aerly stage.This study demonstrated the following results. 1. Auto inducer from V. tobetsuensis inhibited biofilm formation of S. gordonii. 2. Draft genome of V. tobetsuensis was revealed. 3. New method for identification of all veillonella species was established. 4. Based on metagenome analysis of saliva, it was demonstrated that Streptococcus species incresed in good oral hygiene, but Veillonella species incresde in poor ora hygiene. 5. Many unknown Veillonella species inhabited in oral cavities with poor oral hygiene.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (43 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 10 results,  Acknowledgement Compliant: 13 results) Presentation (26 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Int'l Joint Research] Mahidol University/Faculty of Dentistry/Department of Oral Biology(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Mahidol University/Department of Oral Biology(タイ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Application of Dental Implants Coated with Titanium Nitride; The Experimental Study with Porphyromonas gingivalis Infection2017

    • Author(s)
      Yamazaki K, Mashima I, Nakazawa F, Nakanishi Y, Ochi M.
    • Journal Title

      International Journal of Current Microbiology and Applied Sciences

      Volume: 6 Issue: 1 Pages: 130-142

    • DOI

      10.20546/ijcmas.2017.601.017

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis2017

    • Author(s)
      Saeki A, Suzuki T, Hasebe A, Kamezaki R, Fujita M, Nakazawa F, Shibata K
    • Journal Title

      Cell Microbiol

      Volume: 19 Issue: 3 Pages: 1-12

    • DOI

      10.1111/cmi.12663

    • NAID

      120006401446

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Veillonella Species in the Tongue Biofilm by Using a Novel One-step Polymerase Chain Reaction Method2016

    • Author(s)
      Mashima I, Theodorea CF, Thaweboon B, Thaweboon S, Nakazawa F.
    • Journal Title

      PLoS ONE

      Volume: 11 Issue: 6 Pages: 1-16

    • DOI

      10.1371/journal.pone.0157516

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effect of roselle calyx extract on gingipain activity, production of inflammatory cytokines, and oral bacterial morphology.2016

    • Author(s)
      Herastuti Sulistyani, Mari Fujita, Futoshi Nakazawa
    • Journal Title

      Journal of Microbiology, Biotechnology and Food Sciences

      Volume: 6(3) Pages: 961-965

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria.2016

    • Author(s)
      Sulistyani H, Fujita M, Miyakawa H, Nakazawa F
    • Journal Title

      Asian Pacific Journal of Topical Medicine

      Volume: 9(2) Pages: 119-124

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory2016

    • Author(s)
      Kawaguchi K, Iijima M, Miyakawa H, Ohta M, Muguruma T, Endo K, Nakazawa F, Mizoguchi I
    • Journal Title

      J Biomed Mater Res B Appl Biomater

      Volume: 2017 Issue: 5 Pages: 1-6

    • DOI

      10.1002/jbm.b.33664

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The role of AI-2 like substance from Veillonella tobetsuensis.2016

    • Author(s)
      Izumi MASHIMA, Hiroshi MIYAKAWA, Futoshi NAKAZAWA
    • Journal Title

      Bacterial Adherence & Biofilm

      Volume: 29

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Monitoring the decontamination efficacy of the novel Poseidon-S disinfectant system in dental unit water lines2016

    • Author(s)
      Mari FUJITA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Journal Title

      Journal of Microbiology, Immunology and Infection

      Volume: xxx Issue: 3 Pages: 270-276

    • DOI

      10.1016/j.jmii.2015.05.006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Draft Genome Sequence of Veillonella tobetsuensis ATCC BAA-2400T Isolated from Human Tongue Biofilm2015

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Journal Title

      Genome Announcements

      Volume: 3 Issue: 4 Pages: 1-2

    • DOI

      10.1128/genomea.00808-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Distribution and Frequency of Veillonella spp. associated with Chronic Periodontal Diseases2015

    • Author(s)
      Izumi MASHIMA, Mari FUJITA, Yuko NAKATSUKA, Takashi KADO, Yasushi FURUICHI, Herastuti, SULISTYANI, Futoshi NAKAZAWA
    • Journal Title

      International Journal of Current Microbiology and Applied Sciences

      Volume: 4 Pages: 150-160

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The interaction between Streptococcus spp. and Veillonella tobetsuensis in oral biofilm formation at early stage2015

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Journal Title

      Journal of Bacteriology

      Volume: 197 Issue: 13 Pages: 2104-2111

    • DOI

      10.1128/jb.02512-14

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Distribution and Frequency of Veillonella spp. associated with Chronic Periodontal Diseases,2015

    • Author(s)
      Izumi MASHIMA, Mari FUJITA, Yuko NAKATSUKA, Takashi KADO, Yasushi FURUICHI, Herastuti, SULISTYANI, Futoshi NAKAZAWA,
    • Journal Title

      International Journal of Current Microbiology and Applied Sciences

      Volume: 4 Pages: 150-160

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Monitoring the decontamination efficacy of the novel Poseidon-S disinfectant system in dental unit water lines2015

    • Author(s)
      Mari FUJITA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Journal Title

      Journal of Microbiology, Immunology and Infection

      Volume: 未定

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The influence of oral Veillonella species on biofilm formed by Streptococcus species2014

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA,
    • Journal Title

      Anaerobe

      Volume: 28 Pages: 54-61

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Application of tea tree oil for disinfection of dental unit waterlines2014

    • Author(s)
      Izumi MASHIMA, Yukie OKA, Miku AOKI, Futoshi NAKAZAWA
    • Journal Title

      The Dental Journal of Health Sciences University of Hokkaido

      Volume: 33 Pages: 1-8

    • NAID

      120005459167

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Exploring the Microbial Community in Saliva from Children2017

    • Author(s)
      Izumi MASHIMA, Citra F. THEODOREA, Futoshi NAKAZAWA
    • Organizer
      95 General Session of International Association of Dental Research
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2017-03-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Correlation of Oral Veillonella Species with Oral Hygiene Status2017

    • Author(s)
      Citra F. THEODOREA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      95 General Session of International Association of Dental Research
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2017-03-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploring the Microbiome in Saliva from (Thai) Children2017

    • Author(s)
      Izumi Mashima, Citra F. Theodorea, Futoshi Nakazawa
    • Organizer
      日本細菌学会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] DNA sequence analysis of the novel Veillonella species isolated from saliva of Thai children2017

    • Author(s)
      Citra Fragrantia THEODOREA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      日本細菌学会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] The Distribution and Frequency of Oral Veillonella species in Saliva from Thai Children Associated with Oral Hygiene Status2016

    • Author(s)
      Citra Fragrantia THEODOREA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] タイ児童舌苔から分離されたVeillonella属新菌種の塩基配列解析2016

    • Author(s)
      眞島 いづみ、シトラ セオドレア、中澤 太
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] The Strategy for Oral Biofilm -The Best Use of Oral Veillonella2016

    • Author(s)
      Izumi Mashima, Citra Fragrantia Theodorea, Hiroshi Miyakawa, Futoshi Nakazawa
    • Organizer
      歯科基礎医学会(メインシンポジウム)
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Evaluation of structure-activity relationships in penetration of terpene compound into the preformed biofilm2016

    • Author(s)
      M. Fujita, K. Osada, H. Miyakawa, F. Nakazawa
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] APPLICATION OF TITANIUM NITRIDE FOR DENTAL IMPLANTS:A RABBIT STUDY2016

    • Author(s)
      Izumi MASHIMA, Koutaro YAMAZAKI, Futoshi NAKAZAWA, Yasuhiro NAKANISHI, Morio OCHI
    • Organizer
      Asian Academy of Osseointegretion 2016
    • Place of Presentation
      Bangkok
    • Year and Date
      2016-06-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Veillonella tobetsuensis由来AI-2の口腔バイオフィルム形成初期における影響2016

    • Author(s)
      眞島いづみ、中澤太
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター、大阪市
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Distribution and Frequency of Oral Veillonella species in Saliva from The Children in Thailand2016

    • Author(s)
      Citra Fragrantia THEODOREA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター、大阪市
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Distribution and Frequency of Oral Veillonella species in Saliva among 8 to 15-Year-old Children in Thailand2016

    • Author(s)
      Citra Fragrantia THEODOREA, Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      第34回北海道医療大学歯学会
    • Place of Presentation
      北海道医療大学サテライトキャンパス、札幌市
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 口腔Veillonella全6菌種同定One Step PCR法の開発2016

    • Author(s)
      眞島いづみ、Citra Fragrantia Theodorea,中澤 太
    • Organizer
      日本細菌学会北海道支部会
    • Place of Presentation
      札幌
    • Related Report
      2016 Annual Research Report
  • [Presentation] Distribution od Oral Veillonella species Associated with Oral Hygiene Status2016

    • Author(s)
      Citra Fragrantia Theodorea, Izumi Mashima, Futoshi Nakazawa
    • Organizer
      日本細菌学会北海道支部会
    • Place of Presentation
      札幌
    • Related Report
      2016 Annual Research Report
  • [Presentation] he Role of Autoinducers from Veillonella in the Formation of Oral Biofilms2015

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      the 7th ASM Conference on Biofilms
    • Place of Presentation
      Hyatt Regency Chicago, Chicago, IL, USA
    • Year and Date
      2015-10-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] he Roles of Oral Veillonella in Biofilm Formation2015

    • Author(s)
      Izumi MASHIMA, Hiroshi MIYAKAWA, Futoshi NAKAZAWA
    • Organizer
      Invited lecture
    • Place of Presentation
      State University at Buffalo, NY, USA
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 口腔バイオフィルム初期形成期におけるcyclic dipeptideの影響2015

    • Author(s)
      眞島いづみ、宮川博史、中澤太、
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ、新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular Biological Control Method for Oral Biofilm at Early Stage2015

    • Author(s)
      Izumi MASHIMA, Hiroshi MIYAKAWA, Futoshi NAKAZAWA
    • Organizer
      第57回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      朱鷺メッセ、新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Veillonella tobetsuensis由来Autoinducer-2様物質の役割2015

    • Author(s)
      眞島いづみ、宮川博史、中澤太
    • Organizer
      第29回日本バイオフィルム学会学術集会
    • Place of Presentation
      ホテル竹島、愛知県蒲郡市
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 口腔バイオフィルム初期形成菌によるクオラムセンシング機構の解析2015

    • Author(s)
      眞島いづみ、鎌口有秀、宮川博史、藤田真理、中澤太
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場、岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Production of autoinducers by Veillonella tobetsuensis associated with oral biofilm formation at early stage2014

    • Author(s)
      眞島いづみ、中澤太
    • Organizer
      第62回国際歯科研究学会日本部会総会・学術大会(JADR 2014)
    • Place of Presentation
      KKRホテル大阪、大阪
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Detection of Autoinducers from Veillonella tobetsuensis and Their Roles in Biofilm Formation2014

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      the 5th ASM Conference on Cell-Cell Communication in Bacteria
    • Place of Presentation
      San Antonio, Texas, USA
    • Year and Date
      2014-10-18 – 2014-10-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Veillonella tobetsuensis由来Autoinducerの検出と精製2014

    • Author(s)
      眞島いづみ、鎌口有秀、宮川博史、藤田真理、中澤太
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Bacterial communication between Veillonella and Streptococcus in biofilm formation2014

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      International Union of Microbiological Societies 2014
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Research-status Report
  • [Presentation] Autoinducer-2 from Novel Veillonella tobetsuensis Influences on Oral Biofilm2014

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA
    • Organizer
      2014 IADR Unilever Hatton Competition
    • Place of Presentation
      Cape town, South Africa.
    • Year and Date
      2014-06-24 – 2014-06-28
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] The Crucial Roles of Autoinducer-2 from V. tobetsuensis in Oral Biofilm2014

    • Author(s)
      Izumi MASHIMA, Futoshi NAKAZAWA,
    • Organizer
      2014 4th Research Week International Symposium of Oral Medicine
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2014-05-19 – 2014-05-25
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2022-02-16  

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