| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The purpose of this study is to clarify how the tissue oxygen concentration varying with the blood vessel distribution regulates ameloblasts differentiation. Experiments using transgenic mice and dental epithelial cell lines revealed that undifferentiated dental epithelial cells were under more hypoxic condition, and relied more on glycolysis to generate energy compared to differentiated ameloblasts. Further, RhoA activity and stem cell marker expression in dental epithelial cells depended on oxygen concentration. Together, these results demonstrated that tissue oxygen concentration controled the dental epithelial cell differentiation through RhoA signaling and cellular energy metabolism.
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