| Project/Area Number |
26462798
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Niigata University (2016)
Nihon University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
渡辺 信和 東京大学, 医科学研究所, 特任准教授 (10334278)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 骨芽細胞 / 脱分化 / 幹細胞 / 未分化間葉系幹細胞 / Pannexin 3 / 上皮間葉転換 / 間葉系幹細胞 / 細胞接着因子 |
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| Outline of Final Research Achievements |
We investigated the mechanism in which osteoblasts dedifferentiated and obtained pluripotency. Mesenchymal stem cells (MSCs) were differentiated to osteoblasts by osteogenic induction medium. After the MSCs differentiated to osteoblasts, they were isolated each other and re-cultured onto the cell culture dishes. The expressions of osteogenic markers in the MSCs (osteoblasts) were decreased during the re-culture, and then the re-cultured MSCs obtained pluripotency. Osteogenic differentiation of the MSCa was accompanied by the increase of several cell adhesion molecules including Pannexin3, which was decreased after the re-culture. In addition, the MSCs in which pannexine3 expression was inhibited by shRNA failed to differentiate to osteoblasts. On the basis of these results, it was suggested that cell-cell adhesion by Pannexin3 was required for osteogenic differentiation and maintenance of osteoblasts, and attenuation of the cell adhesion induced the dedifferentiation of osteoblasts.
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