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An investigation into the mechanisms of dedifferentiation and acquisition of pluripotency for osteoblasts

Research Project

Project/Area Number 26462798
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionNiigata University (2016)
Nihon University (2014-2015)

Principal Investigator

Mikami Yoshikazu  新潟大学, 医歯学系, 准教授 (80434075)

Co-Investigator(Kenkyū-buntansha) 渡辺 信和  東京大学, 医科学研究所, 特任准教授 (10334278)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords骨芽細胞 / 脱分化 / 幹細胞 / 未分化間葉系幹細胞 / Pannexin 3 / 上皮間葉転換 / 間葉系幹細胞 / 細胞接着因子
Outline of Final Research Achievements

We investigated the mechanism in which osteoblasts dedifferentiated and obtained pluripotency. Mesenchymal stem cells (MSCs) were differentiated to osteoblasts by osteogenic induction medium. After the MSCs differentiated to osteoblasts, they were isolated each other and re-cultured onto the cell culture dishes. The expressions of osteogenic markers in the MSCs (osteoblasts) were decreased during the re-culture, and then the re-cultured MSCs obtained pluripotency. Osteogenic differentiation of the MSCa was accompanied by the increase of several cell adhesion molecules including Pannexin3, which was decreased after the re-culture. In addition, the MSCs in which pannexine3 expression was inhibited by shRNA failed to differentiate to osteoblasts. On the basis of these results, it was suggested that cell-cell adhesion by Pannexin3 was required for osteogenic differentiation and maintenance of osteoblasts, and attenuation of the cell adhesion induced the dedifferentiation of osteoblasts.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion.2016

    • Author(s)
      Mikami Y, Fukushima A, Komiyama Y, Iwase T, Tsuda H, Higuchi Y, Hayakawa S, Kuyama K, Komiyama K.
    • Journal Title

      Cancer Lett.

      Volume: 379(1) Issue: 1 Pages: 84-93

    • DOI

      10.1016/j.canlet.2016.05.028

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner2016

    • Author(s)
      Mikami Y, Tsuda H, Akiyama Y, Honda M, Shimizu N, Suzuki N, Komiyama K
    • Journal Title

      J Bone Miner Metab

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteocytes up-regulate the terminal differentiation of pre-osteoblasts via gap junctions.2015

    • Author(s)
      Nishikawa Y, Akiyama Y, Yamamoto K, Kobayashi M, Watanabe E, Watanabe N, Shimizu N, Mikami Y, Komiyama K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 456(1) Issue: 1 Pages: 1-6

    • DOI

      10.1016/j.bbrc.2014.10.128

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Osteogenic gene transcription is regulated via gap junction-mediated cell-cell communication.2015

    • Author(s)
      Mikami Y, Yamamoto K, Akiyama Y, Kobayashi M, Watanabe E, Watanabe N, Asano M, Shimizu N, Komiyama K.
    • Journal Title

      Stem Cells Dev.

      Volume: 24(2) Issue: 2 Pages: 214-27

    • DOI

      10.1089/scd.2014.0060

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Secretory leukocyte protease inhibitor による細胞移動能の制御機構に関する研究2017

    • Author(s)
      三上剛和, 福島敦史, 水谷祐輔, 早津学, 小宮山一雄, 牛木辰男
    • Organizer
      第122回日本解剖学会総会全国学術集会
    • Place of Presentation
      長崎大学坂本キャンパス(長崎県長崎市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion2016

    • Author(s)
      MIKAMI Y, TSUDA H, HAYAKAWA S.
    • Organizer
      第45回日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 骨芽細胞と隣接細胞間におけるギャップジャンクションを介した細胞間コミュニケーションの役割2016

    • Author(s)
      三上剛和
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 骨芽細胞による間葉系幹細胞の遺伝子発現制御機構の検討2015

    • Author(s)
      三上剛和,秋山祐子,山本清文,小林真之,渡辺恵理,渡辺信和,清水典佳,福島敦史
    • Organizer
      第1回日本骨免疫学会
    • Place of Presentation
      ホテルブリーズベイマリーナ(沖縄県宮古島市)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Osteoblasts regulate osteogenic gene transcription in mesenchymal stem cells via gap junctional cell-cell communication2015

    • Author(s)
      Mikami Y, Asano M, Komiyama K
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulation of osteogenic gnene transcription via gap junction-mediated cell-cell communication2014

    • Author(s)
      Mikami Y, Kitano T, Asano M, Komiyama K.
    • Organizer
      96th Annual meeting, scientific sessions and exhibition in conjugation with the Japanese society and Korean association of oral and maxillofacial surgeons
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2014-09-08 – 2014-09-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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