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Analysis of the space-time of the TPO/MPL signal control structure in the pulpal vascularization process

Research Project

Project/Area Number 26462800
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

Sunohara Masataka  日本歯科大学, 生命歯学部, 准教授 (70287770)

Co-Investigator(Kenkyū-buntansha) 森川 茂  国立感染症研究所, 獣医科学部, 部長 (00167686)
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsdental pulp / angiogenesis / vasculogenesis / tooth germ / in situ hybridization / 胎生期 / 歯胚 / 血管 / 歯髄
Outline of Final Research Achievements

In angiogenesis, differentiation and maturation of endothelial cells are considered to be stage-specifically and spatio-temporally controlled and also it has been reported that platelet hematopoiesis is involved in angiogenesis. By using in situ hybridization technique, stage specific expression of angiopoietin1, angiopoietin2, Tie-2, Vegf-R2 and c-mpl genes were observed in enamel organ, tooth papilla, tooth germ peripheral region in mouse embryos at days 14.5 post-coitum (p.c.) to p.c. 17.5. The stage-specific these genes have been detected and expression patterns of them suggested that there are some mechaniams contribute to neovascularization during tooth germ development.

Academic Significance and Societal Importance of the Research Achievements

本研究は歯髄幹細胞および幹細胞ニッチ相互間における時空間的(spatiotemporal)なWntシグナル制御機構の解明を基盤とし、TPO/MPLシグナル、NotchシグナルおよびAng1/Tie2シグナルとの相互作用の分子機構を明らかにすることにより、歯髄再生の臨床応用の可能性を詳細に検討することを目的とし、本研究により得られた知見は、歯髄における高次血管構築制御機構の解明、さらにはニッチシグナルを制御することによる歯髄再生医療の基盤の確立、他の生体各組織の高次構造および機能維持機構の解析への多大な貢献が期待できるものと考えられる。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (16 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Promoter motifs required for c-mpl gene expression induced by thrombopoietin in CMK cells.2017

    • Author(s)
      Sunohara M, Sato I, Morikawa S.
    • Journal Title

      Cellular and molecular biology [Cell. Mol. Biol. (Noisy-le-grand)]

      Volume: 63(11) Issue: 11 Pages: 119-123

    • DOI

      10.14715/cmb/2017.63.11.21

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Molecular control of vascular formation in developing mouse tooth germ2018

    • Author(s)
      Sunohara M, Morikawa S, Sato I
    • Organizer
      96th Annual Meeting and Exhibition of the International Association for Dental Research, London, England, PROGRAM BOOK, 169, 2018.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vasculogenesis and Angiogenesis during tooth germ development.2018

    • Author(s)
      M. Sunohara, S. Morikawa, I. Sato
    • Organizer
      International Meeting of the German Society for Cell Biology, Leipzig, Germany, DGZ-2019-MEETING GUIDE, Cell News 02/2018, P08-11, DGZ, 91, 2018.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス胎児血管発生過程における各種シグナル伝達物質の関与の検討2018

    • Author(s)
      春原正隆, 佐藤巌
    • Organizer
      第60回歯科基礎医学会学術大会ならびに総会プログラム集,359, 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dynamics in vascularization during tooth development2018

    • Author(s)
      Masataka Sunohara, Shigeru Morikawa Iwao Sato
    • Organizer
      第124回日本解剖学会総会・全国学術集会 講演プログラム・抄録集, 216, 2019.
    • Related Report
      2018 Annual Research Report
  • [Presentation] Signaling molecules in embryonic vascular development of tooth germ2018

    • Author(s)
      Masataka Sunohara, Shigeru Morikawa Iwao Sato
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Role of signaling molecules in developmental angiogenesis of mouse embryo2017

    • Author(s)
      Sunohara M, Morikawa S, Sato I
    • Organizer
      The 122th Annual Meeting of The Japanese Association of Anatomists
    • Place of Presentation
      長崎大学坂本キャンパス、長崎県長崎市
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Contribution of the signaling molecules during vascular development in tooth germ.2017

    • Author(s)
      Sunohara M, Morikawa S, Noguchi K, Sato I
    • Organizer
      Anatomische Gesellschaft, 112th Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 歯胚発生過程における血管形成制御因子の発現状況および局在の検討2017

    • Author(s)
      春原正隆,佐藤巌
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Crucial regulators of angiogenesis during tooth germ development2016

    • Author(s)
      Sunohara M, Morikawa S, Sato I
    • Organizer
      Anatomische Gesellschaft, 111th Annual Meeting
    • Place of Presentation
      Göttingen, Germany
    • Year and Date
      2016-09-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 歯胚血管新生過程における調節因子の同定2016

    • Author(s)
      春原正隆, 関伸一郎, 佐藤巌
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター、北海道札幌市
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Expression patterns of signaling molecules in vasculogenesis of mouse embryo2016

    • Author(s)
      Masataka Sunohara, Shigeru Morikawa, Iwao Sato
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Signaling molecules involved in initial formation of blood vessels during tooth development2016

    • Author(s)
      M. Sunohara, S. Morikawa, T. Yatsu, Y. Miwa, M. Yamazaki, I. Sato
    • Organizer
      45th Annual Meeting and Exhibition of the American Association for Dental Research
    • Place of Presentation
      Los Angeles, Calif.(USA)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular control of angiogenesis in tooth development2015

    • Author(s)
      Masataka Sunohara and Iwao Sato
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulatory mechanism in differentiation of mesenchymal cells during tooth development.2015

    • Author(s)
      Sunohara Masataka, Morikawa Shigeru, Sato Iwao
    • Organizer
      The 120th Annual Meeting of The Japanese Association of Anatomists
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular regulatory mechanism of angiogenesis during tooth morphogenesis.2014

    • Author(s)
      M.Sunohara, S.Morikawa, H.Murata, I.Sato
    • Organizer
      IADR/PER Congress & Exhibition 2014
    • Place of Presentation
      Dubrovnik, Croatia
    • Year and Date
      2014-10-10 – 2014-10-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯胚発生過程における血管新生制御機構の解析.2014

    • Author(s)
      春原正隆, 佐藤巌
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2020-03-30  

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