Mechanism of invasive diseases by superinfection with influenza virus and oral Streptococcus

• Project/Area Number: 26462806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kanazawa University
• Principal Investigator: OKAMOTO Shigefumi 金沢大学, 保健学系, 教授 (50311759)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 並行感染 / 口腔レンサ球菌 / インフルエンザウイルス / 感染症重症化 / 付着 / 付着侵入 / 混合感染 / Streptococcus sanguinis

Outline of Final Research Achievements

Superinfection with influenza A virus (IAV) and streptococci such as Streptococcus pneumoniae is known to cause severe infection. On the other hand, it is known that oral streptococci considered to be a low-pathogenic bacteria have high genomic homology with other pathogenic streptococci, thus, it is possible that superinfection with oral streptococci and IAV leads to invasive infections.
In this study, we showed that superinfection with IAV and one of the oral streptococci, Streptococcus sanguinis occurs severe pneumonia. It is suggested that the severe disease is caused that the enhanced gp96 expression in IAV-infected alveolar epithelial cells induces enhanced adhesion of S. sanguinis to the IAV-infected cells.

Research Products

• [Journal Article] Toll-like receptor 9 ligand D-type oligodeoxynucleotide D35 as a broad inhibitor for influenza A virus replication that is associated with suppression of neuraminidase activity (2016)
  • Author(s): Yamada H, Nagase S, Takahashi K, Sakoda Y, Kida H, Okamoto S.
  • Journal Title: Antiviral Research Volume: 129 Pages: 81-92
  • DOI: 10.1016/j.antiviral.2016.02.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Neuraminidase gene homology contributes to the protective activity of influenza vaccines prepared from the influenza virus library (2014)
  • Author(s): Haredy AM, Yamada H, Sakoda Y, Okamatsu M, Yamamoto N, Omasa T, Mori Y, Kida H, Okamoto S, Okuno Y, Yamanishi K
  • Journal Title: Journal of General Virology Volume: 95 Issue: 11 Pages: 2365-2371
  • DOI: 10.1099/vir.0.067488-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dextran sulfate-resistant A/Puerto Rico/8/34 influenza virus is associated with the emergence of specific mutations in the neuraminidase glycoprotein (2014)
  • Author(s): Yamada H, Nagao C, Haredy AM, Mori Y, Mizuguchi K, Yamanishi K, Okamoto S
  • Journal Title: Antiviral Research Volume: 111 Pages: 69-77
  • DOI: 10.1016/j.antiviral.2014.09.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 中鎖脂肪酸の抗菌効果の新規測定法の開発 (2017)
  • Author(s): 森裕美子, 松榮美希, 長瀬賢史, 小谷勇介, 湯淺善恵, 林和貴, 杉谷加代, 岡本成史
  • Organizer: 第90回日本細菌学会総会・学術集会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-19
• [Presentation] 新規皮膚細菌採取法の開発と同方法によるスワブ法と比較しての採取される皮膚細菌叢分布の相違 (2017)
  • Author(s): 長瀬賢史, 大貝和裕, 森裕美子, 向井加奈恵, 松榮美希, 須釜淳子, 岡本成史
  • Organizer: 第90回日本細菌学会総会・学術集会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-19
• [Presentation] ヴァージンココナッツオイル（VCO）とラウリン酸における抗菌効果の比較検討 (2016)
  • Author(s): 森裕美子, 松榮美希, 長瀬賢史, 小川真理子, 住友倫子, 中田匡宣, 川端重忠, 岡本成史
  • Organizer: 第53回日本細菌学会中部支部総会
  • Place of Presentation: 日本歯科大学新潟生命歯学部（新潟県新潟市）
  • Year and Date: 2016-10-28
• [Presentation] インフルエンザウイルスとレンサ球菌属との共感染による劇症型感染症発症に関する研究動向 (2016)
  • Author(s): 岡本成史
  • Organizer: 第８９回日本細菌学会総会学術集会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-23
  • Invited
• [Remarks] 金沢大学医薬保健学域保健学類 金沢大学大学院医薬保健学総合研究科保健学専攻
  • URL: http://mhs3.mp.kanazawa-u.ac.jp