Effect of CCN2 on the osteocyte function regulating the osteoclast formation, and its possibility as a novel drug of osteoporosis

• Project/Area Number: 26462810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Okayama University
• Principal Investigator: Nishida Takashi 岡山大学, 医歯薬学総合研究科, 准教授 (30322233)
• Co-Investigator(Kenkyū-buntansha): 滝川 正春 岡山大学, 医歯薬学総合研究科, 教授 (20112063) ; 久保田 聡 岡山大学, 医歯薬学総合研究科, 教授 (90221936) ; 服部 高子 岡山大学, 医歯薬学総合研究科, 助教 (00228488) ; 青山 絵理子 岡山大学, 医歯薬学総合研究科, 助教 (10432650)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: CCN2/CTGF / 骨細胞様細胞 / 破骨細胞形成 / タモキシフェン誘導性Ccn2欠損マウス / 3次元培養 / 老齢期のタモキシフェン誘導性CCN2欠損マウス / NFATc1 / カテプシンK / 骨細胞 / 初代骨細胞様細胞 / 薬剤誘導性Ccn2欠損マウス / マウス骨細胞株MLO-Y4

Outline of Final Research Achievements

It is well-known that osteocytes play a role in the regulation of bone remodeling. However, the effect of CCN2/CTGF on the bone remodeling in osteocytes is unknown. Therefore, the aim of this study is the investigation of osteoclastogenesis via osteocytes stimulated by CCN2. A mouse osteocytic cell line, MLO-Y4 embedded into collagen gel containing recombinant CCN2 protein, promoted the osteoclast differentiation of RAW264.7 cells inoculated on the collagen gel, and osteocyte-like cells isolated from Ccn2-deficient mice inhibited the differentiation of osteoclasts under the same condition. These findings suggest that osteoclastogenesis is modulated by osteocytes stimulated by CCN2.

Research Products

• [Int'l Joint Research] Department of Orthopedic surgery, UCLA/Department of Oral Biology, UMKC(米国)
• [Int'l Joint Research] Department of Orthopedic Surgery, UCLA,/Department of Oral Biology, UMKC(米国)
• [Journal Article] Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage. (2017)
  • Author(s): Janune D, Abd El Kader T, Aoyama E, Nishida T, Tabata Y, Kubota S, Takigawa M
  • Journal Title: J Bone Miner Metab. Volume: in press Issue: 6 Pages: 582-597
  • DOI: 10.1007/s00774-016-0793-4
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in the regeneration of articular cartilage: Mechanism underlying this stimulation. (2017)
  • Author(s): Nishida T, Kubota S, Aoyama E, Yamanaka N, Lyons KM, Takigawa M
  • Journal Title: Osteoarthritis Cartilage Volume: 25 Issue: 5 Pages: 759-769
  • DOI: 10.1016/j.joca.2016.10.003
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Catabolic effects of FGF-1 on chondrocytes and its possible role in osteoarthritis. (2017)
  • Author(s): El-Seoudi A, Abd El Kader T, Nishida T, Eguchi T, Aoyama E, Takigawa M, Kubota S
  • Journal Title: J Cell Commun Signal. Volume: in press Issue: 3 Pages: 255-263
  • DOI: 10.1007/s12079-017-0384-8
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Role of CCN2 in amino acid metabolism of chondrocytes (2016)
  • Author(s): Murase, Y,, Hattori, T., Aoyama, E., Nishida, T., Maeda-Uematsu, A., Kawaki, H., Lyons, K. M., Sasaki, A., Takigawa, M., Kubota, S
  • Journal Title: J. Cell Biochem Volume: 117 Issue: 4 Pages: 927-937
  • DOI: 10.1002/jcb.25377
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of multiple CCN family members in platelets that support regeneration of joint tissues. (2016)
  • Author(s): Hara, C., Kubota, S., Nishida, T., Hiasa, M., Hattori, T., Aoyama, E., Moriyama, Y., Kamioka, H., Takigawa, M.
  • Journal Title: Mod. Rheumatol. Volume: なし Issue: 6 Pages: 1-10
  • DOI: 10.3109/14397595.2016.1155255
  • NAID: 120006358661
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Production of recombinant CCN2 protein by mammalian cells. (2016)
  • Author(s): Nishida, T., Kubota, S., Takigawa, M.
  • Journal Title: Methods in Molecular Biology: CCN Proteins: Methods and Protocols Volume: 未定
• [Journal Article] Cell biological assays for measuring chondrogenic activities of CCN2 protein. (2016)
  • Author(s): Nishida, T., Kubota, S., Takigawa, M.
  • Journal Title: Methods in Molecular Biology: CCN Proteins: Methods and Protocols Volume: 未定
• [Journal Article] In vivo evaluation of cartilage regenerative effects of CCN2 protein. (2016)
  • Author(s): Nishida, T., Kubota, S., Takigawa, M.
  • Journal Title: Methods in Molecular Biology: CCN Proteins: Methods and Protocols Volume: 未定
• [Journal Article] CCN2 enhances RANKL-induced osteoclast differentiation via direct binding to RANK and OPG (2015)
  • Author(s): Aoyama E, Kubota S, Khattab HM, Nishida T, Takigawa M.
  • Journal Title: Bone Volume: 73 Pages: 242-248
  • DOI: 10.1016/j.bone.2014.12.058
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CCN family protein 2 (CCN2) promotes the early differentiation, but inhibits the terminal differentiation of skeletal myoblasts (2015)
  • Author(s): Nishida T, Kubota S, Aoyama E, Janune D, Lyons KM, Takigawa M.
  • Journal Title: J Biochem Volume: 157(2) Issue: 2 Pages: 91-100
  • DOI: 10.1093/jb/mvu056
  • NAID: 40020353020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] New functional aspects of CCN2 revealed by trans-omic approaches. (2015)
  • Author(s): Kubota, S., Maeda-Uematsu, A., Nishida, T., Takigawa, M
  • Journal Title: J. Oral Biosci. Volume: 57 Issue: 1 Pages: 37-43
  • DOI: 10.1016/j.job.2014.09.002
• [Journal Article] Direct interaction between CCN family protein 2 and fibroblast growth factor 1 (2014)
  • Author(s): 6)Abd El Kader T, Kubota S, Anno K, Tanaka S, Nishida T, Furumatsu T, Aoyama E, Kuboki T, Takigawa M.
  • Journal Title: J Cell Commun Signal Volume: 8(2) Issue: 2 Pages: 157-63
  • DOI: 10.1007/s12079-014-0232-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differential expression of vascular endothelial growth factor in high- and low- metastasis cell lines of salivary gland adenoid cystic carcinoma. (2014)
  • Author(s): Kondo, S., Mukudai, Y., Soga, D., Nishida, T., Takigawa, M., Shirota, T.
  • Journal Title: Anticancer Res. Volume: 34 Pages: 671-677
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CCN2 as a novel molecule supporting energy metabolism of chondrocytes. (2014)
  • Author(s): Maeda-Uematsu A, Kubota S, Kawaki H, Kawata K, Miyake Y, Hattori T, Nishida T, Moritani N, Lyons KM, Iida S, Takigawa M.
  • Journal Title: J Cell Biochem Volume: 115 Issue: 5 Pages: 854-865
  • DOI: 10.1002/jcb.24728
  • NAID: 120005468290
  • Peer Reviewed / Open Access
• [Journal Article] The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vitro (2014)
  • Author(s): Tarek Abd El Kader, et al.
  • Journal Title: Bone Volume: 59 Pages: 180-188
  • DOI: 10.1016/j.bone.2013.11.010
  • Peer Reviewed / Open Access
• [Presentation] 軟骨細胞分化に関わる長鎖ノンコーディングRNAの解析。 (2017)
  • Author(s): 石川崇典、村瀬友里香、西田 崇、服部高子、大野充昭、古松毅之、滝川正春、久保田聡：
  • Organizer: 第30回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-03
• [Presentation] 軟骨細胞のCCN2産生に対するセロトニン(5-HT)の制御機構の解明。 (2017)
  • Author(s): 堀 綾花、西田 崇、高柴正悟、久保田聡、滝川正春
  • Organizer: 第30回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-03
• [Presentation] Mechanism of the catabolic effects of Fibroblast Growth Factor (FGF-1) on chondrocytes and its possible role in Osteoarthritis. (2017)
  • Author(s): Elseoudi, A., Abd El Kader, T., Nishida T., Aoyama, E., Eguch, T., Takigawa, M., Kubota, S.
  • Organizer: 第30回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-03
• [Presentation] Effects of fibroblast growth factor 1 (FGF1) on chondrocytes and its possible role in osteoarthritis. (2016)
  • Author(s): Elseoudi, A., Abd El Kader, T., Nishida T., Aoyama, E., Eguch, T., Takigawa, M., Kubota, S.
  • Organizer: 第89回日本生化学会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] 軟骨組織におけるメラトニンの作用。 (2016)
  • Author(s): 服部高子、西田 崇、久保田聡
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-08-24
• [Presentation] タモキシフェン誘導性CCN2欠損マウス由来の骨細胞様細胞の破骨細胞形成能。 (2016)
  • Author(s): 西田 崇、久保田聡、滝川正春
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-07-24
• [Presentation] 骨細胞様細胞におけるCCN2の欠損は破骨細胞形成を抑制する。 (2016)
  • Author(s): 西田 崇、久保田聡、横井秀基、向山政志、滝川正春
  • Organizer: 第34回日本骨代謝学会学術集会 第3回アジア太平洋骨代謝学会議
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-20
• [Presentation] セロトニン(5-HT)による軟骨細胞におけるCCN2産生の作用機構の解明。 (2016)
  • Author(s): 堀 綾花、西田 崇、久保田聡、滝川正春
  • Organizer: 第34回日本骨代謝学会学術集会 第3回アジア太平洋骨代謝学会議
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-20
• [Presentation] 軟骨細胞形質に関わる長鎖非コードRNAの探索。 (2016)
  • Author(s): 石川崇典、村瀬友里香、西田 崇、服部高子、滝川正春、上岡 寛、久保田聡
  • Organizer: 第34回日本骨代謝学会学術集会 第3回アジア太平洋骨代謝学会議
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-20
• [Presentation] Investigation on long non-coding RNAs that are associated with chondrocytic phenotype. (2016)
  • Author(s): Ishikawa, T., Murase, Y., Nishida, T., Hattori, T., Takigawa, M., Kubota, S.
  • Organizer: RNA2016, June 28 - July 2,
  • Place of Presentation: 京都
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] 軟骨細胞形成を支える長鎖非コードRNA。 (2016)
  • Author(s): 石川崇典、久保田聡、村瀬有里香、西田 崇、服部高子、滝川正春
  • Organizer: 第29回日本軟骨代謝学会
  • Place of Presentation: 広島
  • Year and Date: 2016-02-19
• [Presentation] CCN2産生を誘導するセロトニンの軟骨細胞内でのシグナル伝達機構の解析。 (2016)
  • Author(s): 堀 綾花、西田 崇、高柴正悟、久保田聡、滝川正春
  • Organizer: 第29回日本軟骨代謝学会
  • Place of Presentation: 広島
  • Year and Date: 2016-02-19
• [Presentation] Mechanism of the catabolic effects of Fibroblast Growth Factor (FGF-1) on chondrocytes and its role in CCN2 regulation. (2016)
  • Author(s): Elseoudi, A., Abd El Kader, T., Nishida T., Aoyama, E., Eguch, T., Takigawa, M., Kubota, S
  • Organizer: 第8回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] 軟骨細胞におけるセロトニン（5－HT）のCCN2産生促進機構の解明。 (2016)
  • Author(s): 堀 綾花、西田 崇、高柴正悟、久保田聡、滝川正春
  • Organizer: 第8回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] 骨のリモデリング期のCCN2欠損は骨細胞由来の破骨細胞形成を抑制する。 (2016)
  • Author(s): 西田 崇、久保田聡、横井秀基、向山政志、滝川正春
  • Organizer: 第8回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] 軟骨細胞におけるCCN2産生に対するセロトニン(5-HT)の作用。 (2016)
  • Author(s): 堀 綾花、西田 崇、高柴正悟、久保田聡、滝川正春
  • Organizer: 第37回岡山歯学会
  • Place of Presentation: 岡山
• [Presentation] 骨細胞の作用を介した破骨細胞形成におけるCCN2の役割。 (2015)
  • Author(s): 西田 崇、久保田聡、服部高子、Bonewald, L. F.、 滝川正春
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Induction of CCN2 by low-intensity pulsed ultrasound (LIPUS) in cultured chondrocytes and its biological significance. (2015)
  • Author(s): Nishida, T., Kubota, S., Aoyama, E., Yamanaka, N., Lyons, K. M., Takigawa, M.
  • Organizer: Eight International Workshop on the CCN Family of Genes
  • Place of Presentation: Nice, France
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] Metabolic impacts of CCN2 in chondrocytes. (2015)
  • Author(s): Kubota, S., Murase, Y., Maeda-Uematsu, A., Hattori, T., Aoyama, E., Nishida, T., Kawaki, H., Lyons, K. M., Takigawa, M.
  • Organizer: Eight International Workshop on the CCN Family of Genes
  • Place of Presentation: Nice, France
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] Role of interaction between CCN2 and Rab14 in vesicle trafficking in chondrocytes novel intracellular function of CCN2. (2015)
  • Author(s): Hoshijima, M., Hattori, T., Aoyama, E., Nishida, T., Kubota, S., Kamioka, H., Takigawa, M.
  • Organizer: Eight International Workshop on the CCN Family of Genes
  • Place of Presentation: Nice, France
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] Regenerative effects of CCN2 ondependent modules and CCN3 on articular chondrocytes/cartilage. (2015)
  • Author(s): Takigawa, M., Abd El Kader, T., Janune, D., Aoyama, E., Nishida, T., Hattori, T., Hara, E. S., Ono, M., Tabata, Y., Kuboki, T., Kubota, S.
  • Organizer: Eight International Workshop on the CCN Family of Genes
  • Place of Presentation: Nice, France
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] CCN2は骨細胞を介して破骨細胞形成を制御する。 (2015)
  • Author(s): 西田 崇、久保田聡、服部高子、滝川正春
  • Organizer: 第57回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割 ～軟骨分化促進因子CCN2の新たな細胞内機能～。 (2015)
  • Author(s): 星島光博、服部高子、青山絵理子、西田 崇、久保田聡、上岡 寛、滝川正春
  • Organizer: 第57回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] Introducing CCN2 independent modules as a regenerative therapy for osteoarthritis and futher selecting the most suitable among them. (2015)
  • Author(s): Abd El-Kader, T., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Hara, E. S., Ono M., Tabata, Y., Kuboki, T., Kubota, S., Takigawa, M.
  • Organizer: 第7回日本CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-08-29
• [Presentation] CCN2の新たな細胞内機能 : CCN2 とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割。 (2015)
  • Author(s): 星島光博、服部高子、青山絵理子、西田 崇、久保田聡、上岡 寛、滝川正春
  • Organizer: 第7回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-08-29
• [Presentation] CCN2による軟骨細胞アミノ酸代謝制御機構の解明。 (2015)
  • Author(s): 村瀬有里香、服部高子、青山絵理子、西田 崇、前田 彩、川木晴美、Lyons, K. M., 佐々木朗、滝川正春、久保田聡
  • Organizer: 第7回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-08-29
• [Presentation] 関節軟骨におけるCCN3の新機能。 (2015)
  • Author(s): ジャヌネ ダニーロ、Abd El-Kader, T., 青山絵理子、西田 崇、久保田聡、滝川正春
  • Organizer: 第7回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-08-29
• [Presentation] CCN2は骨細胞に作用し、破骨細胞を正に制御する。 (2015)
  • Author(s): 西田 崇、久保田聡、服部高子、Bonewald, L. F., 滝川正春
  • Organizer: 第7回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-08-29
• [Presentation] 破骨細胞形成を制御する骨細胞に与えるCCN2の作用。 (2015)
  • Author(s): 西田 崇、久保田聡、滝川正春
  • Organizer: 第33回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-23
• [Presentation] 関節軟骨におけるCCN3の新機能。 (2015)
  • Author(s): ジャヌネ ダニーロ、Abd El-Kader, T., 青山絵理子、西田 崇、Khattab, H., 久保田聡、滝川正春
  • Organizer: 第33回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-23
• [Presentation] CCN2による軟骨細胞のアミノ酸代謝制御。 (2015)
  • Author(s): 村瀬有里香、服部高子、青山絵理子、西田 崇、前田 彩、川木晴美、佐々木朗、滝川正春、久保田聡
  • Organizer: 第33回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-23
• [Presentation] 軟骨細胞アミノ酸代謝とCCN2。 (2015)
  • Author(s): 村瀬有里香、服部高子、青山絵理子、西田 崇、前田 彩、川木晴美、Lyons, K. M.、佐々木朗、滝川正春、久保田聡
  • Organizer: 第6回骨バイオサイエンス研究会
  • Place of Presentation: 岡山
  • Year and Date: 2015-07-04
• [Presentation] E6-AP/UBE3A Protein, a Ubiquitin Ligase toward SOX9 Protein is essential to endochondral ossification. (2015)
  • Author(s): Hattori, T., Kishino, T., Stephen, S.,Eberspaecher, H., Maki, S., Takigawa, M., Nishida, T., Kubota, S., de Crombrugghe, B., Yasuda, H.
  • Organizer: Gordon Research Conference, Cartilage Biology &Pathology
  • Place of Presentation: Galveston, TX, USA
  • Year and Date: 2015-03-22 – 2015-03-27
• [Presentation] CCN2とRab14の相互作用が軟骨細胞の小胞輸送に及ぼす役割。 (2015)
  • Author(s): 星島光博、服部高子、青山絵理子、西田 崇、上岡 寛、滝川正春
  • Organizer: 第28回日本軟骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-06 – 2015-03-07
• [Presentation] 軟骨細胞分化に与えるセロトニンの作用。 (2015)
  • Author(s): 西田 崇、久保田聡、滝川正春
  • Organizer: 第28回日本軟骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-06 – 2015-03-07
• [Presentation] SOX9のユビキチンリガーゼE6-AP/UBE3Aは正常な骨格形成に必須である。 (2014)
  • Author(s): 服部高子、木住野達也、Shelley Stephen、Heidi Eberspaecher、巻さゆみ、滝川正春、西田 崇、久保田聡、Benoit de Crombrugghe、安田秀世
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Assessment of the regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarturiritis models in vivo. (2014)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Hara, E. S., Ono, M., Tabata, Y., Kuboki, T., Takigawa, M.
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] CCN2は筋芽細胞の初期分化を促進するが、後期分化を抑制する。 (2014)
  • Author(s): 西田 崇、久保田聡、青山絵理子、ダニーロ ジャヌネ、カレン ライアン、滝川正春
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] トランスオーミックアプローチによるCCN2の代謝支持機能の解明。 (2014)
  • Author(s): 久保田聡、村瀬友里香、植松-前田 彩、服部高子、西田 崇、滝川正春
  • Organizer: 第6回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2014-08-30
• [Presentation] 高分化型および低分化型ヒト口腔癌細胞株におけるCCNファミリータンパク質の発現。 (2014)
  • Author(s): 高嶋荘一朗、西田 崇、久保田聡、滝川正春
  • Organizer: 第6回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2014-08-30
• [Presentation] CCN2に依存した低出力超音波(LIPUS)の軟骨細胞への作用メカニズムの解明。 (2014)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、Lyons, K. M., 滝川正春
  • Organizer: 第6回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2014-08-30
• [Presentation] RANKデコイレセプターOPGとCCN2の相互作用。 (2014)
  • Author(s): 青山絵理子、西田 崇、久保田聡、滝川正春
  • Organizer: 第6回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2014-08-30
• [Presentation] The regenerative effect of CCN2 independent modules on chondrocytes in vitro and osteoarturiritis models in vivo. (2014)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E.,Janune, D., Hara, E. S., Ono, M., Tabata, Y., Kuboki, T., Takigawa, M.
  • Organizer: 第6回CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2014-08-30
• [Presentation] 軟骨分化促進因子CCN2の新たな細胞内機能：CCN2とRab14GTPaseの相互作用が軟骨細胞の小胞輸送に及ぼす役割。 (2014)
  • Author(s): 星島光博、服部高子、青山絵理子、西田 崇、上岡 寛、滝川正春
  • Organizer: 第32回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] 培養軟骨細胞における低出力性超音波(LIPUS)によるCCN2産生亢進機構の解明 (2014)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、Lyons, K. M., 滝川正春
  • Organizer: 第32回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] LIPUS promotes CCN2-mediated cartilage matrix gene expression via MAPK pathways. (2014)
  • Author(s): Nishida, T., Kubota, S., Aoyama, E., Yamanaka, N., Takigawa, M.
  • Organizer: 92nd IADR
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-06-25 – 2014-06-28
• [Presentation] CCN2 induces osteoclastogenesis by regulating RANK/RANKL/OPG system. (2014)
  • Author(s): Aoyama E, Kubota S, Nishida T, Takigawa M
  • Organizer: ECTS Congress 2014
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2014-05-17 – 2014-05-20
• [Remarks] 岡山大学歯学部口腔生化学分野
• [Remarks] 岡山大学歯学部口腔生化学分野
• [Remarks] 岡山大学歯学部口腔生化学分野