functional analysis of TMEM16E through its subsellular localozation.

• Project/Area Number: 26462813
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Tobiume Kei 広島大学, 医歯薬保健学研究院(歯), 准教授 (40350037)
• Co-Investigator(Kenkyū-buntansha): 水田 邦子 広島大学, 医歯薬保健学研究院(歯), 助教 (40432679)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: TMEM16E / Ano5 / LGMD2L / GDD1 / Anoctamin5 / GDD / Anoctamin 5 / Gdd1 / LGMD2 / TMEM16 / caveolin / anoctamin / dysferlin / membrane traffic

Outline of Final Research Achievements

We developed a validated immunocytochemistry protocol for rabbit polyclonal TMEM16E
antibody in this study. Essentially, a special cell fixation reagent with a suitable detergent in a reaction buffer to erase non-specific reactions. By this sophisticated protocol, We identified TMEM16E/Caveolin3-carrying vesicle like structures in cultured myotube, which translocated just beneath plasma membrane in dysferlin-defect myotubes.

Research Products

• [Journal Article] CD44(high) /ALDH1(high) head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β-dependent cancer stem cell properties. (2016)
  • Author(s): Seino S, Shigeishi H, Hashikata M, Higashikawa K, Tobiume K, Uetsuki R, Ishida Y, Sasaki K, Naruse T, Rahman MZ, Ono S, Simasue H, Ohta K, Sugiyama M, Takechi M.
  • Journal Title: J Oral Pathol Med Volume: 45 Issue: 3 Pages: 180-188
  • DOI: 10.1111/jop.12348
  • Peer Reviewed / Open Access
• [Journal Article] p53 regulates cytoskeleton remodeling to suppress tumor progression. (2015)
  • Author(s): Araki K, Ebata T, Guo AK, Tobiume K, Wolf SJ, Kawauchi K.
  • Journal Title: Cell Mol Life Sci. Volume: 72(21) Issue: 21 Pages: 4077-94
  • DOI: 10.1007/s00018-015-1989-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression and function of RIG-I in oral keratinocytes and fibroblasts (2014)
  • Author(s): Ohta K, Fukui A
  • Journal Title: Cell Phsiol Biochem Volume: 34 Issue: 5 Pages: 1556-1565
  • DOI: 10.1159/000366359
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Toll-like receptor (TLR) expression and TLR‑mediated interleukin-8 production by human submandibular gland epithelial cells. (2014)
  • Author(s): Ohta K, Ishida Y, Fukui A, Mizuta K, Nishi H, Takechi M, Kamata N.
  • Journal Title: Molecular medicine reports. Volume: 10（5） Issue: 5 Pages: 2377-2382
  • DOI: 10.3892/mmr.2014.2507
  • Peer Reviewed / Open Access
• [Presentation] GDD1/ANO5/TMEM16Eの細胞内分布局在 (2016)
  • Author(s): 飛梅圭、久保薗和美、水田邦子
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜