| Project/Area Number |
26462813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Tobiume Kei 広島大学, 医歯薬保健学研究院(歯), 准教授 (40350037)
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| Co-Investigator(Kenkyū-buntansha) |
水田 邦子 広島大学, 医歯薬保健学研究院(歯), 助教 (40432679)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | TMEM16E / Ano5 / LGMD2L / GDD1 / Anoctamin5 / GDD / Anoctamin 5 / Gdd1 / LGMD2 / TMEM16 / caveolin / anoctamin / dysferlin / membrane traffic |
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| Outline of Final Research Achievements |
We developed a validated immunocytochemistry protocol for rabbit polyclonal TMEM16E
antibody in this study. Essentially, a special cell fixation reagent with a suitable detergent in a reaction buffer to erase non-specific reactions. By this sophisticated protocol, We identified TMEM16E/Caveolin3-carrying vesicle like structures in cultured myotube, which translocated just beneath plasma membrane in dysferlin-defect myotubes.
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