| Project/Area Number |
26462821
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
Nezu Akihiro 北海道医療大学, 歯学部, 准教授 (00305913)
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| Co-Investigator(Kenkyū-buntansha) |
石井 久淑 北海道医療大学, 歯学部, 教授 (00275489)
森田 貴雄 北海道医療大学, 歯学部, 講師 (20326549)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 唾液腺 / Ca2+応答 / 唾液分泌 / Ca2+イメージング / 血流動態 / 細胞内カルシウム |
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| Outline of Final Research Achievements |
We examined sialogogue-induced changes in Ca2+ response and blood flow (BF) during salivary secretion in rat submandibular gland (SMG) using an intravital Ca2+ imaging system, a fiber-optic pressure sensor and a laser speckle imaging flowmeter. Using the macro and micro Ca2+ imaging in rat SMG, we found that infusion with acetylcholine (ACh) induced tissue-wide synchronization of Ca2+ oscillations in vivo. Simultaneous monitoring of Ca2+ responses and BF in SMG demonstrated that the ACh-induced changes in BF were also synchronized with Ca2+ oscillations. We revealed that the vasoconstriction via angiotensin II and thromboxane A2 receptors play important role for a generation of the ACh-induced Ca2+ oscillations in SMG in vivo. Real-time monitoring of Ca2+ and salivary secretion showed a clear time lag between the onset of intracellular Ca2+ concentration ([Ca2+]i) and that of salivary secretion. Based on this result, we estimated required [Ca2+]i for salivary secretion in vivo.
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