Investigation of sensory integration in the insular cortex -multiple in vivo patch clamp analysis-

• Project/Area Number: 26462824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Meikai University
• Principal Investigator: Kazunori Adachi 明海大学, 歯学部, 准教授 (20349963)
• Co-Investigator(Kenkyū-buntansha): 小林 真之 日本大学, 歯学部, 教授 (00300830)
• Research Collaborator: AOKI Ryuhei ; NAGAO Takahide ; ODAI Reina ; SAITO Yoshihiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 島皮質 / 侵害受容 / 錐体細胞 / patch clamp / ラット / in vivo / whole-cell patch clamp

Outline of Final Research Achievements

The spontaneous membrane potential oscillation of cortical pyramidal neuron is characterized by up state, a short depolarization period, and down state that included following after hyperpolarization (AHP) and resting membrane potential. Up state of agranular insular (AI) cortical pyramidal neurons was less exhibited compared with those of somatosensory cortical neurons. GABAA agonist increased and prolonged AHP. Electrical tongue stimulation induced EPSPs in AI neurons located 1 mm rostral from middle central artery with high incidence. In more rostral region, same stimulation induced IPSPs with low incidence.

Research Products

• [Journal Article] Investigating complex basal ganglia circuitry in the regulation of motor behaviour, with particular focus on orofacial movement (2014)
  • Author(s): Adachi K et al.
  • Journal Title: Behav Pharmacol Volume: 26 Issue: 1 and 2 - Special Issue Pages: 18-32
  • DOI: 10.1097/fbp.0000000000000118
  • Peer Reviewed
• [Journal Article] 立効散鎮痛効果の仮性疼痛モデルによる検討 (2014)
  • Author(s): 安達 一典ら
  • Journal Title: 歯科薬物療法 Volume: 33 Pages: 1-9
  • NAID: 130004688266
  • Peer Reviewed
• [Presentation] Membrane potential oscillation in the agranular insular pyramidal neuron is mediated by GABAergic input (2016)
  • Author(s): Kazunori Adachi, Hiroshi Sakagami and Masayuki Kobayashi
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Duego
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] "The responses of the agranular insular pyramidal neuron to oral stimulation" (2016)
  • Author(s): Kazunori Adachi, Masayuki Kobayashi, Hiroshi Sakagami and Noriaki Koshikawa
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-09
• [Presentation] Pyramidal neurons in the agranular insular cortex receives sensory inputs from the tongue: an in vivo whole-cell patch-clamp study (2015)
  • Author(s): Kazunori Adachi, Masayuki Kobayashi, Hiroshi Sakagami and Noriaki Koshikawa
  • Organizer: Neouroscience 2015
  • Place of Presentation: シカゴ
  • Year and Date: 2015-10-17
• [Presentation] 島皮質侵害受容に関与する神経機構のin vivoならびにin vitroアプローチを併用した解析 (2014)
  • Author(s): 安達 一典
  • Organizer: 明海歯科医学会第23回学術大会
  • Place of Presentation: 明海大学歯学部
  • Year and Date: 2014-06-05