• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Bioluminescence imaging for osteogenic protein secretion

Research Project

Project/Area Number 26462828
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

Suzuki Takahiro  愛知学院大学, 歯学部, 准教授 (70298545)

Co-Investigator(Kenkyū-buntansha) 水野 光政  愛知学院大学, 歯学部, 助教 (20609812)
Co-Investigator(Renkei-kenkyūsha) INOUYE Satoshi  JNC(株), 横浜研究所, 企画研究員 (40426622)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsイメージング / 生物発光 / 開口分泌 / 遺伝子発現 / 3D培養 / 細胞接着 / タンパク質分泌 / ルシフェラーゼ / 骨芽細胞 / 骨形成因子 / エキソサイトーシス / 分泌 / 骨形成
Outline of Final Research Achievements

A simple method for preparing codon-optimized gene by choosing only codons preferentially used in human genes was named “preferred human codon-optimization method”. We demonstrated that the preferred human codon-optimized luciferase genes were efficiently expressed in several mammalian cells including osteoblastic cells. Also, we expressed a mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ), a preferred codon-optimized gene, and visualized it by bioluminescence imaging. Furthermore, we demonstrated quantitative visualization of protein secretion from 3D-cultured cells by the method of video-rate bioluminescence imaging using Gaussia luciferase as a reporter. Finally, we succeeded in visualizing polarized protein secretion from osteoblastic cells.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 5 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: identification of secretion sites in the intercellular contact regions2017

    • Author(s)
      Yokawa, S., Suzuki, T., Inouye, S., Inoh, Y., Suzuki, R., Kanamori, T., Furuno, T., Hirashima, N.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 485 Issue: 4 Pages: 725-730

    • DOI

      10.1016/j.bbrc.2017.02.114

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Quantitative visualization of synchronized insulin secretion from 3D-cultured cells.2017

    • Author(s)
      Suzuki T, Kanamori T, Inouye S.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 486 Issue: 4 Pages: 886-892

    • DOI

      10.1016/j.bbrc.2017.03.105

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells.2016

    • Author(s)
      Inouye S, Suzuki T
    • Journal Title

      Protein Expr. Purif.

      Volume: 128 Pages: 93-100

    • DOI

      10.1016/j.pep.2016.08.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Codon optimization of genes for efficient protein expression in mammalian cells by selection of only preferred human codons.2015

    • Author(s)
      Inouye S, Sahara-Miura Y, Sato J, Suzuki T.
    • Journal Title

      Protein Expr Purif.

      Volume: 109 Pages: 47-54

    • DOI

      10.1016/j.pep.2015.02.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells.2014

    • Author(s)
      Inouye S, Sato J-I, Sahara-Miura Y, Hosoya T, Suzuki T
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 450 Issue: 4 Pages: 1313-1319

    • DOI

      10.1016/j.bbrc.2014.06.140

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 細胞接着を介した膵島α細胞のグルカゴン分泌調節機構の解明2016

    • Author(s)
      横川慧, 鈴木崇弘, 井上敏, 伊納義和, 鈴木亮, 古野忠秀, 平嶋尚英
    • Organizer
      第38回生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      名古屋市立大学薬学部(愛知県名古屋市)
    • Year and Date
      2016-11-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生物発光イメージング法を用いた膵島α細胞からのグルカゴン分泌の可視化解析2016

    • Author(s)
      横川慧, 鈴木崇弘, 井上敏, 伊納義和, 鈴木亮, 古野忠秀, 平嶋尚英
    • Organizer
      第25回日本バイオイメージング学会学術集会
    • Place of Presentation
      名古屋市立大学薬学部(愛知県名古屋市)
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膵β細胞塊における同調性インスリン分泌の可視化2016

    • Author(s)
      鈴木崇弘, 井上敏
    • Organizer
      第15回生命科学研究会
    • Place of Presentation
      メルパルク東京(東京都港区)
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 哺乳類細胞における効率的なルシフェラーゼ遺伝子発現のためのコドン最適化と生物発光イメージング: 歯学研究への応用2015

    • Author(s)
      鈴木崇弘, 江頭美喜, 河合逹志, 金森孝雄, 井上敏
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ( 新潟県新潟市 )
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi