Bioluminescence imaging for osteogenic protein secretion
| Project/Area Number |
26462828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
水野 光政 愛知学院大学, 歯学部, 助教 (20609812)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUYE Satoshi JNC(株), 横浜研究所, 企画研究員 (40426622)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | イメージング / 生物発光 / 開口分泌 / 遺伝子発現 / 3D培養 / 細胞接着 / タンパク質分泌 / ルシフェラーゼ / 骨芽細胞 / 骨形成因子 / エキソサイトーシス / 分泌 / 骨形成 |
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| Outline of Final Research Achievements |
A simple method for preparing codon-optimized gene by choosing only codons preferentially used in human genes was named “preferred human codon-optimization method”. We demonstrated that the preferred human codon-optimized luciferase genes were efficiently expressed in several mammalian cells including osteoblastic cells. Also, we expressed a mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ), a preferred codon-optimized gene, and visualized it by bioluminescence imaging. Furthermore, we demonstrated quantitative visualization of protein secretion from 3D-cultured cells by the method of video-rate bioluminescence imaging using Gaussia luciferase as a reporter. Finally, we succeeded in visualizing polarized protein secretion from osteoblastic cells.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: identification of secretion sites in the intercellular contact regions2017
Author(s)
Yokawa, S., Suzuki, T., Inouye, S., Inoh, Y., Suzuki, R., Kanamori, T., Furuno, T., Hirashima, N.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 485
Issue: 4
Pages: 725-730
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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