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Establishment of physiological time measurement using saliva

Research Project

Project/Area Number 26462830
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Onishi Yoshiaki  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 副研究部門長 (60233219)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords唾液 / 転写 / クロマチン / 生物時計 / 生体時刻 / 概日リズム
Outline of Final Research Achievements

Establishment of physiological time measurement using saliva is required for the advanced dental care based on biological rhythms. Then, we have found ARRB1 as a candidate of the marker for physiological time measurement in saliva from the results of the gene expression profile. ARRB1 protein showed the circadian profile even using various saliva samples, suggesting a usefulness of this marker protein. We also found that the buffer containing 4 M Urea is adequate for saliva storage to prevent proteolysis and that the rhythm in saliva should be corrected using Actin as an internal control.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014

All Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Presentation] Shikonin shortens the circadian period possible role of topoisomerases, Yin-Yang action.2016

    • Author(s)
      大西 芳秋
    • Organizer
      7th World Gene Congress Convention-2016
    • Place of Presentation
      Gheraton Shanghai Waigaoqiao Hotel
    • Year and Date
      2016-11-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Herbs for Circadian Rhythm2016

    • Author(s)
      大西 芳秋
    • Organizer
      34th Annual meeting of Indian Academy of Neurosciences
    • Place of Presentation
      National Brain Research Center, Manesar, India
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Establishment of the peripheral clock system during eutherian embriohenesis: Critical role of Casein Kinase and Jmjd3.2015

    • Author(s)
      西本正純、奥田晶彦、大西芳秋
    • Organizer
      BMB 2015
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] シコニンによる培養細胞のコレステロール吸収の促進2015

    • Author(s)
      小川昌克、河野泰広、山崎幸苗、宮崎歴、大西芳秋
    • Organizer
      BMB 2015
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Inductions of Per2 and CKI epsilon expression are critical for establishment of circadian oscillation during eutherian embryogenesis.2014

    • Author(s)
      西本正純、奥田晶彦、大西芳秋
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Shikonin shortens the circadian period: Possible involvement of Top2 inhibition.2014

    • Author(s)
      小川昌克、河野泰広、山崎幸苗、大西芳秋
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館、京都
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 時計遺伝子プロモーター領域のDNAメチル化と概日リズム調節2014

    • Author(s)
      佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
    • Organizer
      第63回日本口腔衛生学会総会
    • Place of Presentation
      熊本市民会館崇城大学ホール/熊本市国際交流会館、熊本
    • Year and Date
      2014-05-31
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 概日リズム調整剤2015

    • Inventor(s)
      大石勝隆、宮崎歴、大西芳秋、伊藤奈々子、冨田辰之介、福留真一、等
    • Industrial Property Rights Holder
      大石勝隆、宮崎歴、大西芳秋、伊藤奈々子、冨田辰之介、福留真一、等
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-04-01
    • Related Report
      2015 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 生体リズムの制御剤2014

    • Inventor(s)
      山崎幸苗、河野泰広、大西芳秋、大石勝隆
    • Industrial Property Rights Holder
      山崎幸苗、河野泰広、大西芳秋、大石勝隆
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-09-24
    • Acquisition Date
      2015-07-17
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 生体リズムの制御剤2014

    • Inventor(s)
      山崎幸苗、河野泰広、大西芳秋、大石勝隆
    • Industrial Property Rights Holder
      山崎幸苗、河野泰広、大西芳秋、大石勝隆
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-194023
    • Filing Date
      2014-09-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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