Establishment of physiological time measurement using saliva
| Project/Area Number |
26462830
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Onishi Yoshiaki 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 副研究部門長 (60233219)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 唾液 / 転写 / クロマチン / 生物時計 / 生体時刻 / 概日リズム |
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| Outline of Final Research Achievements |
Establishment of physiological time measurement using saliva is required for the advanced dental care based on biological rhythms. Then, we have found ARRB1 as a candidate of the marker for physiological time measurement in saliva from the results of the gene expression profile. ARRB1 protein showed the circadian profile even using various saliva samples, suggesting a usefulness of this marker protein. We also found that the buffer containing 4 M Urea is adequate for saliva storage to prevent proteolysis and that the rhythm in saliva should be corrected using Actin as an internal control.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] Herbs for Circadian Rhythm2016
Author(s)
大西 芳秋
Organizer
34th Annual meeting of Indian Academy of Neurosciences
Place of Presentation
National Brain Research Center, Manesar, India
Year and Date
2016-10-19
Related Report
Int'l Joint Research / Invited
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