Roles of interleukin-33 in gingival Inflammation

• Project/Area Number: 26462834
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Ohno Tatsukuni 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (80435635)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: IL-33 / 歯周炎 / マウスモデル / 歯周病 / 歯肉炎モデル / 歯周炎モデル / LPS / P. gingivalis / 歯肉炎

Outline of Final Research Achievements

Interleukin-33 (IL-33), which is a new member of the IL-1 family of cytokines, has been involved in Th2-mediated immune responses including host defense against nematode and allergic diseases. Unlike regular cytokines, IL-33 is released from necrotic damaged cells during tissue injury and acts as an alarmin. Thus, IL-33 may contribute to chronic inflammatory tissue diseases. In this study, we investigate the roles of IL-33 in the gingival inflammation. Wild-type and IL-33 knockout (KO) mice received Porphyromonas gingivalis (P.g)-injection into the molar gingiva and gingival inflammatory responses were evaluated. LPS-injection into gingiva enhanced IL-1b, TNF-a, and IL-33 mRNA expression in wild type mice, but not in IL-33 KO mice. Our results suggest dual roles of IL-33; protective role in early gingival inflammatory responses.

Research Products

• [Journal Article] Immune checkpoint molecule, VISTA regulates T cell-mediated skin inflammatory responses (2017)
  • Author(s): Ohno T, Kondo Y, Zhang C, Kang S, Azuma M
  • Journal Title: J Invest Dermatol Volume: 印刷中 Issue: 6 Pages: 1384-1386
  • DOI: 10.1016/j.jid.2016.10.049
  • Peer Reviewed
• [Journal Article] An Interleukin-33-Mast Cell-Interleukin-2 Axis Suppresses Papain-Induced Allergic Inflammation by Promoting Regulatory T Cell Numbers. (2015)
  • Author(s): Morita H, Arae K, Unno H, Miyauchi K, Toyama S, Nambu A, Oboki K, Ohno T, Motomura K, Matsuda A, Yamaguchi S, Narushima S, Kajiwara N, Iikura M, Suto H, McKenzie AN, Takahashi T, Karasuyama H, Okumura K, Azuma M, Moro K, Akdis CA, Galli SJ, Koyasu S, Kubo M, Sudo K, Saito H, Matsumoto K, Nakae S.
  • Journal Title: Immunity Volume: 43 Issue: 1 Pages: 175-86
  • DOI: 10.1016/j.immuni.2015.06.021
  • NAID: 40020962854
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Endogenous and exogenous IL-33 promotes antitumor immunity (2015)
  • Author(s): Nishii Naoto, BHINGARE Arundhati, OHNO Tatsukuni, KONDO Yuta, AZUMA Miyuki
  • Organizer: 第44回日本免疫学会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Involvement of endogenous or exogenous IL-33 in antitumor immunity (2015)
  • Author(s): 西井 直人，Arundhati Bhingare，近藤 雄太，大野 建州，東みゆき
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市）
  • Year and Date: 2015-10-08