Development of combination theray with hyperthermia and Interleukin-1 inhibitor.

• Project/Area Number: 26462847
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Yokohama City University
• Principal Investigator: SENRI Oguri 横浜市立大学, 医学部, 助教 (30400394)
• Co-Investigator(Kenkyū-buntansha): 中島 英行 横浜市立大学, 医学研究科, 客員研究員 (30437032) ; 來生 知 横浜市立大学, 医学部, 准教授 (30545059) ; 光藤 健司 横浜市立大学, 医学部, 准教授 (70303641)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 進行口腔癌 / IL-1R / 温熱療法 / 併用療法 / IL-1 / 口腔癌 / 分子標的薬 / IL-1阻害薬

Outline of Final Research Achievements

To develop new therapies that are highly efficient and safe for advanced oral cancer, we have started studing of hyperthermia treatment combined with targeted therapy using Interleukin-1（IL-1）inhibitor. We have expected to the synergestic efficacy for modulating the expression of targeting molecule by hyperthermia.
In our results, the expression level of IL-1R was increased after hyperthermia in OSC-19 cells, which is one of the oral squamous cell carcinoma cell line, by western blotting. Moreover, cell motility assay showed that the group of which treated with combination therapy with both IL-1Ra (IL-1 inhibitor) and hyperthermia in OSC-19 cells, significantly reduced the cell motility compared to other groups.

Research Products

• [Presentation] 局所進行下顎歯肉癌に対する逆行性超選択的動注化学放射線療法 (2017)
  • Author(s): 小栗千里，光藤健司，大橋伸英，飯坂友宏，大久保牧子，馬場隼一，大屋貴志，岩井俊憲，岡本喜之，小泉敏之，廣田 誠，來生 知，藤内 祝
  • Organizer: 第35回日本口腔腫瘍学会総会・学術大会
• [Presentation] Recent advances in molecular-targeted therapy for oral cancer. (2017)
  • Author(s): Mitomu Kioi
  • Organizer: International Conference on Oral and Maxillofacial Surgery
  • Int'l Joint Research
• [Presentation] ヒト口腔扁平上皮癌細胞に対するmild-hyperthermiaとcetuximab併用効果の検討． (2016)
  • Author(s): 飯坂友宏，中島英行，來生 知，大久保牧子，杉浦 圭，小栗千里，小泉敏之，光藤健司，藤内 祝
  • Organizer: 日本ハイパーサーミア学会第34回大会
  • Place of Presentation: つくば国際会議場(茨城県つくば市）
  • Year and Date: 2016-09-02
• [Presentation] 局所進行下顎歯肉癌T4症例の検討. (2016)
  • Author(s): 小栗千里, 光藤健司, 小泉敏之, 大橋伸英, 柴崎麻衣子, 飯田昌樹, 岩井俊憲, 中島英行, 廣田 誠, 來生 知，藤内 祝
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: ソニックシティー（埼玉県大宮市）
  • Year and Date: 2016-06-09
• [Presentation] Combination therapy with Hyperthermia enhances the therapeutic efficacy of IL-13 cytotoxin in human oral squamous cell carcinoma. (2016)
  • Author(s): H. Nakashima, T. Iisaka, M. Kioi, A. Miyajima, M. Okubo, K. Sugiura, S. Itaru, S. Murata, K. Mitsudo and I. Tohnai
  • Organizer: The 12th International congress of hyperthermic oncology
  • Place of Presentation: InterContinental Hotel （ニューオリンズ、アメリカ）
  • Year and Date: 2016-04-11
• [Presentation] 口腔癌における温熱療法と分子標的薬の併用療法の開発 (2015)
  • Author(s): 中島英行、大久保牧子、宮島章嘉、飯坂友宏、杉浦 圭、飯田昌樹、佐藤 格、小泉俊之、來生 知、光藤健司，藤内 祝
  • Organizer: 日本ハイパーサーミア学会第32回大会 シンポジウム
  • Place of Presentation: KKRホテル大阪（大阪府大阪市）
  • Year and Date: 2015-09-04
• [Presentation] Chemoradiotherapy using retrograde superselective intra-arterial infusion for locally advanced tongue cancer: Effect for parapharyngeal space. (2015)
  • Author(s): Oguri S, Mitsudo K, Koizumi T, Ida M, Iwai T, Nakasima H, Hirota M, Kioi M, Iwai T
  • Organizer: The Joint Meeting of 4th Congress of Asian Society of Head and Neck Oncology and 39th Annual Meeting of Japan Society for Head and Neck Cancer
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-06-03
  • Int'l Joint Research