Influence of periodontal disease-causing bacteria infection on peritoneal dialysis

• Project/Area Number: 26462849
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nagoya City University
• Principal Investigator: IMAI Masaki 名古屋市立大学, 大学院医学研究科, 講師 (30440936)
• Co-Investigator(Kenkyū-buntansha): 太田 里永子 愛知県がんセンター(研究所), その他部局等, 研究員 (30452460)
• Research Collaborator: MIZUNO Masashi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 腹膜透析 / 歯周病

Outline of Final Research Achievements

To assess influence of periodontal disease-causing bacteria infection on peritoneal dialysis (PD), we investigated the relationship between periodontal pathogenic bacterial infection an the tissue damage associated with PD were examined. We tried to detect periodontitis causative bacteria in peritoneal dialysis from patients. However, P. gingivalis which is a periodontitis causing bacterium could not be detected.
Next, rat C5a and C5adesArg were prepared in order to clarify in the model animals that bacterial infection and elevation of the concentration of anaphylatoxin directly exacerbate the inflammatory response. When C5a and C5adesArg were administered to rats, a marked inflammatory response was not detected with anaphylatoxin alone. As contrast, C5a administration with low concentration of LPS induced a strong inflammatory response. Interestingly, C5adesArg, that is inactivated C5a also enhanced inflammatory response when pre-administration of low concentration LPS.

Research Products

• [Journal Article] Complement component 5 promotes lethal thrombosis (2017)
  • Author(s): Tomohiro Mizuno, Kengo Yoshioka, Masashi Mizuno, Mie Shimizu, Fumihiko Nagano, Tomoyuki Okuda, Naotake Tsuboi, Shoichi Maruyama, Tadashi Nagamatsu, Masaki Imai
  • Journal Title: Complement component 5 promotes lethal thrombosis. Volume: 16 Issue: 1 Pages: 42714-42714
  • DOI: 10.1038/srep42714
  • Peer Reviewed / Open Access
• [Journal Article] C5a inhibitor protects ischemia/reperfusion injury in rat small intestine. (2015)
  • Author(s): Tuboly E, Futakuchi M, Varga G, Erces D, Tokes T, Meszaros A, Kaszaki J, Suzui M, Imai M, Okada A, Okada N, Boros M, Okada H.
  • Journal Title: Microbiology and Immunology, Epub Volume: 60 Issue: 1 Pages: 35-46
  • DOI: 10.1111/1348-0421.12338
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy. (2015)
  • Author(s): Sei Y, Mizuno M, Suzuki Y, Imai M, Higashide K, Harris CL, Sakata F, Iguchi D, Fujiwara M, Kodera Y, Maruyama S, Matsuo S, Ito Y
  • Journal Title: Molecular Immunology Volume: 153 Issue: 2 Pages: 302-309
  • DOI: 10.1016/j.molimm.2015.02.005
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Expression of membrane complement regulators in patients dependent on peritoneal dialysis therapy and regulation of complement activation in peritoneal dialysate. (2017)
  • Author(s): Mizuno M， Sei Y， Kobayashi K， Shigemoto E， Suzuki Y，Sakata F， Imai M， Maruyama S， Matsuo S, Ito Y.
  • Organizer: The 8th Asia Pacific Chapter Meeting of International Society for Peritoneal Dialysis
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] C5adesArg still induce the acute inflammatory response. (2016)
  • Author(s): Imai M, Odanaka M, Takayama S, Ohta R, Yamazaki S.
  • Organizer: The Cold Spring Harbor Asia conference on Frontiers of Immunology in Health & Disease
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2016-10-03
  • Int'l Joint Research
• [Presentation] C5 promotes histone-induced lethal thromboembolism. (2016)
  • Author(s): Mizuno M, Yoshioka K, Mizuno M, Tsuboi N, Maruyama S, Nagamatsu T, Imai M.
  • Organizer: The XXVI International Complement Workshop
  • Place of Presentation: ホテル日航金沢（石川県・金沢市）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] C5adesArg still induce the acute inflammatory response. (2016)
  • Author(s): Imai M, Odanaka M, Takayama S, Ohta R, Yamazaki S.
  • Organizer: The XXVI International Complement Workshop
  • Place of Presentation: ホテル日航金沢（石川県・金沢市）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] 好中球エラスターゼによる新規カルボキシペプチダーゼRの生成 (2016)
  • Author(s): 河村剛至, 太田里永子, 山本博之, 今井 優樹, 松浦凌太, 松山悟, 佐古兼一,羽二生久夫, 岡田秀親, 松田佳和
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜他(横浜市)
  • Year and Date: 2016-03-26
• [Presentation] Differential roles of complement anaphylatoxin C5a and C5adesArg. (2015)
  • Author(s): Takayama S, Odanaka M, Ohta R, Imai M, Yamazaki S
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2015-11-18
• [Presentation] Foxp3＋CD4＋ regulatory T cells with decreased motility accumulate in aged lymphoid tissue. (2015)
  • Author(s): Yamazaki S, Imai M, Nishioka A, Odanaka M, Morita A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2015-11-18
• [Presentation] Phenotype analysis of Ultra Violet B (UVB)-expanded Foxp3＋CD4＋ regulatory T cells in the skin. (2015)
  • Author(s): Odanaka M, Yamazaki S, Nishioka A, Takayama S, Imai M, Morita A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2015-11-18
• [Presentation] Odanaka M, Yamazaki S, Nishioka A, Kasuya S, Takayama S, Imai M, Morita A. Phenotype analysis of Foxp3＋CD4＋regulatory T cells and dendritic cells in the skin after Ultraviolet B exposure. (2015)
  • Author(s): Odanaka M, Yamazaki S, Nishioka A, Kasuya S, Takayama S, Imai M, Morita A.
  • Organizer: 14th International Workshop on Langerhans Cells
  • Place of Presentation: Kyoto International Community House (Kyoto)
  • Year and Date: 2015-11-05
  • Int'l Joint Research
• [Presentation] Analysis of C5a receptor expression on the various subsets of dendritic cells. (2015)
  • Author(s): Tsujimura K, Ohta R, Imai M, Yamazaki S.
  • Organizer: 14th International Workshop on Langerhans Cells
  • Place of Presentation: Kyoto International Community House (Kyoto)
  • Year and Date: 2015-11-05
  • Int'l Joint Research
• [Presentation] C5a-C5a受容体を標的にした創薬の展望 (2015)
  • Author(s): 今井優樹, 岡田秀親
  • Organizer: 第52回日本補体学会
  • Place of Presentation: 名古屋大学医学部 (名古屋)
  • Year and Date: 2015-08-21
  • Invited
• [Presentation] 好中球エラスターゼにより活性化する新規カルボキシペプチダーゼＲの解析 (2015)
  • Author(s): 河村剛至、太田里永子、今井優樹、大澤真以、今井由美、塩見友祐、賀来美和子、佐古兼一、羽二生久夫、岡田秀親、岡田則子、松田佳和
  • Organizer: 第52回日本補体学会
  • Place of Presentation: 名古屋大学医学部 (名古屋)
  • Year and Date: 2015-08-21
• [Presentation] 線溶抑制を起こさずに炎症を抑制する変異型proCPR作製の試み (2015)
  • Author(s): 河村剛至、太田里永子、今井優樹、大澤真以、今井由美、塩見友祐、賀来美和子、佐古兼一、羽二生久夫、松田佳和
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学他(神戸市)
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] The production of a recombinant bispecific monoclonal antibody against both the complement inhibitor CD59 and the tumor-associated antigen MUC1 (2014)
  • Author(s): Ohta R, Imai M
  • Organizer: 第43回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] 好中球エラスターゼによるプロカルボキシペプチダーゼＲ活性化機構の解析 (2014)
  • Author(s): 河村剛至、大澤真以、今井由美、松田佳和 、太田里永子、今井優樹、羽二生久夫、岡田則子、岡田秀親
  • Organizer: 第51回補体シンポジウム
  • Place of Presentation: 神戸常盤大学 (神戸市)
  • Year and Date: 2014-08-22 – 2014-08-23