| Project/Area Number |
26462866
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
NAKAO Ryoma 国立感染症研究所, 細菌第一部, 主任研究官 (10370959)
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| Co-Investigator(Renkei-kenkyūsha) |
SENPUKU Hidenobu 国立感染症研究所, 細菌第一部, 室長 (20250186)
OHNISHI Makoto 国立感染症研究所, 細菌第一部, 部長 (10233214)
TAKAHASHI Hideyuki 国立感染症研究所, 細菌第一部, 主任研究官 (60321866)
OGATA Yorimasa 日本大学松戸, 歯学部, 教授 (90204065)
HIGUCHI Hideo 東京大学, 理学部, 教授 (90165093)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯周病 / 免疫 / 外膜ヴェシクル / 粘膜免疫 |
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| Outline of Final Research Achievements |
Gram-negative bacteria produce and release spherical, microstructural bodies called outer membrane vesicles (OMVs) during growth. As with other Gram-negative bacteria, OMVs are naturally produced by Porphyromonas gingivalis (Pg). In the present study, we identified immunodominant antigens recognized in serum samples from mice intranasally immunized with PgOMV by a sub-immunoproteome approach with the combination of two-dimensional gel electrophoresis and mass spectrometry analysis. In addition, we discovered a novel approach for purification and selective capture of PgOMV. PgOMV was found to bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner.
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