| Project/Area Number |
26462868
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
根本 英二 東北大学, 歯学研究科, 准教授 (40292221)
島内 英俊 東北大学, 歯学研究科, 名誉教授 (70187425)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯乳頭細胞 / 細胞外Ca2+ / FGF-2 / 歯髄細胞 / 細胞外カルシウム / BMP-2 / 象牙芽細胞 / プロスタグランジンE2 |
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| Outline of Final Research Achievements |
We have previously reported that elevated extracellular Ca2+ increased bone morphogenetic protein-2 (BMP-2) expression in human dental pulp (hDP) cells. In the present study, we examined the effect of extracellular Ca2+ on FGF-2 gene expression in immortalized mouse dental papilla cells (mDP). Extracellular Ca2+ increased FGF-2 gene expression in mDP. Gene expression of calcium-sensing receptor and G protein-coupled receptor family C group 6 member A, both of which are known to be sensors for extracellular Ca2+, was not detected in mDP. Ca2+-mediated Fgf-2 gene expression was reduced by pretreatment with H-89, a protein kinase A (PKA) inhibitor, or PD98059, an ERK1/2 inhibitor, but not by pretreatment with GF-109203X, a PKC inhibitor, or SB203580, a p38 inhibitor. These findings indicate that elevated extracellular Ca2+ increased Fgf-2 gene expression through PKA and ERK1/2 in mDP cells, and that this mechanism may assist in designing regenerative therapies for dentin.
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