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Extracellular calcium increases gene expression of fibroblast growth factor-2 via a PKA and ERK1/2 pathway in mouse dental papilla cells.

Research Project

Project/Area Number 26462868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

Kanaya Sousuke  東北大学, 歯学研究科, 助教 (80375097)

Co-Investigator(Kenkyū-buntansha) 根本 英二  東北大学, 歯学研究科, 准教授 (40292221)
島内 英俊  東北大学, 歯学研究科, 名誉教授 (70187425)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords歯乳頭細胞 / 細胞外Ca2+ / FGF-2 / 歯髄細胞 / 細胞外カルシウム / BMP-2 / 象牙芽細胞 / プロスタグランジンE2
Outline of Final Research Achievements

We have previously reported that elevated extracellular Ca2+ increased bone morphogenetic protein-2 (BMP-2) expression in human dental pulp (hDP) cells. In the present study, we examined the effect of extracellular Ca2+ on FGF-2 gene expression in immortalized mouse dental papilla cells (mDP). Extracellular Ca2+ increased FGF-2 gene expression in mDP. Gene expression of calcium-sensing receptor and G protein-coupled receptor family C group 6 member A, both of which are known to be sensors for extracellular Ca2+, was not detected in mDP. Ca2+-mediated Fgf-2 gene expression was reduced by pretreatment with H-89, a protein kinase A (PKA) inhibitor, or PD98059, an ERK1/2 inhibitor, but not by pretreatment with GF-109203X, a PKC inhibitor, or SB203580, a p38 inhibitor. These findings indicate that elevated extracellular Ca2+ increased Fgf-2 gene expression through PKA and ERK1/2 in mDP cells, and that this mechanism may assist in designing regenerative therapies for dentin.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Metabotropic glutamate receptor 1 promotes cementoblast proliferation via MAP kinase signaling pathways.2016

    • Author(s)
      Kanaya S, Komatsu H, Shimauchi H, Nemoto E.
    • Journal Title

      Connect Tissue Res.

      Volume: 57 Issue: 5 Pages: 417-426

    • DOI

      10.1080/03008207.2016.1195826

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 高濃度細胞外カルシウム刺激に対する間葉系未分化細胞の反応性の解析~fibroblast growth factor 2およびbone morphogenetic protein 2の発現誘導~2016

    • Author(s)
      肖ヒンロ、金谷聡介、向阪幸彦、須藤瑞樹、齋藤正寛、根本英二
    • Organizer
      第59回秋季日本歯周病学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2016-10-07
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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