• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Microbiota profiling of infected root canals using metagenomic analysis

Research Project

Project/Area Number 26462869
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionNiigata University (2016-2017)
Tohoku University (2014-2015)

Principal Investigator

Sato Takuichi  新潟大学, 医歯学系, 教授 (10303132)

Co-Investigator(Kenkyū-buntansha) 真柳 弦  東北大学, 歯学研究科, 助教 (10451600)
河村 好章  愛知学院大学, 薬学部, 教授 (80262757)
田中 香お里  岐阜大学, 学内共同利用施設等, 助手 (20242729)
松山 順子  新潟大学, 医歯学系, 助教 (30293236)
大島 勇人  新潟大学, 医歯学系, 教授 (70251824)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords歯学 / 口腔細菌 / バイオフィルム / 歯内治療 / メタゲノム / 根尖性歯周炎 / 根尖歯周炎
Outline of Final Research Achievements

Apical periodontitis is an infectious and inflammatory disease of periapical tissues caused by oral bacteria invading the root canal system. In the present study, metagenomic analysis was performed in order to profile the microbiota of infected root canals.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (41 results)

All 2019 2018 2017 2016 2015 2014 2011 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 5 results) Presentation (24 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Book (2 results)

  • [Int'l Joint Research] Forsyth Institute(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Eastman Dental Institute(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Forsyth Institute(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Consideration to health care and gender in Sri Lanka.2019

    • Author(s)
      Gunarathne TGNS, Nakamura M, Aoki H, Taguchi M, Hotta K, Ishida M, Yamazaki Y, Sakai S, Sakagami M, Sato M, Sato T, Yamamoto H, Iwafuchi M
    • Journal Title

      J Health Sci Niigata Univ

      Volume: 16

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Oral health promotion program for fostering self-management of the elderly living in communities2017

    • Author(s)
      Sakashita R, Hamada M, Sato T, Abiko Y, Takami M
    • Journal Title

      Intell Autom Soft Comput

      Volume: 23 Issue: 3 Pages: 535-541

    • DOI

      10.1080/10798587.2017.1348459

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling subgingival microbiota of plaque biofilms in the elderly.2016

    • Author(s)
      Abiko Y, Sato T, Sakashita R, Tomida J, Kawamura Y, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 58 Issue: 2 Pages: 62-65

    • DOI

      10.1016/j.job.2015.12.002

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PCR-dipstick DNA chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets.2016

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Mayanagi G, Washio J, Takahashi N
    • Journal Title

      Biomed Res (Tokyo)

      Volume: 37 Pages: 29-36

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microbiota profiling of bronchial fluids of patients with pulmonary carcinoma2015

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Sasaki K, Kondo T, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 57 Issue: 2 Pages: 110-117

    • DOI

      10.1016/j.job.2014.11.001

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] <i>Porphyromonas bronchialis</i> sp. nov. Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer2015

    • Author(s)
      T. Sato, J. Tomida, T. Naka, N. Fujiwara, A. Hasegawa, Y. Hoshikawa, J. Matsuyama, N. Ishida, T. Kondo, K. Tanaka, N. Takahashi, Y. Kawamura
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 237 Issue: 1 Pages: 31-37

    • DOI

      10.1620/tjem.237.31

    • NAID

      130005094770

    • ISSN
      0040-8727, 1349-3329
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oral malodorous gases and oral microbiota: From halitosis to carcinogenesis.2015

    • Author(s)
      Tanda N, Hoshikawa Y, Ishida N, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Journal Title

      J Oral Biosci

      Volume: 57 Issue: 4 Pages: 175-178

    • DOI

      10.1016/j.job.2015.05.004

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Responses of infected dental pulp to αTCP containing antimicrobials in rat molars.2015

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Arch Histol Cytol

      Volume: 75

    • NAID

      130005087018

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Porphyromonas pogonae sp. nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 19882015

    • Author(s)
      Y. Kawamura, S. Kuwabara, S.A. Kania, H. Kato, M. Hamagishi, N. Fujiwara, T. Sato, J. Tomida, K. Tanaka, D.A. Bemis
    • Journal Title

      Syst. Appl. Microbiol.

      Volume: 38 Issue: 2 Pages: 104-109

    • DOI

      10.1016/j.syapm.2014.11.004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rapid and sensitive PCR-dipstick DNA chromatograohy for multiplex analysis of oral microbiota.2014

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Tanner ACR, Takahashi N.
    • Journal Title

      BioMed Res Int

      Volume: 2014 Pages: 180323-180323

    • DOI

      10.1155/2014/180323

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Detection and identification of oral anaerobes from intraoperative bronchial fluids of patients with pulmonary carcinoma2014

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Ishida N, Tanda N, Kawamura Y, Kondo T, Takahashi N
    • Journal Title

      Microbiol Immunol

      Volume: 58(in press) Issue: 7 Pages: 375-381

    • DOI

      10.1111/1348-0421.12157

    • NAID

      40020130159

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] <b>Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars</b>2011

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Archives of Histology and Cytology

      Volume: 73 Issue: 4+5 Pages: 165-175

    • DOI

      10.1679/aohc.73.165

    • NAID

      130005087018

    • ISSN
      0914-9465, 1349-1717
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Microbiota profiling at the mouth of plastic bottles after drinking straight from bottles2018

    • Author(s)
      Sano H, Aida A, Vidanapathirana GU, Wakui A, Hirabuki Y, Takenaka Y, Kawachi M, Aihara H, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
    • Organizer
      International Symposium for Multimodal Research and Education in IOHS-Liaison 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ペットボトルの口の部分に付着する細菌および飲料物中の細菌の量および構成の解析(第2報)2018

    • Author(s)
      河内美帆,佐野拓人,涌井杏奈,平吹有香,曾田彩花,竹中佑太,米田茜音,中畑那奈,相原瞳,Vidanapathirana GU,鷲尾純平,安彦友希,石黒和子,真柳 弦,高橋信博,佐藤拓一
    • Organizer
      第7回口腔保健用機能性食品研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 液体ミルクの安全性の立証に向けて:飲料物の飲み口に付着する細菌の検出および同定2017

    • Author(s)
      涌井杏奈,佐野拓人,平吹有香,曾田彩花,佐藤拓一
    • Organizer
      第12回日本臨床検査学教育学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Quantification and composition of remaining bacteria in plastic bottles after drinking2017

    • Author(s)
      Sano H, Hirabuki Y, Wakui A, Aida A, Abiko Y, Yamaki K, Mayanagi G, Washio J, Takahashi N, Sato T
    • Organizer
      第59回 歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 液体ミルクの安全性の立証に向けて:飲料物の飲み口に付着する細菌の量および構成の解析2017

    • Author(s)
      涌井杏奈,平吹有香,佐野拓人,曾田彩花,佐藤拓一
    • Organizer
      第57回日本臨床化学会年次学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ペットボトル飲料物の飲み口に付着する細菌の検出および同定2017

    • Author(s)
      平吹有香,涌井杏奈,佐野拓人,曾田彩花,竹中佑太,河内美帆,佐藤拓一
    • Organizer
      第54回日本細菌学会中部支部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of a life enhancement program focusing on dining for senior home care patients: A pilot study2017

    • Author(s)
      Sakashita R, Hamada M, Izumoto M, Ono K, Takami M, Ono H, Sato T
    • Organizer
      International Nursing Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 液体ミルクの安全性の立証に向けて:細菌学的解析2017

    • Author(s)
      佐野拓人,涌井杏奈,平吹有香,曾田彩花,竹中佑太,河内美帆,佐藤拓一
    • Organizer
      新大産学交流フェスタ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] A microbiological study on bacteria in the PET bottles after drinking2017

    • Author(s)
      Aida A, Sano H, Wakui A, Hirabuki Y, Takenaka Y, Kawachi M, Vidanapathirana GU, Washio J, Abiko Y, Mayanagi G, Ishiguro K, Yamaki K, Takahashi N, Sato T
    • Organizer
      第65回JADR(国際歯科研究学会日本部会)総会・学術大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ペットボトルの口の部分に付着する細菌の量および構成(A pilot study)2017

    • Author(s)
      曾田彩花、佐藤拓一、石黒和子、安彦友希、真柳 弦、鷲尾純平、高橋信博
    • Organizer
      第6回口腔保健用機能性食品研究会
    • Place of Presentation
      鶴見大学会館(横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 歯科感染症から分離されたグラム陽性嫌気性菌の薬剤感受性2016

    • Author(s)
      田中香お里,佐藤拓一,八巻惠子,林 将大,河村好章
    • Organizer
      第64回日本化学療法学会西日本支部総会
    • Place of Presentation
      沖縄コンベンションセンター(那覇市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Acetone analysis in perioperative patients of lung cancer under oral hygiene program2016

    • Author(s)
      Tanda N, Hoshikawa Y, Sato T, Hosokawa R, Takahashi N, Koseki T
    • Organizer
      MASCC/ISOO 2016
    • Place of Presentation
      Adelaide, Australia
    • Year and Date
      2016-06-23
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] PCR-dipstick DNA chromatography for multiplex and semi-quantitative analysis of plaque biofilm microbiota.2016

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Mayanagi G, Abiko Y, Washio J, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 6th International Symposium for Interface Oral Health Science
    • Place of Presentation
      仙台市
    • Year and Date
      2016-01-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] メタボローム解析:口腔疾患の発生機序解明への新たなアプローチ2015

    • Author(s)
      鷲尾純平,小川珠生,北村 淳,森島浩允,Tian Lingyang,石黒和子,真柳 弦,安彦友希,佐藤拓一,高橋信博
    • Organizer
      第68回東北大学歯学会
    • Place of Presentation
      仙台市
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Malodorous gases from saliva of perioperative patients of lung resection.2015

    • Author(s)
      Tanda N, Ishida N, Takahashi N, Sato T, Hoshikawa Y, Hosokawa R, Koseki T
    • Organizer
      The 63rd Annual Meeting of the Japanese Association for Dental Research
    • Place of Presentation
      福岡市
    • Year and Date
      2015-10-31
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 嫌気性無芽胞グラム陽性桿菌Olsenella spp.の薬剤感受性2015

    • Author(s)
      田中香お里,佐藤拓一,八巻惠子,林 将大,河村好章
    • Organizer
      第63回日本化学療法学会西日本支部総会
    • Place of Presentation
      奈良市
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Profiling of microbiota from infected root canals with and without marginal leakage using anaerobic culture and molecular biological techniques (16S rRNA gene sequencing).2015

    • Author(s)
      Sato T, Yamaki K, Kawamura Y, Tomida J, Tian L, Ishida N, Takeuchi Y, Hashimoto K, Abiko Y, Matsuyama J, Takahashi N
    • Organizer
      The 25th ECCMID (European Congress of Clinical Microbiology and Infectious Diseases)
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2015-04-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Antimicrobial susceptibility profiles of Helicobacter cinaedi strains isolated from patients in Japan.2015

    • Author(s)
      Tomida J, Morita Y, Sato T, Kawamura Y
    • Organizer
      The 25th ECCMID (European Congress of Clinical Microbiology and Infectious Diseases)
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2015-04-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] PCR-dipstick DNA chromatography for semi-quantitative analysis of oral microbiota.2015

    • Author(s)
      Tian L, Sato T, Niwa K, Mayanagi G, Kawase M, Tanner ACR, Takahashi N
    • Organizer
      The 93rd IADR General Session & Exhibition
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Cultivable anaerobic microbiota of infected root canals with/without marginal leakage.2015

    • Author(s)
      Yamaki K, Sato T, Ishida N, Tian L, Hashimoto K, Shimauchi H, Takahashi N
    • Organizer
      The 93rd IADR General Session & Exhibition
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-13
    • Related Report
      2014 Research-status Report
  • [Presentation] A novel method for oral microbiota analysis: PCR-dipstick DNA chromatography.2014

    • Author(s)
      Tian L, Sato T, Niwa K, Mayanagi G, Kawase M, Tanner ACR, Takahashi N
    • Organizer
      China-Japan-Korea Dental Science Symposium
    • Place of Presentation
      Dalian, China
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Responses of infected dental pulp to capping with a mixture of three antibacterial drugs (3Mix) or calcium hydroxide cement in mouse molars.2014

    • Author(s)
      Quispe-Salcedo A, Sato T, Matsuyama J, Ohshima H
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] PCR-dipstick DNA chromatography for multiplex analysis of oral microbiota.2014

    • Author(s)
      Tian L, Sato T, Niwa K, Mayanagi G, Yamaki K, Kawase M, Tanner ACR, Takahashi N
    • Organizer
      第56回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Porphyromonas pogonae sp. nov., a strong beta-hemolytic, low concentration oxygen tolerant species from lizards and human clinical specimens.2014

    • Author(s)
      Kawamura Y, Kuwabara S, Kania SA, Kato H, Hamagishi M, Hayakawa S, Fujiwara N, Naka T, Sato T, Tomida J, Tanaka K, Yoshida Y, Morita Y, Bemis DA
    • Organizer
      The 114th General Meeting of ASM
    • Place of Presentation
      Boston, USA
    • Year and Date
      2014-05-20
    • Related Report
      2014 Research-status Report
  • [Book] Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent2015

    • Author(s)
      Sato T, Kawamura Y, Yamaki K, Ishida N, Tian L, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matsuyama J, Takahashi N
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Book] Interface Oral Health Science 2014: Innovative Research on Biosis-Abiosis Intelligent2015

    • Author(s)
      Sakashita R, Takami M, Ono H, Nishihira T, Sato T, Hamada M
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-06-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi