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In situ analysis of temporal and spatial dynamics of oral biofilm

Research Project

Project/Area Number 26462878
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

Asahi Yoko  大阪大学, 歯学研究科, 助教 (50456943)

Co-Investigator(Kenkyū-buntansha) 野杁 由一郎  新潟大学, 医歯学系, 教授 (50218286)
三浦 治郎  大阪大学, 歯学部附属病院, 助教 (70437383)
恵比須 繁之  大阪大学, 歯学研究科, 特任教授 (50116000)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsバイオフィルム / in situバイオフィルムモデル / 歯科 / 歯内治療学 / 歯学
Outline of Final Research Achievements

Using the in situ biofilm model that reproduces supragingival biofilm, we investigated the temporal dynamics of biofilm-forming bacterial species during the dental biofilm formation. As a results, the initial population of facultative anaerobic bacteria was replaced with the population of gram-negative anaerobic bacteria during dental biofilm formation. Also, in a method using an ionic liquid that is a new scanning electron microscopy observation method, ionic liquids suitable for biofilm observation was evaluated.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (7 results)

  • [Journal Article] Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms2016

    • Author(s)
      Wake N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Gotoh K, Miura J, Machi H, Iida T, Ebisu S.
    • Journal Title

      npj Biofilms and Microbiome

      Volume: 2 Issue: 1 Pages: 16018-16018

    • DOI

      10.1038/npjbiofilms.2016.18

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] メタゲノム解析による実験的in situデンタルバイオフィルム構成細菌の包括的同定2016

    • Author(s)
      和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
    • Journal Title

      Bacterial Adherence & Biofilm

      Volume: 印刷中

    • Related Report
      2015 Research-status Report
  • [Journal Article] Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids.2015

    • Author(s)
      Asahi Y, Miura J, Tsuda T, Kuwabata S, Tsunashima K, Noiri Y, Sakata T, Ebisu S, Hayashi M
    • Journal Title

      AMB Express

      Volume: 5 Pages: 6-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of polysaccharide synthesis by the sinR orthologue PGN_0088 is indirectly associated with the penetration of Porphyromonas gingivalis biofilms by macrolide antibiotics.2015

    • Author(s)
      Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Ebisu S, Hayashi M
    • Journal Title

      Microbiology

      Volume: 161 Issue: 2 Pages: 422-429

    • DOI

      10.1099/mic.0.000013

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms2014

    • Author(s)
      Asahi Y. et al.
    • Journal Title

      Jouranl of Applied Microbiology

      Volume: 116 Issue: 5 Pages: 1164-1171

    • DOI

      10.1111/jam.12458

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 過剰根管充填された難治性根尖性歯周炎の治療経過における細菌学的解析2016

    • Author(s)
      永山智崇,朝日陽子,住岡龍一,米田直道,松井沙織,後藤満帆,野杁由一郎,恵比須繁之,林 美加子
    • Organizer
      第145回日本歯科保存学会秋季学術大会
    • Place of Presentation
      キッセイ文化ホール(長野県松本市)
    • Year and Date
      2016-10-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 難治性根尖性歯周炎において根尖孔外バイオフィルム以外の原因の関与が考えられた1症例2016

    • Author(s)
      永山智崇、朝日陽子、住岡龍一、米田直道、野杁由一郎、恵比須繁之、林美加子
    • Organizer
      第144回日本歯科保存学会2016年度春季学術大会
    • Place of Presentation
      栃木県総合文化センター(栃木県宇都宮市)
    • Year and Date
      2016-06-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 実験的in situデンタルバイオフィルム構成細菌の包括的解析2015

    • Author(s)
      和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
    • Organizer
      第143回日本歯科保存学会
    • Place of Presentation
      文京シビックホール(東京都文京区)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
  • [Presentation] メタゲノム解析による実験的in situデンタルバイオフィルム構成細菌の包括的同定2015

    • Author(s)
      和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
    • Organizer
      第29回日本バイオフィルム学会
    • Place of Presentation
      ホテル竹島(愛知県蒲郡市)
    • Year and Date
      2015-07-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Time-dependent Analysis of Dental Biofilms by New in situ Model2015

    • Author(s)
      Wake N, Asahi Y, Noiri Y, Machi H, Ebisu S, Hayashi M
    • Organizer
      93th IADR
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Quantitative Analysis of Human Dental Biofilms by New in situ Model2014

    • Author(s)
      Wake N, Asahi Y, Noiri Y, Machi H, Ebisu S, Hayashi M
    • Organizer
      JADR
    • Place of Presentation
      大阪
    • Year and Date
      2014-12-04
    • Related Report
      2014 Research-status Report
  • [Presentation] デンタルバイオフィルムの形成と制御に関する包括的 in situ 解析~各細菌属の経時的定量解析2014

    • Author(s)
      和氣菜々子, 朝日 陽子, 町博之, 野杁 由一郎, 恵比須 繁之, 林美加子
    • Organizer
      第141回 日本歯科保存学会
    • Place of Presentation
      山形
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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