In situ analysis of temporal and spatial dynamics of oral biofilm

• Project/Area Number: 26462878
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: Asahi Yoko 大阪大学, 歯学研究科, 助教 (50456943)
• Co-Investigator(Kenkyū-buntansha): 野杁 由一郎 新潟大学, 医歯学系, 教授 (50218286) ; 三浦 治郎 大阪大学, 歯学部附属病院, 助教 (70437383) ; 恵比須 繁之 大阪大学, 歯学研究科, 特任教授 (50116000)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バイオフィルム / in situバイオフィルムモデル / 歯科 / 歯内治療学 / 歯学

Outline of Final Research Achievements

Using the in situ biofilm model that reproduces supragingival biofilm, we investigated the temporal dynamics of biofilm-forming bacterial species during the dental biofilm formation. As a results, the initial population of facultative anaerobic bacteria was replaced with the population of gram-negative anaerobic bacteria during dental biofilm formation. Also, in a method using an ionic liquid that is a new scanning electron microscopy observation method, ionic liquids suitable for biofilm observation was evaluated.

Research Products

• [Journal Article] Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms (2016)
  • Author(s): Wake N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Gotoh K, Miura J, Machi H, Iida T, Ebisu S.
  • Journal Title: npj biofilm and microbiomes Volume: 2 Pages: 16018-16018
  • DOI: 10.1038/npjbiofilms.2016.18
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] メタゲノム解析による実験的in situデンタルバイオフィルム構成細菌の包括的同定 (2016)
  • Author(s): 和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
  • Journal Title: Bacterial Adherence & Biofilm Volume: 印刷中
• [Journal Article] Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids. (2015)
  • Author(s): Asahi Y, Miura J, Tsuda T, Kuwabata S, Tsunashima K, Noiri Y, Sakata T, Ebisu S, Hayashi M
  • Journal Title: AMB Express Volume: 5 Pages: 6-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibition of polysaccharide synthesis by the sinR orthologue PGN_0088 is indirectly associated with the penetration of Porphyromonas gingivalis biofilms by macrolide antibiotics. (2015)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Ebisu S, Hayashi M
  • Journal Title: Microbiology Volume: 161 Pages: 422-429
  • DOI: 10.1099/mic.0.000013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms. (2014)
  • Author(s): Asahi Y. et al.
  • Journal Title: Journal of applied microbiology Volume: 116 Pages: 1164-1171
  • DOI: 10.1111/jam.12458
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 過剰根管充填された難治性根尖性歯周炎の治療経過における細菌学的解析 (2016)
  • Author(s): 永山智崇，朝日陽子，住岡龍一，米田直道，松井沙織，後藤満帆，野杁由一郎，恵比須繁之，林 美加子
  • Organizer: 第145回日本歯科保存学会秋季学術大会
  • Place of Presentation: キッセイ文化ホール（長野県松本市）
  • Year and Date: 2016-10-28
• [Presentation] 難治性根尖性歯周炎において根尖孔外バイオフィルム以外の原因の関与が考えられた１症例 (2016)
  • Author(s): 永山智崇、朝日陽子、住岡龍一、米田直道、野杁由一郎、恵比須繁之、林美加子
  • Organizer: 第144回日本歯科保存学会2016年度春季学術大会
  • Place of Presentation: 栃木県総合文化センター（栃木県宇都宮市）
  • Year and Date: 2016-06-10
• [Presentation] 実験的in situデンタルバイオフィルム構成細菌の包括的解析 (2015)
  • Author(s): 和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
  • Organizer: 第143回日本歯科保存学会
  • Place of Presentation: 文京シビックホール（東京都文京区）
  • Year and Date: 2015-11-13
• [Presentation] メタゲノム解析による実験的in situデンタルバイオフィルム構成細菌の包括的同定 (2015)
  • Author(s): 和氣菜々子, 朝日陽子, 町博之, 野杁由一郎, 恵比須繁之, 林美加子
  • Organizer: 第29回日本バイオフィルム学会
  • Place of Presentation: ホテル竹島（愛知県蒲郡市）
  • Year and Date: 2015-07-11
• [Presentation] Time-dependent Analysis of Dental Biofilms by New in situ Model (2015)
  • Author(s): Wake N, Asahi Y, Noiri Y, Machi H, Ebisu S, Hayashi M
  • Organizer: 93th IADR
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-13
• [Presentation] Quantitative Analysis of Human Dental Biofilms by New in situ Model (2014)
  • Author(s): Wake N, Asahi Y, Noiri Y, Machi H, Ebisu S, Hayashi M
  • Organizer: JADR
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-04
• [Presentation] デンタルバイオフィルムの形成と制御に関する包括的 in situ 解析～各細菌属の経時的定量解析 (2014)
  • Author(s): 和氣菜々子, 朝日 陽子, 町博之, 野杁 由一郎, 恵比須 繁之, 林美加子
  • Organizer: 第１４１回 日本歯科保存学会
  • Place of Presentation: 山形
  • Year and Date: 2014-10-31