Multi-faceted analysis in acid resistance and remineralization of root dentin and establishment of clinical evaluation method

• Project/Area Number: 26462879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: IWAMI Yukiteru 大阪大学, 歯学研究科, 招へい教員 (90303982)
• Co-Investigator(Kenkyū-buntansha): 林 美加子 大阪大学, 歯学研究科, 教授 (40271027) ; 山本 洋子 大阪大学, 歯学研究科, 招へい教員 (60448107)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: フッ素 / 定量測定法 / う蝕 / 加速器 / PIXE/PIGE / 脱灰 / カルシウム / う蝕評価

Outline of Final Research Achievements

The evaluation method of caries due to the change of calcium using In-air micro-PIXE / PIGE measurement method developed by us correlated with the decalcification evaluation by micro CT. Although the effect of dental caries control by fluorine contained in the tooth is obvious, it was found that fluorine can be further suppressed for caries by coexisting with calcium.
Clinical evaluation by laser diagnostic equipment, objective color diagnostic method, quantitative photoexcited fluorescence method, etc. has been great influenced of individual difference and environment, and further data accumulation was necessary to summarize relevance. We were able to take the first step in the development of new materials for root caries prevention.

Research Products

• [Journal Article] Efficacy of a new filler-containing root coating material for dentin remineralization (2016)
  • Author(s): Okuyama K, Kadowaki Y, Matsuda Y, Hashimoto N, Oki S, Yamamoto H, Tamaki Y, Sano H
  • Journal Title: American Journal of Dentistry Volume: 29 Pages: 213-218
  • Peer Reviewed
• [Journal Article] The demineralization inhibit effect of fluoride-containing sealants for primary teeth (2016)
  • Author(s): Matsuda Y, Ushimura S, Okuyama K, Komatsu H, Yamamoto H, Hashimoto N, Hayashi M, Nomachi M, Yasuda K, Satoh T, Koka M
  • Journal Title: JAEA Takasaki Annual Report 2014 JAEA-Review 2015 Volume: 22 Pages: 83-83
  • Peer Reviewed
• [Journal Article] Fluoride varnish remaining after physical stress (2016)
  • Author(s): Matsuda Y, Okuyama K, Komatsu H, Yamamoto H, Hashimoto N, Hayashi M, Nomachi M, Yasuda K, Satoh T, Koka M
  • Journal Title: QST Takasaki Annual Report 2015 Volume: なし Pages: 152-152
  • Peer Reviewed
• [Journal Article] 大気マイクロPIXE/PIGEを用いた歯根面象牙質における脱灰のpHによる影響 (2016)
  • Author(s): 奥山克史、山本洋子、松田康裕、八木香子、安田啓介、鈴木耕拓、林美加子、玉置幸道、斎藤隆史、能町正治、菅谷頼仁
  • Journal Title: 若狭湾エネルギー研究センター研究年報（平成２７年度） Volume: 18 Pages: 76-77
  • Peer Reviewed
• [Journal Article] The demineralization inhibit effect of fluoride-containing sealants for primary teeth (2016)
  • Author(s): Matsuda Y, Ushimura S, Okuyama K, Komatsu H, Yamamoto H, Hashimoto N, Hayashi M, Nomachi M, Yasuda K, Satoh T, Koka M
  • Journal Title: JAEA Takasaki Annual Report 2014 JAEA-Review Volume: 2015-022 Pages: 83-83
  • Peer Reviewed / Open Access
• [Journal Article] Tooth-bound fluoride uptake from fluoride-containing dental materials (2015)
  • Author(s): Komatsu H, Okuyama K, Yamamoto H, Yasuda K, Nomachi M, Sugaya Y
  • Journal Title: International Journal of PIXE Volume: 23 Issue: 03n04 Pages: 127-134
  • DOI: 10.1142/s0129083513410052
  • Peer Reviewed
• [Journal Article] Fluorine uptake into the human enamel surface from fluoride-containing sealing materials during cariogenic pH cycling (2015)
  • Author(s): Matsuda Yasuhiro, Okuyama Katsushi, Yamamoto Hiroko, Komatsu Hisanori, Koka Masashi, Sato Takahiro, Hashimoto Naoki, Oki Saiko, Kawamoto Chiharu, Sano Hidehiko
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Volume: 348 Pages: 156-159
  • DOI: 10.1016/j.nimb.2015.01.062
  • Peer Reviewed / Open Access
• [Journal Article] A new technique for analyzing trace element uptake by human enamel (2015)
  • Author(s): Funato Y, Matsuda Y, Okuyama K, Yamamoto H, Komatsu H, Sano H
  • Journal Title: Dental Materials Journal Volume: 34 Issue: 2 Pages: 240-245
  • DOI: 10.4012/dmj.2014-127
  • NAID: 130005062410
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 歯根面象牙質における脱灰評価へのマイクロPIXE/PIGEの応用 (2015)
  • Author(s): 奥山克史、山本洋子、松田康裕、小松久憲、佐野英彦、安田啓介、鈴木耕拓、林美加子、能町正治、菅谷頼仁
  • Journal Title: 若狭湾エネルギー研究センター研究年報（平成２６年度） Volume: 17 Pages: 31-32
  • Peer Reviewed / Open Access
• [Journal Article] フッ化物含有う蝕予防材による脱灰抑制効果のマイクロPIXE/PIGEによる評価 (2015)
  • Author(s): 松田康裕、奥山克史、山本洋子、小松久憲、江夏昌志、佐藤隆博、佐野英彦、安田啓介、鈴木耕拓、林美加子、能町正治、菅谷頼仁
  • Journal Title: 若狭湾エネルギー研究センター研究年報（平成２６年度） Volume: 17 Pages: 33-34
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of caries progression in dentin treated by fluoride-containing materials using an in-air micro-PIGE and micro-PIXE measurement system (2015)
  • Author(s): H. Yamamoto, Y. Iwami, K. Yagi, M. Hayashi, H. Komatsu, K. Okuyama, Y. Matsuda, K.Yasuda
  • Journal Title: Nuclear Instruments & Methods in Physics Research Volume: B348 Pages: 152-155
  • DOI: 10.1016/j.nimb.2015.01.068
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distribution of fluoride penetrating from fluoride-containing filling materials in pre-demineralized enamel (2015)
  • Author(s): Matsuda Y, Okuyama K, Yamamoto H, Komatsu H, Oki S, Hashimoto N, Iwami Y, Hayashi M, Sano H, Nomachi M, Yasuda K, Satoh T, Koka M
  • Journal Title: JAEA Takasaki Annual Report 2013 JAEA-Review 2014 Volume: 050 Pages: 92-92
  • Peer Reviewed
• [Presentation] 脱核反応による歯質中のフッ素分布測定13 (2017)
  • Author(s): 松田康裕、斎藤隆史、奥山克史、山本洋子、岩見行晃、林美加子、能町正治、安田啓介、山田尚人、佐藤隆博
  • Organizer: 第１回QST高崎研シンポジウム
  • Place of Presentation: 高崎量子応用研究所（群馬県高崎市）
  • Year and Date: 2017-01-26
• [Presentation] Evaluation of the acid resistance of root dentin when applying fluoride containing materials incorporating Ca using in-air micro-PIXE/PIGE (2016)
  • Author(s): Yagi K, Yamamoto H, Uemura R, Okuyama K, Matsuda Y, Suzuki K, Hayashi M
  • Organizer: International dental materials congress 2016
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2016-11-05
  • Int'l Joint Research
• [Presentation] XRD and FT-IR analysis on fluoride-treated hydroxyapatite (2016)
  • Author(s): Okuyama K, Komada Y, Abe M, Matsuda Y, Yamamoto H, Tamaki Y
  • Organizer: International dental materials congress 2016
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2016-11-05
  • Int'l Joint Research
• [Presentation] フッ化物含有知覚過敏抑制材による象牙質表面の脱灰抑制効果 (2016)
  • Author(s): 松田康裕、奥山克史、山本洋子、大木彩子、泉川昌宣、油井知雄、伊藤修一、佐野英彦、斎藤隆史
  • Organizer: 日本歯科保存学会2016年度秋季学術大会（第145回）
  • Place of Presentation: 長野県松本文化会館（長野県松本市）
  • Year and Date: 2016-10-27
• [Presentation] Demineralize Prevention of Dentin With S-PRG Varnish via Automatic pH-cycling (2016)
  • Author(s): Matsuda Y, Okuyama K, Yamamoto H, Oki S, Izumikawa M, Yui T, Ito S, Sano H, Saito T
  • Organizer: 18th Joint scientific meeting of 146th scientific meeting of the Korean Academy of Conservative Dentistry (KACD) and JSCD
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-10-22
  • Int'l Joint Research
• [Presentation] Evaluation of demineralization in root dentin using in-air micro PIXE/PIGE (2016)
  • Author(s): Yagi K, Uemura R, Yamamoto H, Matsuda Y, Okuyama K, Yasuda K, Hayashi M
  • Organizer: 94th General session of IADR
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-06-24
  • Int'l Joint Research
• [Presentation] In-air micro beam PIXE/PIGEによる根面象牙質の耐酸性評価－pHおよびフッ化物の影響に関する検討－ (2016)
  • Author(s): 八木香子、山本洋子、上村玲央、奥山克史、松田康裕、鈴木耕拓、林美加子
  • Organizer: 日本歯科保存学会2016年度春季学術大会（第144回）
  • Place of Presentation: 栃木県総合文化センター（栃木県宇都宮市）
  • Year and Date: 2016-06-10
• [Presentation] フッ化物含有歯科材料によるフッ素のエナメル質への取り込みと結合状態の評価 (2015)
  • Author(s): 奥山克史、松田康裕、山本洋子、橋本直樹、林美加子、佐野英彦、玉置幸道
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 文京シビックホール〔東京都）
  • Year and Date: 2015-11-13
• [Presentation] 核反応による歯質中のフッ素分布測定１２ (2015)
  • Author(s): 松田康裕、斎藤隆史、奥山克史、大木彩子、橋本直樹、佐野英彦、山本洋子、岩見行晃、林美加子、能町正治、安田啓介、山田尚人、喜多村茜、佐藤隆博
  • Organizer: 第１０回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎量子応用研究所（高崎市）
  • Year and Date: 2015-10-09
• [Presentation] In-air micro-beam PIXE/PIGEを用いた根面象牙質の脱灰評価 (2015)
  • Author(s): 八木香子、山本洋子、岩見行晃、林美加子
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 北九州国際会議場（北九州市）
  • Year and Date: 2015-06-25
• [Presentation] 脱灰処理されたエナメル質におけるフッ化物含有材料からのフッ素の拡散 (2014)
  • Author(s): 松田康裕、奥山克史、小松久憲、大木彩子、橋本直樹、佐野英彦、山本洋子、岩見行晃、林美加子、能町正治、安田啓介、山田尚人、喜多村茜、佐藤隆博、江夏昌志
  • Organizer: 第９回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎
  • Year and Date: 2014-10-10
• [Presentation] Fluorine uptake into human enamel surface from fluoride-containing sealing materials during cariogenic pH cycling (2014)
  • Author(s): Y. Matsuda, K. Okuyama , M. Koka, T. Satoh, H. Komatsu and H.Sano
  • Organizer: 14th ICNMTA
  • Place of Presentation: Padova, Italy
  • Year and Date: 2014-07-08
• [Presentation] Evaluation of caries progression in dentin treated by fluoride-containing materials using PIGE/PIXE system (2014)
  • Author(s): H. Yamamoto , Y. Iwami , K. Yagi, M. Hayashi , H. Komatsu , K. Okuyama , Y. Matsuda, and K. Yasuda
  • Organizer: 14th ICNMTA
  • Place of Presentation: Padova, Italy
  • Year and Date: 2014-07-08