Effects of aging on hard tissue formation using Low-intensity pulsed ultrasound and BMP

• Project/Area Number: 26462883
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMAJI KOZO 岡山大学, 大学病院, 講師 (30374531)
• Co-Investigator(Kenkyū-buntansha): 川浪 雅光 北海道大学, 歯学研究科, 名誉教授 (10133761) ; 吉山 昌宏 岡山大学, 医歯薬学総合研究科, 教授 (10201071) ; 菅谷 勉 北海道大学, 歯学研究科, 准教授 (10211301)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 低出力超音波パルス / BMP / 硬組織形成 / 加齢

Outline of Final Research Achievements

This study evaluated the effects of aging on hard tissue formation induced by BMP using Low-intensity pulsed ultrasound（LIPUS）in young and aged rats.
Tertialy dentin formation was promoted by using pulp capping material contained BMP when LIPUS treatment was performed. There was no significant difference between the young and the aged rat in the volume of tertialy dentin. Newly formed bone was promoted by implantation of material contained BMP at palatal subperiosteal site when LIPUS treatment was performed, although the volume of bone decreased with aging. These results suggest that the effects of aging on hard tissue formation might vary with the experimental sites.

Research Products

• [Journal Article] Effects of Aging on Bone Formation Induced by BMP-2 using Low-intensity Pulsed Ultrasound. (2016)
  • Author(s): Kozo YAMAJI, Akihito YOKOYAMA, Kumiko MATSUZAKI, Naoko OHARA, Eriko NISHIYAMA, Tsutomu SUGAYA, Masahiro YOSHIYAMA
  • Journal Title: Journal of Oral Tissue Engineering Volume: 14 Pages: 65-69
  • NAID: 130005277139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of high-frequency electromagnetic wave stimulation on bone repair in rat calvaria defects. (2016)
  • Author(s): Sato T, Sugaya T, Kudo M, Nakatani M, Maeda N, Kawanami M, Tominaga T.
  • Journal Title: Journal of Oral Tissue Engineering Volume: 14 Pages: 59-64
  • NAID: 130005277140
  • Peer Reviewed / Open Access
• [Journal Article] Induction of ES cells from Human Bone Marrow Mesenchymal Stem Cells: Epigenetic Control and Initialization of the Cells, (2014)
  • Author(s): Izawa S, Yamaji K, Hoshika T, Nishitani Y, Yoshiyama M
  • Journal Title: Journal of Oral Tissue Engineering Volume: 11 Pages: 220-226
  • NAID: 130004941114
  • Peer Reviewed / Open Access
• [Presentation] スキャホールドが低出力超音波パルスとBMPを用いた骨形成に及ぼす影響 (2017)
  • Author(s): 山路公造, 横山章人, 大原直子, 松崎久美子, 神農泰生, 吉山昌宏
  • Organizer: 第14回日本再生歯科医学会
  • Place of Presentation: 札幌市
• [Presentation] 高周波電流刺激による骨再生 (2016)
  • Author(s): 佐藤賢人、菅谷 勉、工藤 愛、前田良子、富永敏彦
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-10-21
• [Presentation] 高周波電流刺激の電流値と通電期間が骨再生に及ぼす影響 (2016)
  • Author(s): 佐藤賢人、菅谷 勉、中谷充宣、前田良子、川浪雅光、富永敏彦
  • Organizer: 第144回春季日本歯科保存学会
  • Place of Presentation: 宇都宮市
• [Presentation] 加齢が増殖因子を用いた硬組織形成に及ぼす影響 (2015)
  • Author(s): 横山章人, 山路公造, 伊澤俊次, 田中久美子, 大原直子, 吉山昌宏
  • Organizer: 第13回日本再生歯科医学会学術大会
  • Place of Presentation: 日本歯科大学新潟生命歯学部
  • Year and Date: 2015-08-29
• [Presentation] ヒト脂肪由来幹細胞を用いたES細胞の誘導に関する研究―エピジェネティック制御と細胞の初期化― (2014)
  • Author(s): 伊澤俊次,山路公造,星加知宏,西谷佳浩,吉山昌宏
  • Organizer: 第12回日本再生歯科医学会学術大会
  • Place of Presentation: 徳島大学
  • Year and Date: 2014-08-26