Effects of the analgesic drug for endodontic treatment on odontoblasts.
Project/Area Number |
26462894
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservative dentistry
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Research Institution | Tokyo Dental College |
Principal Investigator |
Shimada Miyuki 東京歯科大学, 歯学部, 非常勤講師 (60297348)
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Co-Investigator(Kenkyū-buntansha) |
渋川 義幸 東京歯科大学, 歯学部, 准教授 (30276969)
佐藤 正樹 東京歯科大学, 歯学部, 講師 (80598855)
木村 麻記 東京歯科大学, 歯学部, 助教 (90582346)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 歯内療法薬 / TRP channel / Odontoblast / Guaiacol / Trigeminal ganglion / TRP channel / 歯内治療薬 / TRP channels / Odontoblasts / trigeminal ganglion / 歯内治療学 |
Outline of Final Research Achievements |
We investigated effects of the guaiacol on odontoblasts which play an important role in nociceptive process via activation of transient receptor potential (TRP) channels during stimulation applied to the dentin surface. Guaiacol evoked Ca2+ influx from the extracellular medium via TRP vanilloid subfamily members-3 channels on the plasma membrane of odontoblasts, while the drug did not inhibit hypotonic-stimulation-induced Ca2+ entry in odontoblasts. These results implied that analgesic effect on odontoblast by guaiacol is unlikely in the dentinal pain. The bradykinin-induced and a direct mechanical stimulation-induced intracellular Ca2+ concentration ([Ca2+]i) increases in trigeminal ganglion (TG) neurons were significantly inhibited by guaiacol. We suggested that guaiacol develops excellent analgesic effect on the pulpitis by inhibiting mechanosensitive ionic channels in TG neurons.
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Report
(5 results)
Research Products
(56 results)
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[Journal Article] Depolarization-induced Intracellular Free Calcium Concentration Increases Show No Desensitizing Effect in Rat Odontoblasts2015
Author(s)
Kojima Y, Higashikawa A, Kimura M, Sato M, Mochizuki H, Ogura K, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
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Journal Title
The Bulletin of Tokyo Dental College
Volume: 56
Issue: 2
Pages: 131-134
DOI
NAID
ISSN
0040-8891
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Transient Receptor Potential Cation Channel Subfamily Vanilloid Member 3 is not Involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells2015
Author(s)
Higashikawa A, Kojima Y, Sato M, Kimura M, Ogura K, Mochizuki H, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M.
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Journal Title
The Bulletin of Tokyo Dental College
Volume: 56
Issue: 4
Pages: 259-262
DOI
NAID
ISSN
0040-8891
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction2015
Author(s)
Shibukawa, Y., Sato, M., Kimura, M., Sobhan, U., Shimada, M., Nishiyama, A., Kawaguchi, A., Soya, M., Kuroda, H., Katakura, A., Ichinohe, T., and Tazaki, M.
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Journal Title
Pflügers Archiv European Journal of Physiology
Volume: 467
Issue: 4
Pages: 843-863
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Odontoblasts express plasma membrane Ca2+-ATPase 1 and 42018
Author(s)
Kimura Maki, Higashikawa Asuka, Kojima Yuki, Terashima-Shigefuji Reiko, Inoue Hiroyuki, Nagai Sayoko, Kamata Satomi, Shimada Miyuki, Kuroda Hidetaka, Ogura Kazuhiro, Mochizuki Hiroyuki, Kouno Kyosuke, Ando Masayuki, Shibukawa Yoshiyuki, Tazaki Masakazu
Organizer
第95回日本生理学会大会
Related Report
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[Presentation] Alkali- and ADP-sensitive store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts.2017
Author(s)
Kimura M., Sato M., Kojima Y., Higashikawa A., Shiozaki Y., Satou R., Shigefuji R., Shimada M., Kuroda H., Ogura K., Mochizuki H., Kouno K., Shibukawa Y., Tazaki M.
Organizer
第94回日本生理学会大会
Place of Presentation
浜松市
Year and Date
2017-03-28
Related Report
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[Presentation] High pH-sensitive TRPA1 channel activation acts as a key Ca2+ signaling that regulates dentin mineralization by odontoblasts.2016
Author(s)
Kimura M., Sato M., Kojima Y., Higashikawa A., Shimada M., Shiozaki Y., Satou R., Shigefuji R., Ogura K., Mochizuki H., Kouno K., Shibukawa Y., Tazaki M.
Organizer
International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
Place of Presentation
Nagoya, Japan
Year and Date
2016-06-26
Related Report
Int'l Joint Research
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[Presentation] Store-operated Ca2+ entry in rat odontoblasts.2016
Author(s)
Kimura M., Higashikawa A., Sato M., Kojima Y., Shimada M., Shiozaki Y., Satou R., Shigefuji R., Ogura K., Mochizuki H., Kouno K., Shibukawa Y., Tazaki M.
Organizer
International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
Place of Presentation
Nagoya, Japan
Year and Date
2016-06-26
Related Report
Int'l Joint Research
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[Presentation] Odontoblasts as sensory receptors: Transient receptor potential channels, pannexin-1 and ionotropic ATP receptors, mediate intracellular odontoblast-trigeminal ganglion neuron signal transduction.2016
Author(s)
Shibukawa Y., Sato M., Kimura M., Sobhan, U., Kojima Y., Higashikawa A., Shiozaki Y., Satou R., Shigefuji R., Shimada M., Ogura K., Mochizuki H., Kouno K., Tazaki M.
Organizer
International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
Place of Presentation
Nagoya, Japan
Year and Date
2016-06-26
Related Report
Int'l Joint Research
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[Presentation] ATP released from odontoblasts by mechanosensitive TRP and piezo channel activations generates P2X3-mediated action potentials in trigeminal ganglion neurons.2016
Author(s)
Sato M., Kimura M., Kojima Y., Higashikawa A., Shiozaki Y., Satou R., Shigefuji R., Shimada M, Ogura K., Mochizuki H., Kouno K., Tazaki M., Shibukawa Y.
Organizer
International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
Place of Presentation
Nagoya, Japan
Year and Date
2016-06-26
Related Report
Int'l Joint Research
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[Presentation] Biophysical properties of a Voltage-dependent K+ current in odontoblasts.2016
Author(s)
Kojima Y., Higashikawa A., Kimura M., Sato M., Shiozaki Y., Satou R., Shigefuji R., Shimada M., Ogura K., Mochizuki H., Kouno K., Shibukawa Y., Tazaki. M.
Organizer
International Association of Dental Research (IADR) Pulp Biology and Regeneration Group (PBRG) Symposium 2016
Place of Presentation
Nagoya, Japan
Year and Date
2016-06-26
Related Report
Int'l Joint Research
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[Presentation] Expression of store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts2016
Author(s)
Kimura M, Sato M, Kojima Y, Higashikawa A, Shiozaki Y, Satou R, Shigefuji R, Shimada M, Kobune K, Ogura K, Mochizuki H, Kouno K, Shibukawa Y, Tazaki M
Organizer
第93回日本生理学会大会
Place of Presentation
札幌市
Year and Date
2016-03-22
Related Report
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[Presentation] Evoked inward currents in trigeminal ganglion neurons caused by intracellular transmitters, ATP, released from mechanically stimulated odontoblasts2016
Author(s)
Sato M, Kobune K, Kimura M, Kojima Y, Higashikawa A, Ogura K, Mochizuki H, Kouno K, Shimada M, Shibukawa Y, Tazaki M.
Organizer
第93回日本生理学会大会
Place of Presentation
札幌市
Year and Date
2016-03-22
Related Report
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[Presentation] Activated mechanisms of H+-sensing ionotropic receptors and G-protein-coupled H+-sensing receptors in odontoblasts2015
Author(s)
Sato M, Kurasawa K, Okabayashi K, Kimura M, Kojima Y, Higashikawa A, Shimada M, Ogura K,Mochizuki H, Sase T, Shinya A, Kobune K, Shibukawa Y and Tazaki M
Organizer
JOINT RESEARCH PROJECT (JSPS) UNDER THE JAPAN-KOREA BASIC SCIENTIFIC COOPERATION
Place of Presentation
Narita, Chiba, Japan
Year and Date
2015-06-12
Related Report
Invited
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[Presentation] Alkaline-sensitive intracellular Ca2+ signaling pathway in rat odontoblasts2015
Author(s)
Kimura M, Sato M, Kojima Y, Higashikawa A, Nishiyama A, Satou R, Shiozaki Y, Shimada M,Ogura K,Mochizuki H, Sase T, Shinya A, Kobune K, Furuya T, Sato T, Shibukawa Y, Tazaki M
Organizer
JOINT RESEARCH PROJECT (JSPS) UNDER THE JAPAN-KOREA BASIC SCIENTIFIC COOPERATION
Place of Presentation
Narita, Chiba, Japan
Year and Date
2015-06-12
Related Report
Invited
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[Presentation] Ca2+ signaling activated by alkaline environment in rat odontoblasts2015
Author(s)
Kimura Maki, Sato Masaki, Kojima Yuki, Higashikawa Asuka, Nishiyama Akihiro, Satou Ryoichi, Shiozaki Yuuta, Shimada Miyuki, Ogura Kazuhiro, Mochizuki Hiroyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
Organizer
第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
Place of Presentation
神戸市
Year and Date
2015-03-21 – 2015-03-23
Related Report
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