| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Sixty three periapical lesions were examined, and fifty periapical granulomas were obtained. Thirty eight out of 50 periapical granulomas involved Epstein-Barr virus (EBV). Six out of 9 samples showed EBER mRNA and LMP-1 protein expressing cells. Six out of 9 samples involved Polphylomonas endodontalis. As determined using high performance liquid chromatography, n-butylic acid was most produced in the culture supernatants of P. endodontalis. EBV-infecte cells were cultured with the culture supernatants of P. endodontalis, and the cells expressed ZEBRA protein in the dose dependent manner.
According to these findings, it is concluded that periapcal granulomas were infected with EBV, and EBV was reactivated by n-butyric acid produced from P. endodontalis.
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