| Project/Area Number |
26462900
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
TOMIYAMA KIYOSHI 神奈川歯科大学, 大学院歯学研究科, 講師 (90237131)
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| Co-Investigator(Kenkyū-buntansha) |
向井 義晴 神奈川歯科大学, 大学院歯学研究科, 教授 (40247317)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | biofilm / tannin / chlorhexidine / persimmon / バイオフィルム / タンニン / クロルヘキシジン / 象牙質 / ポリマイクロバイアルバイオフィルム / 柿 / 抗菌剤 / 縮合型タンニン / ポリマイクロバイアル |
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| Outline of Final Research Achievements |
Pancil PS-M (Rilis Co., Ltd, Osaka, Japan) is a food addictive containing condensed tannin extracted from astringent persimmon (Kaki). Polymicrobial biofilm were cultured on glass plates (diameter: 12 mm, thickness: 0.15 mm) using dilution of stimulated saliva of one healthy adult in a polymicrobial biofilm model.After biofilm formation for 24 h, treatment was conducted as follows: The specimens (12 per group) were immersed for 5 min in: 0.2% chlorhexidine digluconate (CHX) (Corsodyl, GlaxoSmithKline) (group 0.2C); 4.0% Pancil PS-M (PS-M) (group 4P); and deionized water (control group). After treatments, group 4P and 0.2C showed significantly lower CFU than that of control group (cont: 4.30 × 108 CFU/ml, 4P: 1.67 × 107 CFU/ml, 0.2C: 1.74 × 107 CFU/ml). Also, 4P suppressed remarkably regrowth of CFU in biofilms comparing 0.2C even after 48h-regrowth of biofilm (cont: 7.80 × 108, 4P: 1.26 × 107, 0.2C: 1.09 × 108).
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