| Project/Area Number |
26462902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
土井 豊 朝日大学, 歯学部, 名誉教授 (40116067)
近藤 信夫 朝日大学, 歯学部, 教授 (40202072)
川木 晴美 朝日大学, 歯学部, 准教授 (70513670)
河野 哲 朝日大学, 歯学部, 教授 (80340074)
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| Research Collaborator |
Hasegawa Tomoya
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 直接覆髄 / リン酸カルシウムセメント / 歯髄由来幹細胞 / 被蓋硬組織誘導 / 生体親和性 / バイオマテリアル / バイオアクティブ |
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| Outline of Final Research Achievements |
To develop a novel α-TCP / Te-CP cement, 2 M sodium dihydrogen phosphate solution was selected for the cement. To examine the biocompatibility of the cement, we prepared the cement-immersed media. The calcium hydroxide (Ca(OH)2) formulation was used as a control. Effects of the eluate from these cement were examined using human dental pulp derived stem cells (hDPSC). Our results revealed that the eluate from our cement did not inhibit cell proliferation, contributing to an increase in ALP activity and in the expression of osteoblast marker genes. In addition, in the animal studies of direct pulp capping, TUNEL positive cells were prominently found in the Ca(OH)2 capped grope, however, in our cement group, there were no significant inflammation findings. In addition, hard tissue regeneration was observed in our cement group. These results suggested that our novel cements indicated both cytocompatible and tissuecompatible properties for pulpal tissue as a direct pulp capping agent.
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