| Project/Area Number |
26462903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
茂木 眞希雄 愛知学院大学, 薬学部, 准教授 (00174334)
尾関 伸明 愛知学院大学, 歯学部, 講師 (70469005)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | オートファジー / 歯髄再生 / 骨格筋幹細胞 |
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| Outline of Final Research Achievements |
In this study, we examined the upregulation of autophagy-related gene (APG) signaling during human odontoblastic differentiation derived from human skeletal muscle stem cells. In a screening experiment, the mRNA and protein levels of microtubule-associated protein 1 light chain (LC3) and APG5, together with the amounts of autophagosomes and autophagy fluxes. Treatment with siRNAs against APG5 suppressed the odontoblast differentiation.
Taken together with our previous findings, we have revealed a unique sequential cascade involving APG5, and MMP-3 in odontoblastic cell differentiation of human skeletal muscle stem cells.
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