| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
Pluripotent stem cells can differentiate into multiple cell lineages following exposure to specific microenvironmental signals. We identified unique conditions that mouse pluripotent stem cells（mpSC）to differentiate into odontogenic cell lineages. Isolated acellular mimicking extracellular matrices (mECM) were sufficient to promote cell differentiation along odontoblastic pathways. The mpSC interactions with mECMs and their subsequent differentiation was suppressed by function-blocking integrin antibody. Furthermore, we found that mpSC cultured on the mECM induced the expression and secretion of a set of specific growth factors.
These results show that mpSC odontogenic cell lineage commitment is mediated by integrin interactions with specific ECM microenvironment substrates, leading to unique autocrine growth factor secretion.
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