| Project/Area Number |
26462905
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
尾関 伸明 愛知学院大学, 歯学部, 講師 (70469005)
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| Co-Investigator(Renkei-kenkyūsha) |
Mogi Makio 愛知学院大学, 薬学部, 准教授 (00174334)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ポリリン酸 / 生体高分子 / 間葉系幹細胞 / 再生医療 / 骨芽細胞 / Osteocalcin / Osteopontin / マトリックスメタロプロテアーゼ３（MMP-3） / MMP-13 / TIMP / iPS細胞 / 象牙芽細胞 / マトリックスメタロプロテアーゼ（MMP） / MMP-3 |
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| Outline of Final Research Achievements |
In this study, small interfering RNA (siRNA) was transfected into human bone marrow or adipose tissue derived mesenchmal stem cells to investigate inorganic polyphosphate [Poly(P)] is associated with cell differentiation into osteogenic cells. These cells developed an osteogenic phenotype with increased expression of Poly(P)-induced expression of matrix metalloproteinase (MMP)-13 mRNA and protein, and increased MMP-13 activity. Poly(P) also enhanced expression of mature osteoblast markers, and increased alkaline phosphatase activity and calcification capacity in these cells. MMP-13 siRNA potently suppressed the expression of osteogenic biomarkers, and blocked osteogenic calcification.
Taken together, Poly(P)-induced MMP-13 regulates differentiation of osteogenic cells from human mesenchmal stem cells.
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