The relationship between osteocyte and bone remodeling around implant

• Project/Area Number: 26462925
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Kyushu University
• Principal Investigator: Moriyama Yasuko 九州大学, 歯学研究院, 助教 (50452769)
• Co-Investigator(Kenkyū-buntansha): 古谷野 潔 九州大学, 歯学研究院, 教授 (50195872) ; 鮎川 保則 九州大学, 大学病院, 講師 (50304697)
• Co-Investigator(Renkei-kenkyūsha): KURATA Kosaku 九州大学, 大学院工学研究院, 准教授 (00368870)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 骨細胞 / オッセオインテグレーション / インプラント / 骨リモデリング / マイクロクラック / リモデリング / 応力 / 三次元培養 / MLO-Y4

Outline of Final Research Achievements

The aim of this study was to investigate the effects of force-induced biochemical response from osteocytes in vitro and in vivo.
In vitro study, we established a loading apparatus with edged titanium plate, by which cyclic stress could be applied to the gel-embedded MLO-Y4 octeocytic cell lines. Cyclic physiological strain induced the cell death of MLO-Y4 around the plate. Real-time RT-PCR analysis showed that the expression levels of connexin43 increased under the cyclic stress. The conditioned medium obtained from cyclic stimulated culture increased ALP activity of bone marrow culture. In vivo study, we inserted titanium implant at right maxillary of 4-week-old rat. After 4 weeks of healing, we provided occlusal force to implant by prosthetic appliance and compared with another side of maxilla. Histological evaluation showed that TRAP-positive cells were observed around the implant.
These results demonstrate that osteocytes possible involvement of bone remodeling around implant.

Research Products

• [Presentation] インプラント周囲骨代謝と骨細胞の力学応答の関連 (2016)
  • Author(s): 冨田 陽子、 森山 泰子、 鮎川 保則、古谷野 潔
  • Organizer: 日本口腔インプラント学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2016-09-16
• [Presentation] Osteocyte Network Regulated by Mechanical Stress Activates Peri-implant Bone Remodeling. (2016)
  • Author(s): Y Tomita, Y Moriyama, Y Ayukawa, K Kurata, H Zhang, K Koyano.
  • Organizer: 35th Annual Meeting for the IADR
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] Stress-induced Osteocytic Expression of Connexin43 around the Dental Implant. (2015)
  • Author(s): Tomita Y, Moriyama Y, Ayukawa Y, Kurata K, Fukunaga T, Koyano K.
  • Organizer: The 63rd Annual Meeting of Association for Dental Research
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2015-10-30
  • Int'l Joint Research
• [Presentation] Osteocyte Involvement in Peri-implant Strain-related Bone Remodeling (2015)
  • Author(s): Tomita Y, Moriyama Y, Ayukawa Y, Kurata K, Fukunaga T, Takamatsu H, Koyano K.
  • Organizer: The 93rd IADR General Session & Exhibition
  • Place of Presentation: Boston, Mass USA
  • Year and Date: 2015-03-11 – 2015-03-14