Development of metal nanoparticles contained resin material that inhibit MMP activity

• Project/Area Number: 26462953
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Osaka University
• Principal Investigator: Hashimoto Masanori 大阪大学, 歯学研究科, 准教授 (00337164)
• Research Collaborator: Kawakami Hayato ; Kawai Koji
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ナノ粒子 / MMP阻害剤 / 細胞毒性 / MMP / 阻害剤 / ＭＭＰ / ナノ金属粒子 / レジン材料 / マトリックスメタロプロテアーゼ / 接着耐久性 / レジン・象牙質 / レジン修復材料 / 金ナノ粒子 / 遺伝毒性

Outline of Final Research Achievements

This study evaluated the inhibition of MMPs and toxicity of nanoparticles (NPs) as possible compounds for use in dental adhesives. The MMP assay for studying the interaction of MMPs and nanoparticles (NPs) was evaluated. The cellular responses to NPs were examined using cytotoxic assay. The mechanical properties of the experimental resin loaded with NPs were examined using thermomechanical analysis. Many NPs inhibited MMP activity at relatively low concentrations. The NPs inhibit MMPs by chelating with the Zn2+ bound in the active sites of MMPs. The mechanical properties of the resins increased when NPs were added in resins. NPs are attractive candidates for dental materials to inhibit MMPs and improve the mechanical properties of resins without cytotoxic effects to cells.

Research Products

• [Journal Article] Effect of particle size of gold nanoparticles on matrix metalloprotease inhibition, cytotoxicity and genotoxicity (2017)
  • Author(s): Hashimoto M, Kawakami H, Kawai K, Imazato S
  • Journal Title: Journal of Biomaterials and Tissue Engineering Volume: 7(2) Issue: 2 Pages: 139-146
  • DOI: 10.1166/jbt.2017.1555
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Inhibition of matrix metalloproteases and toxicity of gold and platinum nanoparticles in L929 fibroblast cells. (2016)
  • Author(s): Hashimoto M, Yamaguchi S, Sasaki J, Kawai K, Kawakami H, Iwasaki Y, Imazato S.
  • Journal Title: European Journal of Oral Sciences Volume: １２４ Issue: 1 Pages: 68-74
  • DOI: 10.1111/eos.12235
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Matrix metalloproteases inhibition and biocompatibility of gold and platinum nanoparticles. (2016)
  • Author(s): Hashimoto M, Kawai K, Kawakami H, Imazato S.
  • Journal Title: Journal of Biomedical Materials Research: Part A Volume: １０４ Issue: 1 Pages: 209-217
  • DOI: 10.1002/jbm.a.35557
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Investigation of the cytotoxicity of aluminum oxide nanoparticles and nanowires and their localization in L929 fibroblasts and RAW264 macrophages. (2016)
  • Author(s): Hashimoto M, Sasaki J, Imazato S.
  • Journal Title: Journal of Biomedical Materials Research: Part B Volume: １０４ Issue: 2 Pages: 241-252
  • DOI: 10.1002/jbm.b.33377
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Investigation of aluminum oxide nanoparticles and nanowires and their localization in L929 fibroblasts and RAW264 macrophages. (2015)
  • Author(s): Hashimoto M, Sasaki J, Imazato S
  • Journal Title: Journal of Biomedical Materials Research: Part B Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytotoxic and genotoxic characterization of aluminum and silicon oxide nanoparticles in macrophages. (2015)
  • Author(s): Hashimoto M, Sasaki J.I, Yamaguchi S, Kawai K, Kawakami H, Iwasaki Y, Imazato S
  • Journal Title: Dental Materials Volume: 31 Issue: 5 Pages: 556-564
  • DOI: 10.1016/j.dental.2015.02.009
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 金属ナノ粒子を添加した高弾性率, 抗菌性および抗酸化性を有する樹脂材料の開発. (2016)
  • Author(s): 川上隼人, 河合功治, 橋本正則. 今里 聡.
  • Organizer: 第67回日本歯科理工学会学術講演会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県福岡市）
  • Year and Date: 2016-04-15
• [Presentation] 金ナノ粒子の粒径がMMP阻害活性および細胞・遺伝毒性に与える影響. (2016)
  • Author(s): 橋本正則, 川上隼人, 河合功治, 今里 聡.
  • Organizer: 第67回日本歯科理工学会学術講演会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県福岡市）
  • Year and Date: 2016-04-15
• [Presentation] 金および白金ナノ粒子のＭＭＰ活性抑制効果および細胞・遺伝毒性 (2015)
  • Author(s): 橋本正則、川上隼人、河合功治、岩崎泰彦、今里 聡
  • Organizer: 第３７回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ（京都府・京都市）
  • Year and Date: 2015-11-09
• [Presentation] 貴金属ナノ粒子を添加した高弾性率な樹脂材料の開発 (2015)
  • Author(s): 川上隼人、河合功治、橋本正則、今里 聡
  • Organizer: 第３７回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ（京都府・京都市）
  • Year and Date: 2015-11-09
• [Presentation] 金および白金ナノ粒子のMMP-1抑制効果と線維芽細胞に対する毒性作用 (2015)
  • Author(s): 44.橋本正則、佐々木淳一、山口 哲、今里 聡
  • Organizer: 日本歯科保存学会２０１５年春期学術大会（第１４２回）
  • Place of Presentation: 北九州国際会議場（福岡県・北九州市）
  • Year and Date: 2015-06-25
• [Presentation] 金および白金ナノ粒子のMMP-8,9に対する抑制作用と細胞毒性 (2015)
  • Author(s): 橋本正則，佐々木淳一，山口 哲，河合功治，川上隼人，岩崎泰彦，今里 聡
  • Organizer: 第６５回日本歯科理工学会学術講演会
  • Place of Presentation: 仙台市 仙台市情報・産業プラザ
  • Year and Date: 2015-04-11 – 2015-04-12
• [Presentation] 金および白金ナノ粒子のＭＭＰ－８，９に対する抑制作用と細胞毒性 (2015)
  • Author(s): 橋本正則、佐々木淳一、今里 聡
  • Organizer: 第６５回日本歯科理工学会学術講演会
  • Place of Presentation: 仙台市情報・産業プラザ（宮城県・仙台市）
  • Year and Date: 2015-04-11
• [Presentation] アルミナナノ材料の形状が線維芽細胞およびマクロファージに与える影響 (2014)
  • Author(s): 橋本正則、佐々木淳一、今里 聡
  • Organizer: 第６４回日本歯科理工学会学術講演会
  • Place of Presentation: 広島市アステールプラザ
  • Year and Date: 2014-10-05 – 2014-10-06
• [Patent(Industrial Property Rights)] 金微粒子マトリックスメタロプロテアーゼ阻害剤 (2017)
  • Inventor(s): 橋本正則
  • Industrial Property Rights Holder: 橋本正則
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-011377
  • Filing Date: 2017-01-23
• [Patent(Industrial Property Rights)] 金微粒子マトリックスプロテアーゼ阻害剤 (2015)
  • Inventor(s): 橋本正則
  • Industrial Property Rights Holder: 橋本正則
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-01-23