Mechanism of response of periodontal tissue to vibrational stimulation: molecular basis and clinical application
| Project/Area Number |
26462963
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
Chiba Mirei 東北大学, 歯学研究科, 講師 (10236820)
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| Co-Investigator(Kenkyū-buntansha) |
五十嵐 薫 東北大学, 歯学研究科, 教授 (70202851)
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| Project Period (FY) |
2014-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 振動 / 周波数 / 加速度 / 骨芽細胞 / iPS細胞 / 歯科矯正学的歯の移動 / 歯周組織 / 骨密度 / 破骨細胞 / 振動刺激 / 骨形成 / 血流 / ERK1/2 / アクチンストレスファイバー / ビンキュリン / 接着斑 / リモデリング / メカニカルストレス / 配向性 |
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| Outline of Final Research Achievements |
It is known that periodontal tissue is activated by vibrational stimulation, but the mechanism is unknown.
In this study, mouse osteoblast-like MC3T3-E1 cells were cultured and subjected to vibrational stimulation. Vibrational stimulation induced osteoblast differentiation and the upregulation of osteogenic gene expression, and the development of actin stress fibers in the cells was enhanced. These effects varied with vibration parameters: frequency and particularly, acceleration.
The effects of vibrational stimulation in vivo were also analyzed by applying vibrational stimulation to teeth and periodontal tissue in model rats. Time-dependent changes in bone and tissue in the anesthetized animals were analyzed. Micro-computed tomography and ultrasound revealed quantitative effects.
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| Academic Significance and Societal Importance of the Research Achievements |
歯科矯正治療には長期間を要する事が多く、治療の長期化に伴って齲蝕（むし歯）や歯周病などの問題が発生し、患者の精神的負担も大きい。また、治療期間の長期化は、重篤な副作用のひとつである歯根吸収の発現と関連性が高いことが報告され、治療期間を短縮する方法が求められている。
本研究では、振動刺激により歯周組織を活性化する刺激条件を解明した。副作用がなく生理的かつ速やかな骨改造制御の開発は、治療における骨吸収と骨形成の制御（矯正治療速度制御、副作用防止、動的治療期間および保定期間の短縮、術後経過の安定）、歯科インプラント植立時の新生骨再生、抜歯後の治癒促進などへの応用が期待される。
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Report
(6 results)
Research Products
(41 results)
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[Presentation] The Novel Bisphosphonate Regulates Osteoclastogenesis, Osteogenesis, and Adipogenesis2017
Author(s)
Mirei Chiba, Aiko Takizawa, Takeru Ota, Mayumi Yasuda, Keiko Suzuki, Kaoru Igarashi, Hisashi Shinoda
Organizer
95th General Session & Exhibition of the IADR/46th AADR/41st CADR
Place of Presentation
Moscone West, San Francisco, CA 94103, USA.
Year and Date
2017-03-22
Related Report
Int'l Joint Research
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[Presentation] Effects of Cyclic Tensile Strain on Mouse Stem Cells2017
Author(s)
Mayumi YASUDA, Mirei CHIBA, Shiho KATSUKI, Michiko MATSUOKA, Kaoru IGARASHI, Haruhide HAYASHI
Organizer
95th General Session & Exhibition of the IADR/46th AADR/41st CADR
Place of Presentation
Moscone West, San Francisco, CA 94103, USA.
Year and Date
2017-03-22
Related Report
Int'l Joint Research
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[Presentation] A novel bisphosphonate, [4- (methylthio) phenylthio] methanebisphosphonate, facilitates bone formation by local administration to the alveolar bone in rats2017
Author(s)
Aiko Takizawa, Mirei Chiba, Yuka Narusawa, Makoto Nishimura, Takeru Ota, Mayumi Yasuda, Keiko Suzuki, Haruhide Hayashi, Kaoru Igarashi, Hisashi Shinoda
Organizer
The 2017 Japan-NIH joint Symposium
Place of Presentation
Seiryo Auditorium, Seiryo Campus , Tohoku University, Japan.
Year and Date
2017-02-15
Related Report
Int'l Joint Research
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[Presentation] The effects of mechanical stretch on mouse iPS cell line2015
Author(s)
Mayumi YASUDA, Mirei CHIBA, Takeru OTA, Aiko TAKIZAWA, Kaoru IGARASHI
Organizer
10th International Symposium on Advanced Science and Technology in Experimental Mechanics (10th ISEM '15-Matsue)
Place of Presentation
Kunibikimesse, Shimane Prefectural Convention Center, Matsue, Japan
Year and Date
2015-11-01
Related Report
Int'l Joint Research
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