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Development of novel bone graft materials by combining scaffold-free cell construct and freeze dry technique

Research Project

Project/Area Number 26462969
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionOsaka University

Principal Investigator

Sasaki Jun-Ichi  大阪大学, 歯学研究科, 助教 (50530490)

Co-Investigator(Kenkyū-buntansha) 今里 聡  大阪大学, 歯学研究科, 教授 (80243244)
Research Collaborator YOSHIMOTO Itsumi  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords生体材料学 / 再生歯学 / 骨補填材 / 細胞集合体 / 歯学 / 組織工学 / 骨
Outline of Final Research Achievements

The objective of this study was to develop the bone graft materials by combining scaffold-free cell construct fabrication system and freeze dry technique. With the usage of appropriate pretreatments, bone-like constructs, fabricated through osteoinduction of aggregate of bone marrow stromal cells, were able to maintain extracellular matrix and mineralized matrices even after being freeze-dried. The freeze-dried bone like constructs achieved in this study are expected to be used as a useful biomaterial for bone regenerative medicine.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (23 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Invited: 3 results) Remarks (6 results)

  • [Int'l Joint Research] ミシガン大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ミシガン大学歯学部(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Scaffold-free fabrication of osteoinductive cellular constructs using mouse gingiva-derived induced pluripotent stem cells.2016

    • Author(s)
      Okawa H, Kayashima H, Sasaki JI, Miura J, Kamano Y, Imazato S, Yatani H, Matsumoto T, Egusa H.
    • Journal Title

      Stem Cells International

      Volume: 2016 Issue: 1 Pages: 1-11

    • DOI

      10.1155/2016/6240794

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fabrication of biomimetic bone tissue using mesenchymal stem cell-derived three-dimensional constructs incorporating endothelial cells.2015

    • Author(s)
      Sasaki JI, Hashimoto M, Yamaguchi S, Itoh Y, Yoshimoto I, Matsumoto T, Imazato S.
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 6 Pages: e0129266-e0129266

    • DOI

      10.1371/journal.pone.0129266

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oriented bone formation using biomimetic fibrin hydrogels with three-dimensional patterned bone matrices.2015

    • Author(s)
      Sasaki JI, Matsumoto T, Imazato S.
    • Journal Title

      Journal of Biomedical Materials Research Part A

      Volume: 103 Issue: 2 Pages: 622-627

    • DOI

      10.1002/jbm.a.35212

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 三次元細胞集合体を応用した骨様組織のin vitro創製.2015

    • Author(s)
      佐々木淳一,今里 聡.
    • Journal Title

      バイオマテリアル-生体材料-

      Volume: 33 Pages: 41-44

    • Related Report
      2014 Research-status Report
  • [Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro.2014

    • Author(s)
      Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
    • Journal Title

      Stem cells and development.

      Volume: 23 Issue: 18 Pages: 2156-2169

    • DOI

      10.1089/scd.2013.0344

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 試作二層性GTR用メンブレンの組織再生誘導能の評価.2017

    • Author(s)
      吉本いつみ、壷井莉理子、北川晴朗、佐々木淳一、今里 聡.
    • Organizer
      第69回日本歯科理工学会学術講演会
    • Place of Presentation
      日本歯科大学生命歯学部富士見ホール(東京都千代田区)
    • Year and Date
      2017-04-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 層構造を有する歯周組織再生用PLGAメンブレンの開発.2016

    • Author(s)
      吉本いつみ、佐々木淳一、壷井莉理子、伊藤善博、今里 聡.
    • Organizer
      第67回日本歯科理工学会学術講演会
    • Place of Presentation
      九州大学医学部百年講堂(福岡県福岡市)
    • Year and Date
      2016-04-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 層構造を有する歯周組織再生用 PLGAメンブレンの開発.2016

    • Author(s)
      吉本いつみ、佐々木淳一、壷井莉理子、伊藤善博、今里 聡.
    • Organizer
      第67回日本歯科理工学会学術講演会
    • Place of Presentation
      九州大学医学部百年講堂(福岡県福岡市)
    • Year and Date
      2016-04-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 失われた歯髄は再生できるか.2015

    • Author(s)
      佐々木淳一.
    • Organizer
      平成27年度大阪府歯科医師会学術講演会
    • Place of Presentation
      大阪府歯科医師会館(大阪府大阪市)
    • Year and Date
      2015-07-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 歯髄幹細胞を用いた棒状三次元細胞集合体の作製.2015

    • Author(s)
      伊藤善博、佐々木淳一、林 美加子、松本卓也、今里 聡.
    • Organizer
      第65回日本歯科理工学会学術講演会
    • Place of Presentation
      仙台市情報・産業プラザ(宮城県仙台市)
    • Year and Date
      2015-04-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Fabrication of biomimetic bone-like tissue by using 3D cell constructs.2014

    • Author(s)
      Sasaki JI, Matsumoto T, Imazato S.
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      東京都
    • Year and Date
      2014-11-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Fabrication of biomimetic bone-like tissue by using 3D cell constructs.2014

    • Author(s)
      Sasaki JI, Matsumoto T, Imazato S.
    • Organizer
      The 22nd Annual Meeting of the Korean Society for Biomaterials
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2014-11-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 血管内皮細胞の導入による三次元細胞集合体内の生細胞維持の試み.2014

    • Author(s)
      松田真悠、濱本大貴、吉崎文彦、佐々木淳一、今里 聡.
    • Organizer
      大阪大学歯学会第118回例会
    • Place of Presentation
      吹田市
    • Year and Date
      2014-07-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Fabrication of scaffold-free 3D cell constructs for bone regeneration.2014

    • Author(s)
      Sasaki JI, Imazato S.
    • Organizer
      Kyungpook-Osaka University International Symposium, 2014 "New Horizon of Basic and Clinical Research in Dentistry"
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2014-05-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 細胞集合体の凍結乾燥処理による幹細胞由来新規骨補填材の作製.2014

    • Author(s)
      吉本いつみ,佐々木淳一,松本卓也,今里 聡.
    • Organizer
      第63回日本歯科理工学会学術講演会
    • Place of Presentation
      東京都
    • Year and Date
      2014-04-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪大学大学院歯学研究科顎口腔機能再建学講座(歯科理工学教室)

    • URL

      http://web.dent.osaka-u.ac.jp/~techno/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学研究者総覧

    • URL

      http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=2796&a2=0000007&o=affiliation&sm=affiliation&sl=ja&sp=1

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学大学院歯学研究科顎口腔機能再建学口座(歯科理工学教室)

    • URL

      http://web.dent.osaka-u.ac.jp/~techno/Welcome.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪大学 研究者総覧

    • URL

      http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=2796&a2=0000007&k=%E4%BD%90%E3%80%85%E6%9C%A8&kc=1&o=affiliation&pp=50&sm=affiliation&sl=ja&sp=1

    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪大学大学院歯学研究科顎口腔機能再建学講座(歯科理工学教室)

    • URL

      http://web.dent.osaka-u.ac.jp/~techno/Welcome.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪大学 研究者総覧

    • URL

      http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=2796&a2=0000007&o=affiliation&sm=affiliation&sl=ja&sp=1

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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