Basic research toward the development of periodontal tissue regeneration implant using dentin sialophosphoprotein.
Project/Area Number |
26462982
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dental engineering/Regenerative dentistry
|
Research Institution | Tsurumi University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
山本 竜司 鶴見大学, 歯学部, 助教 (20410053)
唐木田 丈夫 鶴見大学, 歯学部, 学内講師 (40367305)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 歯 / タンパク質 / 生理活性物質 / 再生 / 遺伝子 / 歯髄 / 象牙芽細胞 / 象牙質 |
Outline of Final Research Achievements |
We purified TGF-β-unbound or -bound DPP and DSP. The TGF-β isoform bound to DPP and DSP was identified as being TGF-β1 by both ELISA and LC-MS/MS analysis. DPP and DSP rescued the loss of TGF-β1 activity suggesting that they help retain TGF-β1 activity in porcine dentin. We further fractionated DSPP-derived proteins from the dental pulp of developing porcine incisors. DSP was identified, but little DPP could be detected. Expression of the full-length Dspp mRNA by quantitative real-time polymerase chain reaction analysis was significantly higher in odontoblasts than in pulp, while expression of the DSP-only mRNA was almost equal in odontoblasts and in the pulp. Expression of the full-length Dspp mRNA was also significantly higher than the expression of DSP-only mRNA in odontoblasts. We conclude that the alternative polyadenylation of specific mRNAs of DSPP is regulated differently in pulp and differentiated odontoblasts.
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Report
(4 results)
Research Products
(14 results)