The study of the mandibular condyle regeneration using the cell sheet

• Project/Area Number: 26462987
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: NAKATSUKA Michiko 大阪歯科大学, 歯学部, 講師 (70368158)
• Co-Investigator(Kenkyū-buntansha): 乾 千珠子 (山本千珠子 / 乾 千珠子(山本千珠子)) 大阪大学, 歯学研究科, 助教 (00419459) ; 隈部 俊二 大阪歯科大学, 歯学部, 准教授 (30288774) ; 細矢 明宏 松本歯科大学, 歯学部, 講師 (70350824)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨髄由来細胞 / 軟骨分化 / 下顎頭 / 軟骨 / 再生

Outline of Final Research Achievements

To elucidate the chondrocyte differentiation ability of bone marrow-derived mesenchymal stem cells, we experimented with the cells that formed spheroids in vitro. The cells were cultured into cryo vials (100000 cells/ml) and incubated in culture media (1% FBS, 1% insulin - transferrin - selenite (ITS), dexamethasone (0.1μM), sodium pyruvate (1mM), TGFβ-3 (10 ng/ml-containing). The differentiation of the spheroids that the cells formed (day 21 specimen) was assayed. Cell differentiation was evaluated by Alcian blue staining. We also examined immunohistochemically by using Sox9 and aggrecan antibodies. In the experimental group,these structures showed positive reaction of Alcian blue, Sox9, and aggrecan. On the other hand, there were no evidences of chondrocyte differentiation in the control group. Therefore, these results suggest that spheroid formation of bone marrow-derived mesenchymal stem cells might be useful in chondrocyte regeneration therapy.

Research Products

• [Presentation] 間葉系幹細胞の三次元培養による軟骨再生に向けた基礎研究. (2017)
  • Author(s): 中塚 美智子、細矢 明宏、隈部 俊二、田村 功．
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学（長崎県・長崎市）
• [Presentation] Bone marrow-derived mesenchymal stem cells are differentiated into chondrocyte-like cells by forming spheroids in vitro. (2016)
  • Author(s): NAKATSUKA M, HOSOYA A, KUMABE S, TAMURA I.
  • Organizer: 第58回歯科基礎医学会学術大会・総会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
• [Presentation] 骨髄由来間葉系幹細胞の軟骨細胞への分化誘導 (2015)
  • Author(s): 中塚美智子、松田哲史、細矢明宏、隈部俊二
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟コンベンションセンター）
  • Year and Date: 2015-09-11