| Project/Area Number |
26462988
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
関野 徹 大阪大学, 産業科学研究所, 教授 (20226658)
岡田 正弘 岡山大学, 医歯薬学総合研究科, 准教授 (70416220)
本田 義知 大阪歯科大学, 歯学部, 准教授 (90547259)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 酸化チタン / チタニアナノチューブ / PDT / DDS / 活性酸素 / バイオマテリアル / ナノチューブ |
|---|
| Outline of Final Research Achievements |
Since one of the potential applications of titanium oxide nano-tubes (TNT) is to photodynamic therapy (PDT), the effects of reactive oxygen species (ROS) formed by TNT irradiated with ultraviolet (UV) rays were evaluated in HeLa cells.
An oxidative stress fluorescence probe was then used to visualize oxidative stress in HeLa cells, and revealed that the ROS formed by TNT led to oxidative stress in HeLa cells. A flow cytometric analysis was performed to characterize the cell status and distinguish between viable and unviable cells. Polyethylene glycol (PEG) modifications to the surfaces of TNT effectively facilitated adherence to cells, and the UV irradiation of HeLa cells during their incubation in culture medium containing PEG-modified TNT induced oxidative stress, thereby suppressing DNA repair.
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